Alternative names for Mesothelioma antibody
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Paraffin Sections (P)
Background of Mesothelioma antibody
This monoclonal antibody was generated by the immunization of mice with the mesothelioma cell line SPC111. It has been reported that this antibody reacts with a 200 kDa surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells, but does not react with most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas. In the two studies cited below, this antibody was reactive with all of the mesotheliomas examined, with 50%-99% of cells of both pleural and peritoneal mesotheliomas stained. Literature reports have indicated that the antibody does not bind with many common breast (MCF-7, T47D, ZR75-1, BT20, SJC38), ovarian (OVCA429, OVCA432, OVCA434), colon (SW480, SW620, CX1, WIDR, Co112), and renal-cell (FOHN, ZNH1) carcinoma cell lines, nor with several leukemia (K562, U937, CEM) or fibroblast (WI38, R2F) cell lines. Some reactivity has been reported with some pulmonary tumors of squamous-cell and small-cell carcinoma origin, adenocarcinoma of the breast, and ovary, and gastrointestinal adenocarcinomas. Also, melanomas and poorly differentiated pulmonary adenocarcinoma tumors have reacted with this antibody. The user is strongly encouraged to review the cited literature concerning the reported reactivity of this antibody.