MELK antibody

Principal name

MELK antibody

Alternative names for MELK antibody

Maternal embryonic leucine zipper kinase, KIAA0175, hMELK

SwissProt ID

Q14680 (Human), Q61846 (Mouse)

Gene ID

9833 (MELK), 898332 (Q9X0N8), 17279 (Melk)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of MELK antibody

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

General readings

1. Lin ML et al. Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family. Breast Cancer Res. 2007;9(1):R17.
PMID: 17280616

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MELK antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining. Mouse IgG2a 2G2 Purified Hu E, ICC/IF, P
0.1 mg / €430.00
  Acris Antibodies GmbH

MELK (C-term) antibody

TA302675 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane).  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
20 µg / €200.00
  Acris Antibodies GmbH

MELK (C-term) antibody

TA302675 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane).  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MELK antibody

WB Suggested Anti-MELK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MELK antibody

Host: Rabbit; Target Name: Melk; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MELK antibody

The anti-MRLK Pab is used in Western blot to detect MRLK in mouse brain tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MELK (C-term) antibody

The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MELK antibody

Immunohistochemistry (IHC) analyzes of MELK antibody (Cat.-No.: AP20443PU-N) in paraffin-embedded human liver carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MELK antibody

MELK Goat Purified Hu WB
0.1 mg / €550.00
  Acris Antibodies GmbH

MELK antibody

MELK Mouse IgG1 4D8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

MELK antibody

The MELK polyclonal antibody ( Cat # PAB2705 ) is used in Western blot to detect MRLK in mouse brain tissue lysate. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MELK antibody

MELK Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MELK antibody

MELK polyclonal antibody ( Cat # PAB6013 ) staining ( 0.1 µg/ml ) of human testes lysate ( RIPA buffer, 1.4E5 cells per lane ) . Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MELK antibody

Western blot analysis of extracts from K562 cells, using MELK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

MELK antibody

Western blot analysis of extracts from K562 cells, using MELK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

MELK antibody

Human HT1080; WB Suggested Anti-MELK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; MELK antibody - middle region (ARP46207_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MELK antibody

Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MELK antibody

Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MELK antibody

Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MELK antibody

Western Blot: MELK Antibody [NB100-782] - NB100-782 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.  Online Catalogue  NAC1 to Quaking > Goat Anti-PPP1R15A Antibody Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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