Melanophilin antibody

Principal name

Melanophilin antibody

Alternative names for Melanophilin antibody

Mlph, Ln, Slac2a, Exophilin-3, Leaden protein, Synaptotagmin-like protein 2a, Slp homolog lacking C2 domains a

SwissProt ID

Q91V27 (Mouse), Q9BV36 (Human)

Gene ID

171531 (Mlph), 79083 (MLPH)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Melanophilin antibody

Kit Component:
- KN310147G1, Mlph gRNA vector 1 in pCas-Guide vector
- KN310147G2, Mlph gRNA vector 2 in pCas-Guide vector
- KN310147D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Figueiredo AC, Wasmeier C, Tarafder AK, Ramalho JS, Baron RA, Seabra MC. Rab3GEP Is the Non-redundant Guanine Nucleotide Exchange Factor for Rab27a in Melanocytes.
J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID: 18559336
2. Hume AN, Ushakov DS, Tarafder AK, Ferenczi MA, Seabra MC. Rab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes. J Cell Sci. 2007 Sep 1;120(Pt 17):3111-22. PMID: 17698919
3. Hume AN, Tarafder AK, Ramalho JS, Sviderskaya EV, Seabra MC. A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes. Mol Biol Cell. 2006 Nov;17(11):4720-35. PMID: 16914517

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Melanophilin antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MLPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808503)(1:150) Mouse IgG2a OTI6E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Melanophilin (C-term) antibody

TA305809 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice.  The upper band may be the stacking-spearating gel interface. Data kindly provided by A N Hume, Imperial College. Goat Aff - Purified Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Melanophilin antibody

WB Suggested Anti-MLPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Melanophilin (C-term) antibody

Immunohistochemistry analysis in human stomach tissue (Formalin-fixed, Paraffin-embedded) using Melanophilin / MLPH  antibody Cat.-No. AP52706PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MLPH antibdy  for IHC; Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Melanophilin antibody

Melanophilin Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Melanophilin antibody

Wild-type C57bl6 derived melanocytes were stained with the Mlph polyclonal antibody ( Cat # PAB6173 ) ( 0.5ug/ml ) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College. Goat Ms E, ICC/IF, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Melanophilin (C-term) antibody

Melanophilin Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Melanophilin

Melanophilin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Melanophilin

Melanophilin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MLPH 293T Cell Transient Overexpression Lysate(Denatured)

MLPH 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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