MELK antibody

Principal name

MELK antibody

Alternative names for MELK antibody

Maternal embryonic leucine zipper kinase, KIAA0175, hMELK

SwissProt ID

Q14680 (Human), Q61846 (Mouse)

Gene ID

9833 (MELK), 17279 (Melk)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of MELK antibody

Melk is a Serine/threonine-protein kise involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Melk Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It Plays a key role in cell proliferation. It is required for proliferation of embryonic and posttal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. It's also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus and may also play a role in primitive hematopoiesis.

General readings

1. Lin ML et al. Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family. Breast Cancer Res. 2007;9(1):R17.
PMID: 17280616

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MELK antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining. Mouse IgG2a 2G2 Purified Hu E, ICC/IF, P
0.1 mg / €430.00
  Acris Antibodies GmbH

MELK (C-term) antibody

  Goat Polyclonal Antibody against MELK  
TA302675 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane).  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MELK antibody

  Rabbit Polyclonal Anti-MELK Antibody  
WB Suggested Anti-MELK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MELK antibody

  Rabbit Polyclonal Anti-Melk Antibody  
Host: Rabbit; Target Name: Melk; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MELK antibody

The anti-MRLK Pab is used in Western blot to detect MRLK in mouse brain tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MELK (C-term) antibody

The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MELK antibody

Immunohistochemistry (IHC) analyzes of MELK antibody (Cat.-No.: AP20443PU-N) in paraffin-embedded human liver carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MELK antibody

MELK Goat Purified Hu WB
0.1 mg / €550.00
  Acris Antibodies GmbH

MELK antibody

Western blot analysis of extracts from K562 cells, using MELK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

MELK antibody

Western blot analysis of extracts from K562 cells, using MELK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

MELK - human gene knockout kit via CRISPR

MELK - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MELK - human gene knockout kit via CRISPR

MELK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MELK - human gene knockout kit via CRISPR

MELK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MELK - human gene knockout kit via CRISPR

MELK - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Melk - mouse gene knockout kit via CRISPR

Melk - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Melk - mouse gene knockout kit via CRISPR

Melk - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Melk - mouse gene knockout kit via CRISPR

Melk - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Melk - mouse gene knockout kit via CRISPR

Melk - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MELK overexpression lysate

MELK overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

MELK-knockout Cell lysate

MELK-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

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