MER2 antibody

Principal name

MER2 antibody

Alternative names for MER2 antibody

Meiotic recombination 2 protein, REC107, YJR021C, J1462

SwissProt ID

P21651 (Yeast)

Gene ID

853478 (REC107)

Available reactivities

Ye (Yeast), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB)

Background of MER2 antibody

Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that Sphase CDK associates with the replication fork.

4 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MER2 pSer30 antibody

Western blot using affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first  immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphor-ylated protein. The primary antibody was used at a 1:5,000 dilution. Rabbit IgG Aff - Purified S. cerevisiae E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

MER2 (26-35) antibody

Western blot using affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution. Rabbit IgG Aff - Purified S. cerevisiae E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

MER2 pSer30 antibody

Western blot using MER2 (phospho S30) polyclonal antibody ( Cat # PAB11308 ) shows detection of phosphorylated MER2 in whole cell extracts.
Cells were either wild type ( lane 1 ) or contained MER2 deletions ( lane 2 ).
Extracts were prepared from cells 4 hr after initiation of meiosis.
Proteins were obtained using TCA precipitation.
The primary antibody was used at a 1 : 7,500 dilution.
Secondary antibody was used at 1:5,000 dilution.
Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD. Rabbit Ye E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MER2 antibody

Western blot using MER2 polyclonal antibody ( Cat # PAB11356 ) shows detection of phosphorylated and unphosphorylated MER2 in wild type, phosphatase treated and mutant cells.
Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated MER2.
Lane 2 contains the same preparation after treatment with phosphatase.
Lane 3 contains MER2-S30A protein as a phosphorylation control.
This antibody is reactive with both phosphor-ylated and unphosphorylated MER2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.
Personal Communication. MichaelLichten, NIH, CCR, Bethesda, MD. Rabbit Ye E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

4 Item(s)

per page
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