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MED1 antibody

Principal name

MED1 antibody

Alternative names for MED1 antibody

ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, Mediator complex subunit 1, Peroxisome proliferator-activated receptor-binding protein, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, p53 regulatory protein RB18A, Vitamin D receptor-interacting protein complex component DRIP205, Activator-recruited cofactor 205 kDa component

SwissProt ID

Q15648 (Human), Q6INP8 (Xenla), Q8VDQ7 (Mouse), Q925J9 (Mouse)

Gene ID

5469 (MED1), 19014 (Med1)

Ncbi ID

9606, NP_004765.2, NM_004774, NP_001073587.1, NM_134027, NM_001080118, NM_013634

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ChIP assay (ChIP)

Background of MED1 antibody

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the liganddependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

H00005469-M01

Western Blot analysis of PPARBP expression in transfected 293T cell line by PPARBP monoclonal antibody (M01), clone 2A2.Lane 1: PPARBP transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.

H00005469-M01

Detection limit for recombinant GST tagged PPARBP is approximately 0.3ng/ml as a capture antibody.

H00005469-M01

Detection limit for recombinant GST tagged PPARBP is approximately 0.1ng/ml as a capture antibody.

H00005469-M02

Detection limit for recombinant GST tagged PPARBP is approximately 0.1ng/ml as a capture antibody.

H00005469-M03

Detection limit for recombinant GST tagged PPARBP is approximately 0.1ng/ml as a capture antibody.

H00005469-M04

Detection limit for recombinant GST tagged PPARBP is approximately 0.3ng/ml as a capture antibody.

H00005469-M05

Detection limit for recombinant GST tagged PPARBP is approximately 0.03ng/ml as a capture antibody.

H00005469-M06

Immunohistochemistry analysis of TRAP220 antibody (Cat.-No.: AP06737PU-N) in paraffin-embedded human breast carcinoma tissue.

AP06737PU-N

Western blot analysis of TRAP220 antibody (Cat.-No.: AP06737PU-N) in extracts from Jurkat cells.

AP06737PU-N

Immunohistochemistry analysis of paraffin-embedded human heart, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HuvEc cells treated with Serum 20% 30', using PPAR-BP (Phospho-Thr1457) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using PPAR-BP (Ab-1457) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using MED1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of TRAP220 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of TRAP220 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of TRAP220 expression in HeLa (A); HuvEc (B); Jurkat (C) whole cell lysates.

Western blot analysis of TRAP220 (pT1457) expression in Jurkat (A); HeLa (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of TRAP220 (pT1457) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of TRAP220 (pT1457) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of extracts from Jurkat cells, using MED1 antibody.The lane on the right is treated with the synthesized peptide.

TA314366

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MED1 antibody.The picture on the right is treated with the synthesized peptide.

TA314366

Western blot analysis of PPAR-BP expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325682

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA314366	MED1 antibody
	Rabbit polyclonal anti-MED1 antibody
	Rabbit	IgG	Purified	Hu, Ms	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA325682	MED1 antibody
	Rabbit Polyclonal PPAR-BP Antibody
	Rabbit	IgG	Purified	Hu, Ms	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
AP06737PU-N	MED1 antibody
	Rabbit		Aff - Purified	Hu, Ms	P, WB	0.1 mg / €350.00
						OriGene Technologies GmbH
+1 additional image
A0556-1	MED1 pThr1457 antibody
	Rabbit		Aff - Purified	Hu, Ms	E, ICC/IF, P, WB	50 µg / €230.00
						Assay Biotech
+2 additional images
A0556-2	MED1 pThr1457 antibody
	Rabbit		Aff - Purified	Hu, Ms	E, ICC/IF, P, WB	0.1 mg / €330.00
						Assay Biotech
+2 additional images
B0556-1	MED1 antibody
	Rabbit		Aff - Purified	Hu, Ms	E, ICC/IF	50 µg / €230.00
						Assay Biotech
B0556-2	MED1 antibody
	Rabbit		Aff - Purified	Hu, Ms	E, ICC/IF	0.1 mg / €330.00
						Assay Biotech
C11093-1	MED1 antibody
	Rabbit		Aff - Purified	Hu, Ms	E, P, WB	50 µg / €230.00
						Assay Biotech
+1 additional image
C11093-2	MED1 antibody
	Rabbit		Aff - Purified	Hu, Ms	E, P, WB	0.1 mg / €330.00
						Assay Biotech
+1 additional image

Lysates

Catalog No.
LY417755	MED1 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC222118L2V	Lenti ORF particles, MED1 (mGFP-tagged) - Human mediator complex subunit 1 (MED1), 200 uL, >10^7 TU/mL
		200 µl / €1,360.00
		OriGene Technologies, Inc.

RC222118L1V	Lenti ORF particles, MED1 (Myc-DDK tagged) - Human mediator complex subunit 1 (MED1) , 200 uL, >10^7 TU/mL
		200 µl / €1,216.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR222482L2V	Lenti ORF particles, Med1 (GFP-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,586.00
		OriGene Technologies, Inc.

MR222479L2V	Lenti ORF particles, Med1 (GFP-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,226.00
		OriGene Technologies, Inc.

MR222481L2V	Lenti ORF particles, Med1 (GFP-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €2,617.00
		OriGene Technologies, Inc.

MR222482L1V	Lenti ORF particles, Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €2,339.00
		OriGene Technologies, Inc.

MR222479L1V	Lenti ORF particles, Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,103.00
		OriGene Technologies, Inc.

MR222481L1V	Lenti ORF particles, Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 3, 200 uL, >10^7 TU/mL
		200 µl / €2,359.00
		OriGene Technologies, Inc.

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MED1 antibody

Principal name

Alternative names for MED1 antibody

SwissProt ID

Gene ID

Ncbi ID

Available reactivities

Available hosts

Available applications