MEKK5 / ASK1 antibody

Principal name

MEKK5 / ASK1 antibody

Alternative names for MEKK5 / ASK1 antibody

MAPK/ERK kinase kinase 5, MAPKKK5, MAP3K5, Apoptosis signal-regulating kinase 1, Mitogen-activated protein kinase kinase kinase 5

SwissProt ID

O35099 (Mouse), Q99683 (Human)

Gene ID

4217 (MAP3K5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of MEKK5 / ASK1 antibody

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MEKK5 / ASK1 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining. Mouse IgG1 2E4 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MEKK5 / ASK1 (C-term) antibody

Human Heart (formalin-fixed, paraffin-embedded) stained with MAP3K5 antibody AP22866PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MEKK5 / ASK1 antibody

Immunofluorescence of monoclonal antibody to MAP3K5 on A-431 cell. [antibody concentration 10 ug/ml] Mouse IgG1 2D11 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MEKK5 / ASK1 antibody

Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody (Cat.#TA302148).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody (Cat.#TA302149).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 (C-term) antibody

Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 (N-term) antibody

Western blot analysis of ASK1 in 3T3 cell lysate with ASK1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

Predicted band size: 155 kDa . Positive control: MDA-MB- 435 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: MDA-MB- 435 cells lysate Lane2: Treated with the peptide Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human colon and lung cancer tissue using MAP3K5 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

Western blot analysis of ASK1 expression in MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

Western blot analysis of ASK1 expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MEKK5 / ASK1 antibody

MEKK5 / ASK1 Rabbit Aff - Purified Hu WB
0.1 mg / €390.00
  Acris Antibodies GmbH

MEKK5 / ASK1 pSer83 antibody

MEKK5 / ASK1 Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MEKK5 / ASK1 pSer966 antibody

Immunohistochemistry analysis of ASK1 antibody (pSer966) Cat.-No AP01527PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MEKK5 / ASK1 pSer83 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (phospho-Ser83) antibody AP02455PU. Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

MEKK5 / ASK1 pSer83 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (phospho-Ser83) antibody AP02455PU. Rabbit Aff - Purified Hu P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MEKK5 / ASK1 pSer966 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 antibody (phospho-Ser966) (#AP02456PU) (Left) or the same antibody preincubated with Blocking peptide (Right). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

MEKK5 / ASK1 pSer966 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 antibody (phospho-Ser966) (#AP02456PU) (Left) or the same antibody preincubated with Blocking peptide (Right). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MEKK5 / ASK1 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using ASK1 antibody AP02654PU. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

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