Melanoma antibody

Principal name

Melanoma antibody

Alternative names for Melanoma antibody

Melanoma marker

Available reactivities

Hu (Human)

Available hosts

Mouse

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Melanoma antibody

The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. The MART-1/Melan A recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45 and the MART-1 (cocktail) and Tyrosinase make this quadruple antibody cocktail a first-order pan melanoma screener.

General readings

General / background references:Parker DC Improved Detection of Melanoma Metastases in Sentinel Lymph Nodes. US and Canadian Academy of Pathol Annual Meeting Abstra 406:71A (2001).Orchard GE Comparison of immunohistochemical labelling of melanocyte differentiation antibodies melan-A, tyrosinase and HMB 45 with NKIC3 and S100 protein in the evaluation of benign naevi and malignant melanoma. Histochem J 32:475-81 (2000). PubMed PMID: 11095072
Blessing K et al. Comparison of immunohistochemical staining of the novel antibody melan-A with S100 protein and HMB-45 in malignant melanoma and melanoma variants. Histopathology 32:139-46 (1998). PubMed PMID: 9543670

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Melanoma (25-110 kDa) antibody

Melanoma Mouse IgG1 NKI/C3 Ascites Hu P
1 ml / €220.00
  Acris Antibodies GmbH

Melanoma (150, 90kDa) antibody

Melanoma Mouse IgG1 NKI-M7 Supernatant Hu C
1 ml / €240.00
  Acris Antibodies GmbH

Melanoma (250, >450kDa) antibody

Melanoma Mouse IgG1 NKI-M6 Purified Hu C, ICC/IF
1 ml / €240.00
  Acris Antibodies GmbH

Melanoma antibody

Formalin fixed paraffin embedded human melanoma stained with Melanoma antibody DM079/DM079-05 Mouse IgG1 HMB 45 Supernatant Hu C, P
1 ml / €530.00
  Acris Antibodies GmbH

Melanoma antibody

Formalin fixed paraffin embedded human melanoma stained with Melanoma antibody DM079/DM079-05 Mouse IgG1 HMB 45 Supernatant Hu C, P
0.5 ml / €360.00
  Acris Antibodies GmbH

Melanoma (pan) antibody

Formalin Fixed Paraffin Embedded Human lymph node stained with Pan Melanoma Cocktail Antibody Cat.-No DM428 Mouse cocktail cocktail Supernatant Hu C, P
1 ml / €910.00
  Acris Antibodies GmbH

Melanoma (pan) antibody

Formalin Fixed Paraffin Embedded Human lymph node stained with Pan Melanoma Cocktail Antibody Cat.-No DM428 Mouse cocktail cocktail Supernatant Hu C, P
0.5 ml / €580.00
  Acris Antibodies GmbH

Melanoma (25-110 kDa) antibody

Melanoma Mouse IgG1 NKI/C3 Hu P
1 ml / €560.00
  Abnova Taiwan Corp.

Melanoma antibody

Melanoma Mouse HMB45 + DT101 + BC199 + T311 Hu C, ICC/IF, P
0.25 ml / €470.00
  Novus Biologicals Inc.

9 Item(s)

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