MED25 antibody

Principal name

MED25 antibody

Alternative names for MED25 antibody

ACID1, ARC92, PTOV2, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78, Mediator of RNA polymerase II transcription subunit 25

Gene ID

81857 (MED25)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), GP (Guinea Pig), Por (Porcine), Rt (Rat), Ze (Zebrafish), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Frozen Sections (C)

Background of MED25 antibody

This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].

General readings

Han EH et al. PLoS One. 2012;7(8):e44007
Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010)
Leal, A., et al. Neurogenetics 10(4):275-287(2009)
Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MED25 antibody

WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Ze ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED25 antibody

Host: Rabbit; Target Name: MED25; Sample Tissue: OVCAR-3 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED25 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with MED25 Antibody (N-term) Cat.-No AP52653PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MED25 antibody

MED25 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

MED25 (N-term) antibody

MED25 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MED25

MED25 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MED25 overexpression lysate

MED25 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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