MIA antibody

Principal name

MIA antibody

Alternative names for MIA antibody

Melanoma inhibitory activity, Melanoma-derived growth regulatory protein

SwissProt ID

Q16674 (Human), Q28038 (Bovin), Q61865 (Mouse), Q62946 (Rat)

Gene ID

8190 (MIA), 12587 (Mia1), 81510 (Mia)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of MIA antibody

Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.

General readings

Blesch A, Bosserhoff AK, et al. (1994) Cancer Res 54 (21): 5695–701.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIA antibody

Staining of Human Glioma lysate using MIA antibody Cat.-No AP32142PU-N at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MIA antibody

  Rabbit polyclonal anti-MIA antibody  
Immunohistochemistry analysis of paraffin-embedded human heart tissue using MIA antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MIA antibody

  Anti-Human MIA Rabbit Polyclonal Antibody  
IHC of human pancreas infiltrating ductal adenocarcinoma tissue.  The recommended concentration is 1.0 ug/ml - 2.0 ug/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen.  Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIA antibody

MIA Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

MIA antibody

MIA Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

MIA antibody

MIA Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIA antibody

MIA Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

MIA antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MIA Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

MIA antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MIA Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Mia - mouse gene knockout kit via CRISPR

Mia - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mia - mouse gene knockout kit via CRISPR

Mia - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mia - mouse gene knockout kit via CRISPR

Mia - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mia - mouse gene knockout kit via CRISPR

Mia - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MIA

  Recombinant protein of human melanoma inhibitory activity (MIA)  
MIA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MIA

  Recombinant protein of human melanoma inhibitory activity (MIA)  
MIA Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

MIA (transcript variant 1)

  Purified recombinant protein of Human melanoma inhibitory activity (MIA), transcript variant 1.  
MIA Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 20 µg / €199.00

Special Price: 20 µg / €139.00

  OriGene Technologies, Inc.

MIA (25-131, His-tag)

MIA Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

MIA (25-131, His-tag)

MIA Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MIA (mGFP-tagged) - Human melanoma inhibitory activity (MIA), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, MIA (mGFP-tagged) - Human melanoma inhibitory activity (MIA), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, MIA (Myc-DDK tagged) - Human melanoma inhibitory activity (MIA), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, MIA (Myc-DDK tagged) - Human melanoma inhibitory activity (MIA), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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