Melanophilin antibody

Principal name

Melanophilin antibody

Alternative names for Melanophilin antibody

Mlph, Ln, Slac2a, Exophilin-3, Leaden protein, Synaptotagmin-like protein 2a, Slp homolog lacking C2 domains a

SwissProt ID

Q91V27 (Mouse), Q9BV36 (Human)

Gene ID

79083 (MLPH), 171531 (Mlph)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of Melanophilin antibody

This gene encodes a member of the exophilin subfamily of Rab effector proteins. The protein forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. A similar protein complex in mouse functions to tether pigment-producing organelles called melanosomes to the actin cytoskeleton in melanocytes, and is required for visible pigmentation in the hair and skin. A mutation in this gene results in Griscelli syndrome type 3, which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Figueiredo AC, Wasmeier C, Tarafder AK, Ramalho JS, Baron RA, Seabra MC. Rab3GEP Is the Non-redundant Guanine Nucleotide Exchange Factor for Rab27a in Melanocytes.
J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID: 18559336
2. Hume AN, Ushakov DS, Tarafder AK, Ferenczi MA, Seabra MC. Rab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes. J Cell Sci. 2007 Sep 1;120(Pt 17):3111-22. PMID: 17698919
3. Hume AN, Tarafder AK, Ramalho JS, Sviderskaya EV, Seabra MC. A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes. Mol Biol Cell. 2006 Nov;17(11):4720-35. PMID: 16914517

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Melanophilin antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MLPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808503)(1:150) Mouse IgG2a OTI6E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Melanophilin (C-term) antibody

TA305809 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice.  The upper band may be the stacking-spearating gel interface. Data kindly provided by A N Hume, Imperial College. Goat Aff - Purified Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Melanophilin antibody

WB Suggested Anti-MLPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Melanophilin (C-term) antibody

Immunohistochemistry analysis in human stomach tissue (Formalin-fixed, Paraffin-embedded) using Melanophilin / MLPH  antibody Cat.-No. AP52706PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MLPH antibdy  for IHC; Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Melanophilin antibody

Melanophilin Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Melanophilin antibody

Wild-type C57bl6 derived melanocytes were stained with the Mlph polyclonal antibody ( Cat # PAB6173 ) ( 0.5ug/ml ) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College. Goat Ms E, ICC/IF, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Melanophilin (C-term) antibody

Melanophilin Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Melanophilin antibody

NB 300-943 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may be the stacking-spearating gel interface. Data kindly provided by A N Hume, Imperial College. Goat IgG Aff - Purified Ms E, ICC/IF, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Melanophilin antibody

Immunohistochemistry-Paraffin: Melanophilin Antibody [NBP1-84765] Immunohistochemical staining of human prostate shows strong positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Melanophilin

Melanophilin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Melanophilin

Melanophilin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MLPH 293T Cell Transient Overexpression Lysate(Denatured)

MLPH 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

12 Item(s)

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