MCP1 / CCL2 antibody

Principal name

MCP1 / CCL2 antibody

Alternative names for MCP1 / CCL2 antibody

C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Monocyte chemotactic protein 1

SwissProt ID

P10148 (Mouse), P13500 (Human), P14844 (Rat), P28291 (Bovin), P42831 (Pig)

Gene ID

6347 (CCL2), 24770 (Ccl2), 20296, 281043

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Rabbit, Hamster, Sheep, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Functional assay (FN), ELISA (detection) (E(detection)), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Luminex (LMNX)

Background of MCP1 / CCL2 antibody

Monocyte chemotactic protein-1 (MCP-1) belongs to the chemokine family. Chemokines are small secreted molecules containing 4 conserved cysteine residues and 2 disulfide linkages. The first two cysteine residues of chemokine molecules may be in one of the following configurations, CC or CXC and this defines the two major chemokine sub-families. MCP-1 is a CC chemokine and its recent designation is chemokine ligand 2, (CCL2). MCP-1 is expressed by monocytes, dendritic cells, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells, osteoblastic cells, and human pulmonary type-2-like epithelial cells in response to the cytokines IL-1, TNF-a, IFN-g, as well as bacterial or viral products and mitogens. MCP-1 exhibits chemotactic activity for monocytes/macrophages, basophils, T lymphocytes, particularly memory T cells and natural killer cells and neural stem cells. Elevated levels of MCP-1 are detected during inflammation and immune responses. MCP-1 activity is also important in wound healing as shown by delayed wound repair in MCP-1deficient mice. MCP-1 is implicated in the pathogenesis of disease states that involve monocyte/macrophage infiltrates, such as psoriasis, rheumatoid arthritis and recruitment of monocytes to the arterial wall in atherosclerosis. In addition to its chemotactic function, MCP-1 also induces the expression of IL-10 from macrophages, which favours a TH2 immune response. MCP-1 is a glycopeptide containing one potential N-linked glycosylation site. The extent of MCP-1 glycosylation influences its chemotactic potency and half-life in vivo. The glycosylated form of MCP-1 exhibits lower specific chemotactic activity for both lymphocytes and monocytes than the non-glycosylated form, however the non-glycosylated form rapidly loses activity at 37degrees Celsius. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

General / background references:Miller MD & Krangel MS Biology and biochemistry of the chemokines: a family of chemotactic and inflammatory cytokines. Crit Rev Immunol 12:17-46 (1992). PubMed PMID: 1418604
Mukaida N et al. Properties of pro-inflammatory cell type-specific leukocyte chemotactic cytokines, interleukin 8 (IL-8) and monocyte chemotactic and activating factor (MCAF). Microbiol Immunol 36:773-89 (1992). PubMed PMID: 1474930
Matsushima K et al. Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J Exp Med 169:1485-90 (1989). PubMed PMID: 2926331
Furutani Y et al. Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF). Biochem Biophys Res Commun 159:249-55 (1989). PubMed PMID: 2923622

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCP1 / CCL2 (C-term) antibody

Western blot analysis of CCL2 Antibody (C-term) (Cat. #TA324861) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab. Rabbit Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Western Blot: MCP1 Antibody (2D8) [TA336914] - Western blot analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa) Mouse IgG1 2D8 Purified Hu, Ms, Prim C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CCL2 Rabbit Polyclonal antibody. (TA590148) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 antibody Cat.-No AM06749PU-N with DAB staining. Mouse IgG1 2D8 Purified Hu, Mky, Ms E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MCP1 / CCL2 antibody

Western blot analysis of MCP-1 in LPS treated RAW cell lysate. Rabbit Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Anti-human MCP-1 by WB shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was incubated with Rockland's, Inc. Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (p/n 611-143-122) diluted 1:20,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Western blot analysis of CCL2 in rat spleen tissue lysate with CCL2 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Staining pf mouse thioglycolate-induced peritoneal exudate cells (PECs) stimulated with LPS in the presence of Brefeldin A for 24 hours with Anti-Mouse F4/80 Antigen FITC and intracellularly stained with Rat IgG2a K Isotype Control (left) or with Anti-CCL2 (MCP-1) PE (right). Hamster 2H5 Purified Hu, Ms, Rt E, F, P, WB
50 µg / €120.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

MCP1 / CCL2 Hamster IgG 4E2 Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

MCP1 / CCL2 Mouse 5D3-F7 Purified Hu E, F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/25-50  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CCL2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CCL2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x100) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

To detect hMCP-1/MCAF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-1/MCAF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

To detect hMCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-1(MCAF) (TA328374) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

To detect mJE(MCP-1) by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mJE(MCP-1). Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

To detect mJE(MCP-1) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine JE(MCP-1)(TA328524) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1). Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

To detect Rat MCP-1(MCAF) by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (TA328588) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF). Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Western Blot: MCP1 Antibody [TA336723] - WB analysis of MCP1 in Hela whole cell lysates. Rabbit Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Western Blot: MCP1 Antibody [TA336724] - Western Blot on TNF alpha treated PC12 lysates. Rabbit Purified Hu, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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