MCM7 antibody

Principal name

MCM7 antibody

Alternative names for MCM7 antibody

CDC47, MCM2, CDC47 homolog, P1.1-MCM3, minichromosome maintenance complex component 7, DNA replication licensing factor MCM7

SwissProt ID

P33993 (Human), Q3ZBH9 (Bovin), Q61881 (Mouse)

Gene ID

4176 (MCM7)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Xen (Xenopus), Bov (Bovine), African clawed frog, Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of MCM7 antibody

MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

General readings

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of
replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007.
2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a
product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68:
226-230, 1995.
3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D.,
Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G.,
Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle
arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM7 antibody

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308526 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

Western blot analysis of extracts of variouscell lines, using MCM7 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml] Mouse IgG2a 6C2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MCM7 antibody

Predicted band size: 81 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 50 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM7 (C-term) antibody

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody against MCM7.  Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

WB Suggested Anti-MCM7 Antibody Titration: 1.25 ug/ml; Positive Control: Daudi cell lysateMCM7 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells Rabbit IgG Purified Bov, Eq, GP, Hu, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

WB Suggested Anti-MCM7 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: Daudi cell lysate MCM7 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

WB Suggested Anti-MCM7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 293T cell lysate MCM7 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

Host: Rabbit; Target Name: MCM7; Sample Tissue: Thyroid Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCM7 antibody

Figure 1. Western blot analysis of MCM7 expression in HeLa cells (Lane 1), A-431 cells (Lane 2), Jurkat cells (Lane 3) and C2C12 cells (Lane 4) using MCM7 antibody (AM00302AF-N). Mouse IgG1 DCS-141 Azide Free Hu, Ms P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MCM7 antibody

MCM7 Mouse IgG1 M3 Purified Hu ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MCM7 (full length) antibody

Immunocytochemical detection of MCM7 on 4% PFA fixed HeLa cells with AM26600AF-N. Mouse IgG2a 4B4 Azide Free Hu, Ms ICC/IF, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MCM7 (C-term) antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µl / €470.00
  Acris Antibodies GmbH

MCM7 (C-term) antibody

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody against MCM7.  Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms WB
50 µl / €190.00
  Acris Antibodies GmbH

MCM7 (N-term) antibody

Western blot analysis of MCM7 Antibody (N-term) Cat.-No AP52641PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the MCM7 antibody detected the MCM7 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MCM7 antibody

Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody. DM340 Mouse IgG1 47DC141 Purified Hu C, IP, P, WB
1 ml / €700.00
  Acris Antibodies GmbH

MCM7 antibody

Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody. DM340 Mouse IgG1 47DC141 Purified Hu C, IP, P, WB
0.5 ml / €450.00
  Acris Antibodies GmbH

MCM7 antibody

Immunofluorescence of purified MaxPab antibody to MCM7 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

MCM7 antibody

MCM7 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MCM7 antibody

MCM7 MaxPab rabbit polyclonal antibody. Western Blot analysis of MCM7 expression in mouse lung. Rabbit Serum Hu, Ms E(detection), WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

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