MED8 antibody

Principal name

MED8 antibody

Alternative names for MED8 antibody

Mediator complex 8, Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component

SwissProt ID

P38304 (Yeast), Q96G25 (Human), Q9D7W5 (Mouse)

Gene ID

112950 (MED8), 80509 (Med8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of MED8 antibody

Kit Component:
- KN309948G1, Med8 gRNA vector 1 in pCas-Guide vector
- KN309948G2, Med8 gRNA vector 2 in pCas-Guide vector
- KN309948D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Leonard, V.H., et al. J. Virol. 80(19):9667-9675(2006)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(50):49671-49674(2003)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MED8 antibody

Western blot analysis of extracts from Jurkat/K562 cells, using MED8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MED8 antibody

WB Suggested Anti-MED8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MED8 (Center) antibody

Western blot analysis of MED8 Antibody (Center) Cat.-No AP52660PU-N in HL-60 cell line lysates (35ug/lane). This demonstrates the MED8 antibody detected the MED8 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MED8 antibody

MED8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MED8 antibody

Western Blot analysis of MED8 expression in transfected 293T cell line (H00112950-T01) by MED8 MaxPab polyclonal antibody.

Lane 1: MED8 transfected lysate(23.32 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MED8 antibody

Detection limit for recombinant GST tagged MED8 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1D3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MED8 antibody

MED8 Mouse IgG2a 1E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MED8 antibody

Human Stomach; WB Suggested Anti-MED8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Stomach; MED8 antibody - N-terminal region (ARP52440_P050) in Human Stomach cells using Western Blot Rabbit Aff - Purified African clawed frog, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

MED8 - human gene knockout kit via CRISPR

MED8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MED8 - human gene knockout kit via CRISPR

MED8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MED8 - human gene knockout kit via CRISPR

MED8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MED8 - human gene knockout kit via CRISPR

MED8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MED8 (transcript variant 1)

MED8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MED8

MED8 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

MED8

MED8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MED8

MED8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MED8 293T Cell Transient Overexpression Lysate(Denatured)

MED8 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

MED8 overexpression lysate

MED8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MED8 overexpression lysate

MED8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MED8 (mGFP-tagged) - Human mediator complex subunit 8 (MED8), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, MED8 (mGFP-tagged) - Human mediator complex subunit 8 (MED8), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

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