MED27 antibody

Principal name

MED27 antibody

Alternative names for MED27 antibody

CRSP34, CRSP8, Mediator complex 27, CRSP complex subunit 8, Mediator of RNA polymerase II transcription subunit 27, Cofactor required for Sp1 transcriptional activation subunit 8

SwissProt ID

Q2TBN7 (Bovin), Q6P2C8 (Human), Q6Q7J5 (Pig), Q9DB40 (Mouse)

Gene ID

9442 (MED27)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MED27 antibody

he activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP8 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

General readings

Malik S., et al. (2000) Mol. Cell. 5:753-760Ryu S., et al. (1999) Nature. 397:446-450

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MED27 antibody

WB Suggested Anti-MED27 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED27 antibody

Human Brain; WB Suggested Anti-MED27 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; MED27 antibody - middle region (ARP33743_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MED27 antibody

Western blot analysis of extracts from HeLa cells, using MED27 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MED27 antibody

WB Suggested Anti-CRSP8 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MED27 antibody

Western Blot analysis of MED27 expression in transfected 293T cell line (H00009442-T01) by MED27 MaxPab polyclonal antibody.

Lane 1: CRSP8 transfected lysate(34.21 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MED27 antibody

Western Blot analysis of MED27 expression in transfected 293T cell line (H00009442-T01) by MED27 MaxPab polyclonal antibody.

Lane 1: CRSP8 transfected lysate(34.21 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MED27 antibody

MED27 MaxPab rabbit polyclonal antibody. Western Blot analysis of MED27 expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MED27 antibody

Immunoperoxidase of monoclonal antibody to CRSP8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 0.3 ug/ml] Mouse IgG1 8B8 Purified Hu E, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MED27 antibody

Human HepG2; WB Suggested Anti-CRSP8 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; CRSP8 antibody - C-terminal region (ARP38444_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

MED27 antibody

Immunohistochemistry-Paraffin: MED27 Antibody [NBP1-84377] - Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MED27 (1-311, His-tag)

MED27 Human Purified > 80 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

MED27 (1-311, His-tag)

MED27 Human Purified > 80 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

MED27

MED27 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MED27

MED27 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CRSP8 293T Cell Transient Overexpression Lysate(Denatured)

CRSP8 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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