MGMT antibody

Principal name

MGMT antibody

Alternative names for MGMT antibody

Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase

SwissProt ID

P16455 (Human), P24528 (Rat), P26187 (Mouse), P26188 (Yeast), P29177 (Bovin)

Gene ID

25332 (Mgmt), 4255

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Hst (Hamster), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of MGMT antibody

MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53. Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.

General readings

Svrcek, M., et al. Gut 59(11):1516-1526 (2010)
Palli, D., et al. Mutagenesis 25(6):569-575 (2010)
Kim, M., et al. Cancer Sci. 101(11):2436-2442 (2010)
Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010)
Jacob, R., et al. Anticancer Res. 30(7):2561-2566 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB52 Purified Hu, Mky P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

MGMT antibody

MGMT Mouse IgG1 UMAB56 Purified Hu, Mky P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (UM800006; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB52 Purified Hu, Mky P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

MGMT antibody

MGMT Mouse IgG1 UMAB56 Purified Hu, Mky P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800732) Mouse IgG1 OTIB12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800856) Mouse IgG1 OTI4E6 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150) Mouse IgG1 OTI6C4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150) Mouse IgG1 OTI5A7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150) Mouse IgG1 OTI2E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150) Mouse IgG1 OTI4F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150) Mouse IgG1 OTI2F10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150) Mouse IgG1 OTI10H9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MGMT antibody

Western Blot: MGMT Antibody (MT 23.2) - Western blot analysis of MGMT (MT 23.2) antibody  - Detection of MGMT in MCF7 lysate using anti-MGMT at 1:1000 dilution. Mouse IgG2b MT 23.2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MGMT (N-term) antibody

MGMT Antibody (N-term) (Cat. #TA324503) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MGMT antibody

MGMT Antibody (C-term) (Cat. #TA324927) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow). Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MGMT antibody

MGMT Antibody(Cat. #TA325178) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow). Mouse IgG1 888CT22.2.4 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MGMT antibody

MGMT Antibody (Cat. #TA328166) western blot analysis in MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow). Mouse IgG1 888CT22.2.4 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MGMT antibody

Western blot analysis of MGMT expression in Jurkat cells. Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MGMT antibody

ELISA: MGMT Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

MGMT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT. Mouse IgG1 OTID5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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