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MeCP2 antibody

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Principal name

MeCP2 antibody

Alternative names for MeCP2 antibody

MeCP-2 protein, Methyl-CpG-binding protein 2

SwissProt ID

P51608 (Human), Q00566 (Rat), Q95LG8 (Macfa), Q9Z2D6 (Mouse)

Gene ID

4204 (MECP2), 17257 (Mecp2), 29386

Ncbi ID

9606, 10090, NP_004983, 9541, NP_001104262.1, NP_001104262, NM_010788, NM_001081979, NM_022673, NM_001110792

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), African clawed frog, Bov (Bovine), Eq (Equine), GP (Guinea Pig), Ye (Yeast), Por (Porcine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Dot blot (Dot)

Background of MeCP2 antibody

The epigenetic information encoded as methylated CpG dinucleotides to the transcriptional machinery is transferred by a multifunction methylated DNA binding protein called Methylated CpG biding protein 2. Mutation in MeCP2 protein led to debilitating neurogenerative X-linked autism-spectrum neurodevelopmental disease Rhett syndrome (RTT). Several functional domains have been identified on the MeCP2 protein including Methylated non-methylated DNA binding domains in the first 294 amino acids and a chromatin binding motif near the C-terminal DNA region of the protein (1). The genetic and epigenetic defects led to reduced expression of MeCP2 protein in brain of was autism patients, it may be an important factor in the complex etiology of this disease (2). The gene is located on Xq28 chromosome. Defects in the MeCP2 gene care caused by Angelman syndrome also known as happy puppet syndrome which a neuron-developmental disorder characterized by severe mental retardation, absent of speech, ataxia, sociable affects and dysmorphic facial features similar to Rhett syndrome clinical feature. Rett syndrome (RS) is an X-linked neuron-developmental disorder and the second most common cause of genetic mental retardation in females. Different mutations in MECP2 are found in up to 95% of typical cases of RS. The onset of this disease is caused by non progressive encephalopathy with neonatal onset. The defects in MeCP2 is cause of X-linked mental retardation, Autism 3 (AUTSX3) about 80% of the Rhett syndrome cases have mutational defects in the MeCP2 gene. Expression of this protein sprout generation of neuron and reversal of neurological defects in a moue model of Rett syndrome (3).
MeCP2 protein has 2 AT hook DNA biding domains, a transcriptional repression domain (TRD) and one MBD or Methyl CpG binding domain on the amino terminal end (1-96). Methyl CGp binding protein 2 gene is located on fragile X chromosome and this chromosomal protein binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. The binding is not influenced by sequences flanking the methyl-CpGs. Upon binding it mediates transcriptional repression through interaction with histone deacetylase and co repressor SIN3A. MeCP2, is responsible for transcriptional repression of the leukosialin gene by repression of the Sp1-activated trasnacription. Methyl-CpG-binding protein MeCP2 represses Sp1-activated transcription of the human leukosialin gene when the promoter is methylated (3).

General readings

1. Tatiana Nikitina,1 Xi Shi,2 Rajarshi P. Ghosh,2 Rachel A. Horowitz-Scherer,1 Jeffrey C. Hansen,3 and Christopher L. Woodcock. Multiple Modes of
Interaction between the Methylated DNA Binding Protein MeCP2 and Chromatin. Mol Cell Biol. 2007 November 13.10.1128/MCB.01593-06.
2. Raman P. Nagarajan, Amber R. Hogart, Ynnez Gwye, Michelle R. Martin, and Janine M. LaSalle. Reduced MeCP2 expression is frequent in autism frontal
cortex and correlates with aberrant MECP2 promoter methylation. Epigenetics. 2006 October; 1(4): 172–182.
3. Science 1143-1147, 2007
4. Kudo S. Mol Cell Biol. 1998 Sep;18(9):5492-9

