MED25 antibody

Principal name

MED25 antibody

Alternative names for MED25 antibody

ACID1, ARC92, PTOV2, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78, Mediator of RNA polymerase II transcription subunit 25

SwissProt ID

A2VE44, A4IHD9, Q6PEH8, Q71SY5, Q8VCB2

Gene ID

81857 (MED25), 75613 (Med25)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), GP (Guinea Pig), Por (Porcine), Rt (Rat), Ze (Zebrafish), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP)

Background of MED25 antibody

Kit Component:
- KN309938G1, Med25 gRNA vector 1 in pCas-Guide vector
- KN309938G2, Med25 gRNA vector 2 in pCas-Guide vector
- KN309938D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Han EH et al. PLoS One. 2012;7(8):e44007
Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010)
Leal, A., et al. Neurogenetics 10(4):275-287(2009)
Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MED25 antibody

  Rabbit Polyclonal Anti-MED25 Antibody  
WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Ze ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED25 antibody

  Rabbit Polyclonal Anti-MED25 Antibody  
Host: Rabbit; Target Name: MED25; Sample Tissue: OVCAR-3 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED25 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with MED25 Antibody (N-term) Cat.-No AP52653PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

MED25 - human gene knockout kit via CRISPR

MED25 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MED25 - human gene knockout kit via CRISPR

MED25 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MED25 - human gene knockout kit via CRISPR

MED25 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MED25 - human gene knockout kit via CRISPR

MED25 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Med25 - mouse gene knockout kit via CRISPR

Med25 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Med25 - mouse gene knockout kit via CRISPR

Med25 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Med25 - mouse gene knockout kit via CRISPR

Med25 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Med25 - mouse gene knockout kit via CRISPR

Med25 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MED25

  Recombinant protein of human mediator complex subunit 25 (MED25)  
MED25 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MED25 overexpression lysate

MED25 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MED25 (mGFP-tagged) - Human mediator complex subunit 25 (MED25), 200 uL, >10^7 TU/mL

Lenti ORF particles, MED25 (mGFP-tagged) - Human mediator complex subunit 25 (MED25), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, MED25 (Myc-DDK tagged) - Human mediator complex subunit 25 (MED25) , 200 uL, >10^7 TU/mL

Lenti ORF particles, MED25 (Myc-DDK tagged) - Human mediator complex subunit 25 (MED25) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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