MCP1 / CCL2 antibody

Principal name

MCP1 / CCL2 antibody

Alternative names for MCP1 / CCL2 antibody

C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Monocyte chemotactic protein 1

SwissProt ID

P10148 (Mouse), P13500 (Human), P14844 (Rat), P28291 (Bovin), P42831 (Pig)

Gene ID

6347 (CCL2), 24770 (Ccl2), 20296, 281043

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Rabbit, Sheep, Rat, Hamster

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), ELISA (detection) (E(detection)), Neutralisation (NEUT), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Luminex (LMNX)

Background of MCP1 / CCL2 antibody

The murine JE gene encodes the monocyte-specific cytokine monocyte chemotactic protein 1 (MCP-1). MCP-1 is a CC chemokine of 76 amino acids (~11 kDa) and is chemotactic for monocytes and basophils but not neutrophils and eosinophils. MCP-1 is expressed by smooth muscle cells (SMC), macrophages, endothelial cells, keratinocytes and fibroblasts in response to inflammatory stimuli such as interleukin 1β and tumor necrosis factor α. MCP-1 has been implicated in a variety of inflammatory processes, including inflammatory bowel disease, rheumatoid arthritis, asthma, nephritis, and parasitic and viral infections. MCP-1 antigen is not detected in the endothelium or SMC of normal arteries. MCP-1 has also been shown to exhibit biological activities other than chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer)
MCP-1 signals via the CCR2 receptor, and is critical for aneurysm formation because of its stability to recruit leukocytes. These leukocytes produce extracellular matrix-degrading MMPs, thereby inductin aortic remodelling and dilatation. Interleukin-6 is also involved in this amplification loop accelerating vascular inflammation. MCP-/- mice display significantly delayed wound re-epithelialization, and also delayed wound angiogenesis.

General readings

Boring, L.et al.( 1996). J Biol Chem.271:7551.

Capsoni, F. et al.( 1989) J Immunol Methods.120:125.

Hsu, S.M. et al. (1981). Am J Clin Pathol.75:734.

Hsu, S.M. et al. (1981)J Histochem Cytochem. 29:577.

Matsushima, K. et al. (1989)Cytokine.1:2.

Peri, G. et al. (1994)J Immunol Methods. 174:249.

Prussin, C. and Metcalfe, D.D. (1995) J Immunol Methods.188:117.

Rollins, B.J. et al.( 1989) Mol Cell Biol. 9:4687.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCP1 / CCL2 (C-term) antibody

  Rabbit polyclonal CCL2 Antibody (C-term)  
Western blot analysis of CCL2 Antibody (C-term) (Cat. #TA324861) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab. Rabbit Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Mouse Monoclonal CCL2/MCP1 Antibody (2D8)  
Western Blot: MCP1 Antibody (2D8) [TA336914] - Western blot analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa) Mouse IgG1 2D8 Purified Hu, Ms, Prim C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Rabbit Polyclonal MCP1 Antibody  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CCL2 Rabbit Polyclonal antibody. (TA590148) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 antibody Cat.-No AM06749PU-N with DAB staining. Mouse IgG1 2D8 Purified Hu, Mky, Ms E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MCP1 / CCL2 antibody

  Rabbit polyclonal anti-CCL2 (MCP-1) antibody  
Western blot analysis of MCP-1 in LPS treated RAW cell lysate. Rabbit Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Rabbit polyclonal anti-MCP-1 antibody  
Anti-human MCP-1 by WB shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was incubated with Rockland's, Inc. Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (p/n 611-143-122) diluted 1:20,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Rabbit Polyclonal CCL2 Antibody  
Western blot analysis of CCL2 in rat spleen tissue lysate with CCL2 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Hamster Anti-CCL2 (MCP-1) Purified (50 ug)  
Staining pf mouse thioglycolate-induced peritoneal exudate cells (PECs) stimulated with LPS in the presence of Brefeldin A for 24 hours with Anti-Mouse F4/80 Antigen FITC and intracellularly stained with Rat IgG2a K Isotype Control (left) or with Anti-CCL2 (MCP-1) PE (right). Hamster 2H5 Purified Hu, Ms, Rt E, F, P, WB
50 µg / €120.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Hamster Anti-Mouse CCL2 (MCP-1) Purified (50 ug)  
MCP1 / CCL2 Hamster IgG 4E2 Purified Ms E
50 µg / €120.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Mouse Anti-Human CCL2 (MCP-1) Purified (50 ug)  
MCP1 / CCL2 Mouse 5D3-F7 Purified Hu E, F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Anti-CCL2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/25-50  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CCL2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Anti-CCL2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CCL2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x100) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Biotinylated Anti-Human MCP-1 Rabbit Polyclonal Antibody  
To detect hMCP-1/MCAF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-1/MCAF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Anti-Human MCP-1 Rabbit Polyclonal Antibody  
To detect hMCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-1(MCAF) (TA328374) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Biotinylated Anti-Murine JE/MCP-1 Rabbit Polyclonal Antibody  
To detect mJE(MCP-1) by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mJE(MCP-1). Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Anti-Murine JE/MCP-1 Rabbit Polyclonal Antibody  
To detect mJE(MCP-1) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine JE(MCP-1)(TA328524) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1). Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Biotinylated Anti-Rat MCP-1 Rabbit Polyclonal Antibody  
To detect Rat MCP-1(MCAF) by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Anti-Rat MCP-1 Rabbit Polyclonal Antibody  
To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (TA328588) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF). Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Rabbit Polyclonal CCL2/MCP1 Antibody  
Western Blot: MCP1 Antibody [TA336723] - WB analysis of MCP1 in Hela whole cell lysates. Rabbit Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCP1 / CCL2 antibody

  Rabbit Polyclonal CCL2/MCP1 Antibody  
Western Blot: MCP1 Antibody [TA336724] - Western Blot on TNF alpha treated PC12 lysates. Rabbit Purified Hu, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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