MED6 antibody

Principal name

MED6 antibody

Alternative names for MED6 antibody

Mediator complex 6, ARC33, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, Renal carcinoma antigen NY-REN-28

SwissProt ID

O75586 (Human), P38782 (Yeast), Q3SZY9 (Bovin), Q921D4 (Mouse)

Gene ID

10001 (MED6)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), EPair for Elisa (EPair)

Background of MED6 antibody

MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

General readings

Pavri, R., et al. Mol. Cell 18(1):83-96(2005)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(50):49671-49674(2003)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MED6 antibody

Hum. Fetal Brain; Host:Rabbit. Target Name:MED6. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; MED6 antibody - middle region (ARP34220_P050) in Hum. Fetal Brain cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MED6 antibody

WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED6 antibody

Host:Rabbit; Target Name:MED6; Sample Tissue:Human Fetal Brain; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED6 antibody

Host: Rabbit; Target Name: MED6; Sample Tissue: Esophagus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED6 antibody

Host: Rabbit; Target Name: MED6; Sample Tissue: Lung Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED6 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED6 (RC202504, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED6.(1:2000) Mouse IgG1 OTI3C9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MED6 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED6 (RC202504, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED6.(1:2000) Mouse IgG2a OTI3B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MED6 (Center) antibody

Western blot analysis of MED6 Antibody (Center) Cat.-No AP52657PU-N in WiDr cell line lysates (35ug/lane).This demonstrates the MED6 antibody detected the MED6 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MED6 (C-term) antibody

Western blot analysis of MED6 Antibody (C-term) Cat.-No AP52658PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the MED6 antibody detected the MED6 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MED6 antibody

Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T01) by MED6 MaxPab polyclonal antibody.

Lane 1: MED6 transfected lysate(27.17 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MED6 antibody

Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T02) by MED6 MaxPab polyclonal antibody.

Lane 1: MED6 transfected lysate(27.06 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MED6 antibody

Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T02) by MED6 MaxPab polyclonal antibody.

Lane 1: MED6 transfected lysate(27.06 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MED6 antibody

MED6 MaxPab rabbit polyclonal antibody. Western Blot analysis of MED6 expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MED6 antibody

Immunoperoxidase of monoclonal antibody to MED6 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] Mouse IgG2b 4C2 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MED6 antibody

Transfected 293T; WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Transfected 293T; MED6 antibody - middle region (ARP34219_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MED6 (full length, N-term HIS tag)

MED6 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

MED6

MED6 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

MED6 (Human) Matched Antibody Pair

MED6 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

MED6 (Human) Matched Antibody Pair

MED6 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

MED6 (Human) Matched Antibody Pair

MED6 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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