MED7 antibody

Principal name

MED7 antibody

Alternative names for MED7 antibody

ARC34, CRSP9, Mediator complex subunit 7, Mediator of RNA polymerase II transcription subunit 7, Cofactor required for Sp1 transcriptional activation subunit 9, CRSP complex subunit 9, Transcriptional coactivator CRSP33, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Activator-recruited cofactor 34 kDa component

SwissProt ID

O43513 (Human), Q2F7Z4 (Pig), Q3T123 (Bovin), Q9CZB6 (Mouse)

Gene ID

9443 (MED7)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P)

Background of MED7 antibody

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase (1). These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes, and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34) (2,3). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors (4). Med6 and Med7, as well as other components of SMCC, associate with coactivator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation (4,5). Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity (6).

General readings

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Ryu, S., et al. Proc. Natl. Acad. Sci. U.S.A. 96(13):7137-7142(1999)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MED7 antibody

Western blot analysis of CRSP9 over-expressed 293 cell line, cotransfected with CRSP9 Validated Chimera RNAi ( Cat # H00009443-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRSP9 monoclonal antibody (M01), clone 3E7 (Cat # H00009443-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2b 3E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MED7 antibody

WB Suggested Anti-CRSP9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateMED7 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED7 antibody

WB Suggested Anti-MED7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED7 antibody

WB Suggested Anti-MED7 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateMED7 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED7 (Center) antibody

Western blot analysis of MED7 Antibody (Center) Cat.-No AP52659PU-N in Hela cell line lysates (35ug/lane). This demonstrates the MED7 antibody detected the MED7 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MED7 antibody

Western Blot analysis of MED7 expression in transfected 293T cell line (H00009443-T01) by MED7 MaxPab polyclonal antibody.

Lane 1: CRSP9 transfected lysate(25.63 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MED7 antibody

Western Blot analysis of MED7 expression in transfected 293T cell line (H00009443-T01) by MED7 MaxPab polyclonal antibody.

Lane 1: CRSP9 transfected lysate(25.63 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MED7 antibody

CRSP9 monoclonal antibody (M02), clone 2D7. Western Blot analysis of CRSP9 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG2b 2D7 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MED7 antibody

Human Adult Liver; Rabbit Anti-CRSP9 Antibody . Catalog Number: ARP33741_P050. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver . Observed Staining: Nuclear (strong) in hepatocytes, strong signal, wide tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 – 2.0 sec. Protocol located in Reviews and Data.; CRSP9 antibody - N-terminal region (ARP33741_P050) in Human Adult Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

MED7 antibody

MED7 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MED7 (Center) antibody

Human Jurkat; WB Suggested Anti-MED7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. MED7 is supported by BioGPS gene expression data to be expressed in Jurkat.; MED7 antibody - middle region (ARP57925_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MED7 (transcript variant 2)

MED7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MED7 (1-233, His-tag)

MED7 Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

MED7 (1-233, His-tag)

MED7 Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

MED7

MED7 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MED7

MED7 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CRSP9 293T Cell Transient Overexpression Lysate(Denatured)

CRSP9 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

17 Item(s)

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