MCM5 antibody

Principal name

MCM5 antibody

Alternative names for MCM5 antibody

CDC46 homolog, P1-CDC46, CDC46, DNA replication licensing factor MCM5, minichromosome maintenance complex component 5

SwissProt ID

P29496 (Yeast), P33992 (Human), P49718 (Mouse), Q0V8B7 (Bovin)

Gene ID

4174 (MCM5)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Xen (Xenopus), Chk (Chicken), Mky (Monkey), Rb (Rabbit), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of MCM5 antibody

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-induced Stat1 Alpha transcription activation.

General readings

Ohtani,K., et al., (1999) Oncogene 18 (14), 2299-2309

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM5 antibody

Jurkat cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM5 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

MCM5 antibody

Western blot analysis of extracts from HepG2 cells, using MCM5 antibody (#TA312325).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM5 antibody

Western blot analysis of extracts from A549 cells, using MMP-11 antibody (#TA312326).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM5 antibody

Western blot analysis of extracts of various cell lines, using MCM5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM5 antibody

WB Suggested Anti-MCM5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM5 antibody

Western blot analysis on HepG2 cell lysate using MCM5 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM5 antibody

Immunohistochemistry (IHC) analyzes of MCM5 antibody (Cat.-No.: AP06217PU-N) in paraffin-embedded human tonsil tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MCM5 antibody

Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti huamn MCM5 (SM1483P/PT) Mouse IgG2b CRCT5.1 Purified Hu C, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

MCM5 antibody

Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti huamn MCM5 (SM1483P/PT) Mouse IgG2b CRCT5.1 Purified Hu C, P, WB
20 µg / €240.00
  Acris Antibodies GmbH

MCM5 antibody

MCM5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MCM5 antibody

MCM5 Mouse IgM 3A7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

MCM5 antibody

MCM5 monoclonal antibody (M03), clone 2H8. Western Blot analysis of MCM5 expression in Hela NE(Cat # L013V3 ). Mouse IgG2a 2H8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

MCM5 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using MCM5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

MCM5 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using MCM5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

MCM5 antibody

Human HepG2; WB Suggested Anti-MCM5 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; MCM5 antibody - N-terminal region (ARP36654_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

MCM5 antibody

MCM5 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

MCM5 antibody

Immunohistochemical analysis of CDC46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MCM5 antibody

Immunohistochemical analysis of CDC46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MCM5 antibody

Immunohistochemical analysis of CDC46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MCM5 antibody

Immunohistochemistry-Paraffin: MCM5 Antibody (CRCT5.1) [NB100-65643] - Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Humann MCM5. Mouse IgG2b CRCT5.1 Aff - Purified Hu P
20 µg / €340.00
  Novus Biologicals Inc.

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