MDH2 antibody
Principal name
MDH2 antibody
Alternative names for MDH2 antibody
Malate dehydrogenase, mitochondrial
Ncbi ID
9823, 9606, NP_005909, NP_005909.2, NP_032643.2, NM_008617, NM_031151, NM_005918
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MDH2 antibody
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Thummel CS., et al. (2010) Dev Dyn. 239(3):954-64.
Lee JS., et al (2002) J Basic Microbiol. 42(4):238-45.
Detection limit for recombinant GST tagged MDH2 is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MDH2 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MDH2 is approximately 0.3ng/ml as a capture antibody.
Rat kidney; MDH2 antibody - C-terminal region (ARP48286_T100) validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.; MDH2 antibody - C-terminal region (ARP48286_T100) in Rat kidney cells using Western Blot
Human HepG2; WB Suggested Anti-MDH2 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; MDH2 antibody - C-terminal region (ARP48286_T100) in Human HepG2 cells using Western Blot
MDH2 monoclonal antibody (M01), clone 1G12. Western Blot analysis of MDH2 expression in IMR-32(Cat # L008V1 ).
Western blot analysis of MDH2 Antibody (Center) (AP17557PU-N) in K562 cell line and mouse heart tissue lysates (35ug/lane). MDH2 (arrow) was detected using the purified Pab.(2ug/ml) .
Recombinant human MDH2, 25-338aa, His-tagged
MDH2 antibody detects MDH2 protein by Western blot analysis. A. 50 ug rat lung lysate/extract. 10 % SDS-PAGE. MDH2 antibody (TA308153) dilution: 1:5000
Sample(30 ug of whole cell lysate). A:293T. 12% SDS PAGE. TA308153 diluted at 1:1000
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308153 diluted at 1:10000.
MDH2 antibody detects MDH2 protein at mitochondrion on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. MDH2 antibody (TA308153) dilution: 1:500.
MDH2 antibody detects MDH2 protein at mitochondrion on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MDH2 antibody (TA308153) dilution: 1:500.
MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. MDH2 antibody (TA308153) dilution: 1:500.
Anti-MDH2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308153 | MDH2 antibody |
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| Rabbit Polyclonal antibody to MDH2 (malate dehydrogenase 2, NAD (mitochondrial)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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| TA318626 | MDH2 antibody |
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| Mouse Anti-Human MDH2 Monoclonal Antibody | |||||||
 |
Mouse | IgG2a | 1G12 | Purified | Hu | E, P, WB |
50 µg /
€400.00
|
| OriGene Technologies, Inc. | |||||||
| AP17557PU-N | MDH2 antibody |
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 |
Rabbit | Hu, Ms | WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| R1099 | MDH2 antibody |
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|
Sheep | Serum | Por | E, WB |
2 ml /
€1,180.00
|
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| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201095BN | MDH2 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201095LP | MDH2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201095RB | MDH2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309906 | Mdh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309906BN | Mdh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309906LP | Mdh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309906RB | Mdh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301095 | MDH2 |
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| Recombinant protein of human malate dehydrogenase 2, NAD (mitochondrial) (MDH2), nuclear gene encoding mitochondrial protein | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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| AR09617PU-L | MDH2 (25-338, His-tag) |
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 |
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.25 mg /
€750.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
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| AR09617PU-N | MDH2 (25-338, His-tag) |
||||
|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
50 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
|||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC201095L2V | Lenti ORF particles, MDH2 (mGFP-tagged) - Human malate dehydrogenase 2, NAD (mitochondrial) (MDH2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC201095L1V | Lenti ORF particles, MDH2 (Myc-DDK tagged) - Human malate dehydrogenase 2, NAD (mitochondrial) (MDH2), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205049L2V | Lenti ORF particles, Mdh2 (GFP-tagged) - Mouse malate dehydrogenase 2, NAD (mitochondrial) (Mdh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR205049L1V | Lenti ORF particles, Mdh2 (Myc-DDK-tagged) - Mouse malate dehydrogenase 2, NAD (mitochondrial) (Mdh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211474L1V | Lenti ORF particles, Mdh2 (Myc-DDK-tagged ORF) - Rat malate dehydrogenase 2, NAD (mitochondrial) (Mdh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
