Melanophilin antibody

Principal name

Melanophilin antibody

Alternative names for Melanophilin antibody

Mlph, Ln, Slac2a, Exophilin-3, Leaden protein, Synaptotagmin-like protein 2a, Slp homolog lacking C2 domains a

SwissProt ID

Q91V27 (Mouse), Q9BV36 (Human)

Gene ID

171531 (Mlph), 79083 (MLPH)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Melanophilin antibody

Kit Component:
- KN310147G1, Mlph gRNA vector 1 in pCas-Guide vector
- KN310147G2, Mlph gRNA vector 2 in pCas-Guide vector
- KN310147D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Figueiredo AC, Wasmeier C, Tarafder AK, Ramalho JS, Baron RA, Seabra MC. Rab3GEP Is the Non-redundant Guanine Nucleotide Exchange Factor for Rab27a in Melanocytes.
J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID: 18559336
2. Hume AN, Ushakov DS, Tarafder AK, Ferenczi MA, Seabra MC. Rab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes. J Cell Sci. 2007 Sep 1;120(Pt 17):3111-22. PMID: 17698919
3. Hume AN, Tarafder AK, Ramalho JS, Sviderskaya EV, Seabra MC. A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes. Mol Biol Cell. 2006 Nov;17(11):4720-35. PMID: 16914517

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Melanophilin antibody

  MLPH mouse monoclonal antibody, clone OTI6E8 (formerly 6E8)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MLPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808503)(1:150) Mouse IgG2a OTI6E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Melanophilin (C-term) antibody

  Goat Anti-Melanophilin (mouse) Antibody  
TA305809 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice.  The upper band may be the stacking-spearating gel interface. Data kindly provided by A N Hume, Imperial College. Goat Aff - Purified Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Melanophilin antibody

  Rabbit Polyclonal Anti-MLPH Antibody  
WB Suggested Anti-MLPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Melanophilin (C-term) antibody

Immunohistochemistry analysis in human stomach tissue (Formalin-fixed, Paraffin-embedded) using Melanophilin / MLPH  antibody Cat.-No. AP52706PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MLPH antibdy  for IHC; Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

MLPH - human gene knockout kit via CRISPR

MLPH - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MLPH - human gene knockout kit via CRISPR

MLPH - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MLPH - human gene knockout kit via CRISPR

MLPH - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MLPH - human gene knockout kit via CRISPR

MLPH - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mlph - mouse gene knockout kit via CRISPR

Mlph - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mlph - mouse gene knockout kit via CRISPR

Mlph - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mlph - mouse gene knockout kit via CRISPR

Mlph - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mlph - mouse gene knockout kit via CRISPR

Mlph - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MLPH (mGFP-tagged) - Human melanophilin (MLPH), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, MLPH (mGFP-tagged) - Human melanophilin (MLPH), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, MLPH (Myc-DDK tagged) - Human melanophilin (MLPH), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, MLPH (Myc-DDK tagged) - Human melanophilin (MLPH), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mlph (GFP-tagged) - Mouse melanophilin (Mlph), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mlph (GFP-tagged) - Mouse melanophilin (Mlph), 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.

Lenti ORF particles, Mlph (Myc-DDK-tagged) - Mouse melanophilin (Mlph), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mlph (Myc-DDK-tagged) - Mouse melanophilin (Mlph), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.

16 Item(s)

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