MED16 antibody
Principal name
MED16 antibody
Alternative names for MED16 antibody
DRIP92, THRAP5, Mediator complex subunit 16, Mediator of RNA polymerase II transcription subunit 16, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP92
Gene ID
10025 (MED16)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of MED16 antibody
- Kit Component:
- - KN218942G1, MED16 gRNA vector 1 in pCas-Guide vector
- KN218942G2, MED16 gRNA vector 2 in pCas-Guide vector
- KN218942D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Wang, Q., et al. J. Biol. Chem. 277(45):42852-42858(2002)
Kang, Y.K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(5):2642-2647(2002)
Suzuki, Y., et al. Genome Res. 11(5):677-684(2001)
Human Heart; THRAP5 antibody - C-terminal region (ARP32301_T100) in Human Heart cells using Immunohistochemistry
Human 293T; WB Suggested Anti-THRAP5 Antibody Titration: 0.12ug/ml. Positive Control: 293T cell lysate. There is BioGPS gene expression data showing that MED16 is expressed in HEK293T.; THRAP5 antibody - C-terminal region (ARP32301_T100) in Human 293T cells using Western Blot
Immunofluorescence of monoclonal antibody to THRAP5 on HeLa cell. [antibody concentration 10 ug/ml]
THRAP5 monoclonal antibody (M02), clone 2B7 Western Blot analysis of THRAP5 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged THRAP5 is approximately 1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to THRAP5 on HeLa cell. [antibody concentration 10 ug/ml]
THRAP5 monoclonal antibody (M03), clone 3B10 Western Blot analysis of THRAP5 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged THRAP5 is approximately 3ng/ml as a capture antibody.
THRAP5 monoclonal antibody (M04), clone 3A3 Western Blot analysis of THRAP5 expression in Hela NE( Cat # L013V3 ).
Human 721_B; WB Suggested Anti-MED16 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. MED16 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; MED16 antibody - N-terminal region (ARP31452_P050) in Human 721_B cells using Western Blot
Human Placenta; WB Suggested Anti-MED16 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; MED16 antibody - C-terminal region (ARP57927_P050) in Human Placenta cells using Western Blot
Western blot analysis of MED16 Antibody (Center) Cat.-No AP52650PU-N in 293 cell line lysates (35ug/lane). This demonstrates the MED16 antibody detected the MED16 protein (arrow).
WB Suggested Anti-THRAP5 Antibody Titration: 0.12ug/ml; Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that MED16 is expressed in HEK293T
Human Heart
Host: Rabbit; Target Name: MED16; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-MED16 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hepg2 and hela cell, Primary antibody: (MED16 Antibody) at dilution 1/400 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (MED16 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MED16 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
WB Suggested Anti-MED16 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateMED16 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA329291 | MED16 antibody |
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| Rabbit Polyclonal anti-MED16 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA330239 | MED16 antibody |
||||||
| Rabbit Polyclonal Anti-THRAP5 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | P, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA344538 | MED16 antibody |
||||||
| Rabbit Polyclonal Anti-MED16 Antibody - middle region | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344539 | MED16 antibody |
||||||
| Rabbit Polyclonal Anti-MED16 Antibody - C-terminal region | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Por, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350495 | MED16 antibody |
||||||
| Rabbit Polyclonal Anti-MED16 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP52650PU-N | MED16 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN218942 | MED16 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN218942BN | MED16 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN218942LP | MED16 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN218942RB | MED16 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
