MHC Class I H-2 Db antibody

Principal name

MHC Class I H-2 Db antibody

Alternative names for MHC Class I H-2 Db antibody

H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain

SwissProt ID

P01899 (Mouse)

Gene ID

14964 (H2-D1), 225825 (Cd226)

Available reactivities

Ms (Mouse)

Available hosts

Mouse, Rat

Available applications

Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Cytotoxicity Assay (CT)

Background of MHC Class I H-2 Db antibody

In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen).
MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant beta 2 Microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.

General readings

1. Hammerling,G.J., Hammerling, U. and Lemke, H. (1979b) Isolation of twelve monoclonal antibodies against Ia
and H-2 antigens. Serological characterization and reactivity with B and T lymphocytes. Immunogenetics, 8,433 (1979).
2. Klein, J., Huang, H.S., Lemke, H., Hammerling, G. J. and Hammerling, U. (1979). Serological analysis of H-2 and Ia molecules with monoclonal antibodies. Immunogenetics, 8, 419 (1979).
3. Klein, J., Gotze, D., Hammerling , G. J. and Lemke, H. Nomenclature for H-2 and Ia antigens defined by monoclonal antibodies. Immunogenetics, 9, 503-507 (1979).
4. Klein, J., Figueroa, F., and Chella, S. David. H-2 Haplotypes, Genes and Antigens: Second Listing. II. The H-2
Complex. Immunogenetics, 17, 553-596 (1983).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 28-14-8 Purified Ms C, CT, F, FN, IP
0.5 mg / €310.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 28-14-8 Biotin Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

Immunofluorescent Staining: Splenocytes from DBA1/J mice were stained with Mouse anti-Mouse H-2Db-FITC. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG2a 28-14-8 FITC Ms F
0.5 mg / €370.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 28-14-8 Low Endotoxin Ms F
0.5 mg / €420.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 28-14-8 PE Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 27-11-13S Biotin Ms F
0.5 mg / €340.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 27-11-13S FITC Ms F
0.5 mg / €360.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 27-11-13S Low Endotoxin Ms CT, F
0.5 mg / €430.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 27-11-13S Purified Ms CT, F
0.5 mg / €320.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 27-11-13S PE Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Rat IgG2a B22-249.R1 FITC Ms CT
0.1 mg / €260.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Rat IgG2a B22-249.R1 Purified Ms CT
0.25 mg / €320.00
  Acris Antibodies GmbH

MHC Class I H-2 Db antibody

Splenocytes from DBA1/J mice were stained with H2-D2 monoclonal antibody, clone 28-14-8 ( Cat # MAB3258 )-FITC. Small lymphocytes were then gated and analyzed on a FACScanTM flow cytometer ( BDIS, San Jose, CA ). Mouse IgG2a 28-14-8 Ms C, F
0.5 mg / €390.00
  Abnova Taiwan Corp.

MHC Class I H-2 Db antibody

MHC Class I H-2 Db Mouse IgG2a 27-11-13S Ms C, F
0.5 mg / €390.00
  Abnova Taiwan Corp.

MHC Class I H-2 Db antibody

C57BL/6 splenocytes stained with KH95 PE Mouse IgG2b KH95 Biotin Ms F
0.5 mg / €780.00
  Novus Biologicals Inc.

MHC Class I H-2 Db antibody

C57BL/6 splenocytes stained with KH95 PE Mouse IgG2b KH95 FITC Ms F
0.5 mg / €780.00
  Novus Biologicals Inc.

MHC Class I H-2 Db antibody

C57BL/6 mouse splenocytes stained with 28-14-8 PE Mouse IgG2a 28-14-8 Aff - Purified Ms C, F, IP, P
0.5 mg / €400.00
  Novus Biologicals Inc.

MHC Class I H-2 Db antibody

Flow cytometry / FACS analysis: beta 2 Microglobulin, H-2Db antigen Antibody (28-14-8) [NBP1-28181] - Splenocytes from DBA1/J mice were stained with mouse anti-mouse H-2Db-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). Mouse IgG2a 28-14-8 Purified Ms C, F, P
0.5 mg / €420.00
  Novus Biologicals Inc.

MHC Class I H-2 Db antibody

Flow cytometry / FACS analysis: beta 2 Microglobulin, H-2Db antigen Antibody (28-14-8) [FITC] [NBP1-28182] - Splenocytes from DBA1/J mice were stained with mouse anti-mouse H-2Db-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). Mouse IgG2a 28-14-8 FITC Ms C, F, P
0.25 mg / €600.00
  Novus Biologicals Inc.

MHC Class I H-2 Db antibody

Flow cytometry / FACS analysis: beta 2 Microglobulin, H-2Db antigen Antibody (28-14-8) [Biotin] [NBP1-28183] - Splenocytes from DBA1/J mice were stained with mouse anti-mouse H-2Db-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). Mouse IgG2a 28-14-8 Biotin Ms C, F, P
0.5 mg / €780.00
  Novus Biologicals Inc.

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