MeCP2 antibody

Principal name

MeCP2 antibody

Alternative names for MeCP2 antibody

MeCP-2 protein, Methyl-CpG-binding protein 2

SwissProt ID

P51608 (Human), Q00566 (Rat), Q95LG8 (Macfa), Q9Z2D6 (Mouse)

Gene ID

4204 (MECP2), 17257 (Mecp2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), African clawed frog, Bov (Bovine), Eq (Equine), GP (Guinea Pig), Ye (Yeast), Por (Porcine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ChIP assay (ChIP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Dot blot (Dot)

Background of MeCP2 antibody

The epigenetic information encoded as methylated CpG dinucleotides to the transcriptional machinery is transferred by a multifunction methylated DNA binding protein called Methylated CpG biding protein 2. Mutation in MeCP2 protein led to debilitating neurogenerative X-linked autism-spectrum neurodevelopmental disease Rhett syndrome (RTT). Several functional domains have been identified on the MeCP2 protein including Methylated non-methylated DNA binding domains in the first 294 amino acids and a chromatin binding motif near the C-terminal DNA region of the protein (1). The genetic and epigenetic defects led to reduced expression of MeCP2 protein in brain of was autism patients, it may be an important factor in the complex etiology of this disease (2). The gene is located on Xq28 chromosome. Defects in the MeCP2 gene care caused by Angelman syndrome also known as happy puppet syndrome which a neuron-developmental disorder characterized by severe mental retardation, absent of speech, ataxia, sociable affects and dysmorphic facial features similar to Rhett syndrome clinical feature. Rett syndrome (RS) is an X-linked neuron-developmental disorder and the second most common cause of genetic mental retardation in females. Different mutations in MECP2 are found in up to 95% of typical cases of RS. The onset of this disease is caused by non progressive encephalopathy with neonatal onset. The defects in MeCP2 is cause of X-linked mental retardation, Autism 3 (AUTSX3) about 80% of the Rhett syndrome cases have mutational defects in the MeCP2 gene. Expression of this protein sprout generation of neuron and reversal of neurological defects in a moue model of Rett syndrome (3).
MeCP2 protein has 2 AT hook DNA biding domains, a transcriptional repression domain (TRD) and one MBD or Methyl CpG binding domain on the amino terminal end (1-96). Methyl CGp binding protein 2 gene is located on fragile X chromosome and this chromosomal protein binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. The binding is not influenced by sequences flanking the methyl-CpGs. Upon binding it mediates transcriptional repression through interaction with histone deacetylase and co repressor SIN3A. MeCP2, is responsible for transcriptional repression of the leukosialin gene by repression of the Sp1-activated trasnacription. Methyl-CpG-binding protein MeCP2 represses Sp1-activated transcription of the human leukosialin gene when the promoter is methylated (3).

General readings

1. Tatiana Nikitina,1 Xi Shi,2 Rajarshi P. Ghosh,2 Rachel A. Horowitz-Scherer,1 Jeffrey C. Hansen,3 and Christopher L. Woodcock. Multiple Modes of
Interaction between the Methylated DNA Binding Protein MeCP2 and Chromatin. Mol Cell Biol. 2007 November 13.10.1128/MCB.01593-06.
2. Raman P. Nagarajan, Amber R. Hogart, Ynnez Gwye, Michelle R. Martin, and Janine M. LaSalle. Reduced MeCP2 expression is frequent in autism frontal
cortex and correlates with aberrant MECP2 promoter methylation. Epigenetics. 2006 October; 1(4): 172–182.
3. Science 1143-1147, 2007
4. Kudo S. Mol Cell Biol. 1998 Sep;18(9):5492-9

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MeCP2 (81-170) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4B6 Purified Hu, Ms, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MeCP2 antibody

Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml] Mouse IgG2a 4B6 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MeCP2 antibody

Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml] Mouse IgG2a 1B11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MeCP2 antibody

WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

WB Suggested Anti-MECP2 Antibody Titration: 2.5ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

Western blot analysis of extracts of various cell lines, using MECP2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

WB Suggested Anti-MECP2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

WB using the antibody against MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

WB using the antibody against MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms
50 µg / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2 , diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

C15310106-1,WB using the antibody against mouse MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

MeCP2 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG1 N227/21 Supernatant Hu, Ms, Rt C, Dot
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

MeCP2 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG1 N227/21 Purified Hu, Ms, Rt C, Dot
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

MeCP2 (1-25, 181-195) antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MeCP2 Control Peptide

MeCP2
0.25 mg / €250.00
  Acris Antibodies GmbH

MeCP2 (469-486) antibody

MeCP2 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

MeCP2 antibody

(LEFT) Western blot analysis of anti-MeCP2 Antibody (Cat.#AP12280PU-N) in Jurkat cell line lysates (35ug/lane). MeCP2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-MeCP2 Antibody (Cat.#AP12280PU-N) in cerebellum heart tissue lysates (35ug/lane). MeCP2 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MeCP2 pSer80 antibody

Dot blot analysis of anti-Phospho-MeCP2 pSer80 Antibody (Cat.#AP13005PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

MeCP2 pSer80 antibody

Western blot of neonatal Rat brain showing specific immunolabeling of the ~ 75k MeCP2 protein phosphorylated at Ser80. Rabbit Serum Hu, Ms, Rt C, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

MeCP2 (N-term) antibody

Formalin fixed, paraffin embedded human brain tissue stained with MeCP2 Antibody (N-term) Cat.-No AP52648PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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