H00004204-M01 - MeCP2
Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
H00004204-M01 - MeCP2
MECP2 monoclonal antibody (M01), clone 4B6 Western Blot analysis of MECP2 expression in MCF-7 ( Cat # L046V1 ).
H00004204-M01 - MeCP2
Western Blot analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.
H00004204-M01 - MeCP2
MECP2 monoclonal antibody (M01), clone 4B6. Western Blot analysis of MECP2 expression in NIH/3T3 ( Cat # L018V1 ).
H00004204-M01 - MeCP2
Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.
PAB0170 - MeCP2
Western blot analysis of MECP2 in HeLa cell lysate. Using MECP2 polyclonal antibody ( Cat # PAB0170 ) at 2 µg/ml.
PAB0646 - MeCP2
Western blot and Immunoprecipitation analysis using the Mecp2 polyclonal antibody ( Cat # PAB0646 ).Panel A. HEK 293T were transiently transfected with an expression vector for HA-tagged Mecp2. Whole cell extracts were immunoprecipitated with an antibody against the HA-tag ( positive control; 2 µg ) ( lane 1 ), with Mecp2 polyclonal antibody ( Cat # PAB0646 ) ( lane 2 ) and with beads only ( lane 3 ). The presence of HA-Mecp2 in the immunoprecipitates was demonstrated by western blot analysis with an antibody directed against HA.Panel B. Nuclear extracts from untransfected HeLa cells were immunoprecipitated with the Mecp2 polyclonal antibody ( Cat # PAB0646 ) ( 2 µg ) and beads only ( lanes 5 and 6 ). The presence of Mecp2 in the immunoprecipitates and in the unprecipitated nuclear extracts ( lane 4 ) was demonstrated by western blot analysis with the Mecp2 antibody ( 0.4 µg/ml ).
PAB0646 - MeCP2
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Mecp2 polyclonal antibody ( Cat # PAB0646 ), crude serum, and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be : 1 : 15,000.
PAB0646 - MeCP2
ChIP results obtained with the Mecp2 polyclonal antibody ( Cat # PAB0646 ) . Legend : ChIP assays were performed using human osteosarcoma ( U-2 OS ) cells.
H00004204-M03 - MeCP2
Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
H00004204-M03 - MeCP2
MECP2 monoclonal antibody (M03), clone 1B11. Western Blot analysis of MECP2 expression in Jurkat ( Cat # L017V1 ).
H00004204-M03 - MeCP2
MECP2 monoclonal antibody (M03), clone 1B11 Western Blot analysis of MECP2 expression in Hela NE ( Cat # L013V3 ).
H00004204-M03 - MeCP2
Detection limit for recombinant GST tagged MECP2 is approximately 0.1ng/ml as a capture antibody.
H00004204-B01 - MeCP2
Western Blot analysis of MECP2 expression in transfected 293T cell line (H00004204-T01) by MECP2 MaxPab polyclonal antibody.Lane 1: MECP2 transfected lysate(53.46 KDa).Lane 2: Non-transfected lysate.
H00004204-D01P - MeCP2
Western Blot analysis of MECP2 expression in transfected 293T cell line (H00004204-T01) by MECP2 MaxPab polyclonal antibody.Lane 1: MECP2 transfected lysate(52.40 KDa).Lane 2: Non-transfected lysate.
ARP43584_T100 - MeCP2
Human Jurkat; WB Suggested Anti-MECP2 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; MECP2 antibody - N-terminal region (ARP43584_T100) in Human Jurkat cells using Western Blot
AP12280PU-N - MeCP2
(LEFT) Western blot analysis of anti-MeCP2 Antibody (Cat.#AP12280PU-N) in Jurkat cell line lysates (35ug/lane). MeCP2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-MeCP2 Antibody (Cat.#AP12280PU-N) in cerebellum heart tissue lysates (35ug/lane). MeCP2 (arrow) was detected using the purified Pab.
AP13005PU-N - MeCP2
Dot blot analysis of anti-Phospho-MeCP2 pSer80 Antibody (Cat.#AP13005PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
AP08458PU-N - MeCP2
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
PAB14073 - MeCP2
Nuclear extracts of mouse fibroblasts ( NIH/3T3, 40 µg ) were analysed by Western blot using Mecp2 polyclonal antibody ( Cat # PAB14073 ) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
PAB14073 - MeCP2
ELISA was performed using a serial dilution of Mecp2 polyclonal antibody ( Cat # PAB14073 ).The wells were coated with the peptide used for immunisation of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 136,000.
ARP32694_P050 - MeCP2
Human HepG2; WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; MECP2 antibody - N-terminal region (ARP32694_P050) in Human HepG2 cells using Western Blot
ARP38539_P050 - MeCP2
Human HepG2; WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; MECP2 antibody - middle region (ARP38539_P050) in Human HepG2 cells using Western Blot
AM31107PU-N - MeCP2
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31107PU-N - MeCP2
MECP2 monoclonal antibody clone 4B6. Western Blot analysis of MECP2 expression in NIH/3T3.
AM31107PU-N - MeCP2
MECP2 monoclonal antibody clone 4B6 Western Blot analysis of MECP2 expression in MCF-7.
AM31107PU-N - MeCP2
MECP2 monoclonal antibody clone 4B6. Western Blot analysis of MECP2 expression in rat muscle.
AM31107PU-N - MeCP2
Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.
AP31635SU-N - MeCP2
Western blot of neonatal Rat brain showing specific immunolabeling of the ~ 75k MeCP2 protein phosphorylated at Ser80.
AP52648PU-N - MeCP2
Formalin fixed, paraffin embedded human brain tissue stained with MeCP2 Antibody (N-term) Cat.-No AP52648PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
AP52648PU-N - MeCP2
Western blot analysis of MeCP2 Antibody (N-term) Cat.-No AP52648PU-N in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).
AP52648PU-N - MeCP2
Flow cytometric analysis of MDA-MB435 cells using MeCP2 Antibody (N-term) Cat.-No AP52648PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
75-273 - MeCP2
Adult rat hippocampus immunohistochemistry
75-273 - MeCP2
Bottom right: serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N227/21 (left) and loading control (right) TC supe
73-273 - MeCP2
Adult rat hippocampus immunohistochemistry
73-273 - MeCP2
Bottom right: serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N227/21 (left) and loading control (right) TC supe
TA331114 - MeCP2
WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TA332083 - MeCP2
WB Suggested Anti-MECP2 Antibody Titration: 2.5ug/ml; Positive Control: Jurkat cell lysate
TA332847 - MeCP2
Western blot analysis of extracts of various cell lines, using MECP2 antibody.
TA343494 - MeCP2
WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate
TA347243 - MeCP2
WB using the antibody against MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
TA347243 - MeCP2
ChIP assays were performed using human osteosarcoma (U2OS) cells, the ab against MeCP2 and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 5 ug of ab were used per ChIP experiment. IgG (1 ug/IP) was negative IP control. qPCR was performed with primers for the promoters of the ZMYND10 gene (positive control) and CDC6 gene (negative control). Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR analysis).
TA347243 - MeCP2
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against MeCP2 and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the purified antibody was estimated to be: 1:32,900.
TA347244 - MeCP2
WB using the antibody against MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
TA347245 - MeCP2
WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2 , diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
TA347245 - MeCP2
Determination of the titer.To determine the titer, an ELISA was performed using a serial dilution of the antibody against human MeCP2 , in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000.
TA347246 - MeCP2
C15310106-1,WB using the antibody against mouse MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
TA347246 - MeCP2
C15310106-2,Determination of the titer. To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse MeCP2. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:136,000.
CPA9419-100ul - MeCP2
Western blot analysis of MeCP2 expression in Hela (A) whole cell lysates.
TA309165 - MECP2
Western blot of neonatal rat brain showing specific immunolabeling of the ~ 75k MeCP2 protein phosphorylated at Ser80.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MeCP2 (81-170) antibody
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4B6 Purified Hu, Ms, Rt E, P, WB
50 µg / €420.00
  Acris Antibodies GmbH
+4 additional images

