MDM2 antibody

Principal name

MDM2 antibody

Alternative names for MDM2 antibody

p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2, E3 ubiquitin-protein ligase Mdm2

SwissProt ID

P23804 (Mouse), Q00987 (Human)

Gene ID

4193 (MDM2), 17246 (Mdm2), 4250 (SCGB2A2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Fe (Feline), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Dot blot (Dot), ELISA (detection) (E(detection))

Background of MDM2 antibody

MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation. MDM2 binds to p53 and amplification of MDM2 in sarcomas leads to escape from p53-regulated growth control. This mechanism of tumorigenesis parallels that for virus-induced tumors in which viral oncogene products bind to and functionally inactivate p53. Over-expression of the MDM2 oncogene was found in leukemias. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. MDM2 interacts physically and functionally with the retinoblastoma (RB) protein and can inhibit its growth regulatory capacity. Both RB and p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2-p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. Four out of five of these alternatively spliced forms (MDM2a-MDMd) are missing substantial portions of the p53 binding domain and retain the acidic domain and the zinc-finger domains. The fifth and smallest transcript (MDM2e) retains the largest spliced region encoding the p53 binding domain; however, it lacks the nuclear localization signal, the acidic domain and zinc-finger domains. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full-length MDM2 transcript is expressed in normal tissue.
Cellular Localization: MDM2 is found in the nucleus and cytoplasm, however, it is expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleus. The nucleolar localization signals in both ARF and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.

General readings

General / background references:Prives C Signaling to p53: breaking the MDM2-p53 circuit. Cell 95:5-8 (1998). PubMed PMID: 9778240
Han J et al. Activation of the transcription factor MEF2C by the MAP kinase p38 in inflammation. Nature 386:296-9 (1997). PubMed PMID: 9069290
Fakharzadeh SS et al. Tumorigenic potential associated with enhanced expression of a gene that is amplified in a mouse tumor cell line. EMBO J 10:1565-9 (1991). PubMed PMID: 2026149

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MDM2 (C-term) antibody

Western blot analysis of MDM2 (arrow) using rabbit polyclonal Mdm2 Antibody (C-term) (Cat.#TA302211). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MDM2 rabbit polyclonal antibody. (TA590124) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MDM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801812) Mouse IgG1 OTI1E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MDM2 pSer166 antibody

Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using MDM2 (phospho-Ser166) Antibody Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

MDM2 pSer166 antibody

Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using MDM2 (phospho-Ser166) Antibody Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MDM2 antibody

Immunoperoxidase of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5 ug/ml] Mouse IgG1 1A7 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MDM2 antibody

TA302536 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Fe, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Western blot analysis of extracts from SKOV3 cells, using MDM2 antibody (#TA311995).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Western blot analysis of extracts from COS7 cells, using MDM2 (Ab-166) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM2 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MDM2 (177-195) antibody

WB using Anti-MDM2 (Rabbit) to detect a band (arrow) corresponding to mouse MDM2 protein present in mouse MEF cells (lane 2) but not human kidney HEK293 cells (lane 1). The primary antibody was diluted to 1:500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT. Rabbit IgG Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Western blot analysis of MDM2 expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Western blot analysis on COS7 cell lysate using MDM2 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

Western Blot: MDM2 Antibody [TA336703] - Detection of MDM2 in human liver protein using TA336703. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

MDM2 Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

ELISA: MDM2 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

ELISA: MDM2 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

ELISA: MDM2 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

MDM2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (RC219518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2. Mouse IgG1 OTI4B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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