Highly validated antibody!

MeCP2 antibody
  Rabbit Anti-MeCP2 (Ser80) Antibody (Phospho-Specific)  
Western blot of neonatal rat brain showing specific immunolabeling of the ~ 75k MeCP2 protein phosphorylated at Ser80. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €338.00
  OriGene Technologies, Inc.

MeCP2 antibody
  Rabbit Polyclonal Anti-MECP2 Antibody  
WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye WB
50 µg / €360.00
  OriGene Technologies, Inc.

MeCP2 antibody
  Rabbit Polyclonal Anti-MECP2 Antibody  
WB Suggested Anti-MECP2 Antibody Titration: 2.5ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

MeCP2 antibody
  Rabbit anti-MECP2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MECP2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

MeCP2 antibody
  Rabbit Polyclonal Anti-MECP2 Antibody  
WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €360.00
  OriGene Technologies, Inc.

MeCP2 antibody
  Rabbit Polyclonal MeCP2 Antibody  
WB using the antibody against MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €392.00
  OriGene Technologies, Inc.
+2 additional images

MeCP2 antibody
  Rabbit Polyclonal MeCP2 Antibody  
WB using the antibody against MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms
50 µg / €392.00
  OriGene Technologies, Inc.

MeCP2 antibody
  Rabbit Polyclonal MeCP2 Antibody  
WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2 , diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu E
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MeCP2 antibody
  Rabbit Polyclonal MeCP2 Antibody  
C15310106-1,WB using the antibody against mouse MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Ms E
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MeCP2 antibody
Adult rat hippocampus immunohistochemistry Mouse IgG1 N227/21 Supernatant Hu, Ms, Rt C, Dot
5 ml / €460.00
  Antibodies Incorporated
+1 additional image

NeuroMab

MeCP2 antibody
Adult rat hippocampus immunohistochemistry Mouse IgG1 N227/21 Purified Hu, Ms, Rt C, Dot
0.1 ml / €540.00
  Antibodies Incorporated
+1 additional image

NeuroMab

MeCP2 (1-25, 181-195) antibody
Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €380.00
  Acris Antibodies GmbH

MeCP2 antibody
(LEFT) Western blot analysis of anti-MeCP2 Antibody (Cat.#AP12280PU-N) in Jurkat cell line lysates (35ug/lane). MeCP2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-MeCP2 Antibody (Cat.#AP12280PU-N) in cerebellum heart tissue lysates (35ug/lane). MeCP2 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MeCP2 pSer80 antibody
Dot blot analysis of anti-Phospho-MeCP2 pSer80 Antibody (Cat.#AP13005PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

MeCP2 (N-term) antibody
Formalin fixed, paraffin embedded human brain tissue stained with MeCP2 Antibody (N-term) Cat.-No AP52648PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH
+2 additional images

CRISPR

Catalog No. Species Donor Vector  

MECP2 - human gene knockout kit via CRISPR
MECP2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MECP2 - human gene knockout kit via CRISPR
MECP2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MECP2 - human gene knockout kit via CRISPR
MECP2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MECP2 - human gene knockout kit via CRISPR
MECP2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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