MICA antibody

Principal name

MICA antibody

Alternative names for MICA antibody

MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

SwissProt ID

Q29983 (Human)

Gene ID

4276, 100507436 (MICA)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Functional assay (FN), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), ELISA (capture) (E(capture)), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MICA antibody

MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes. The standard product used in this kit is recombinant MICA, which is composed of 286 amino acids with the molecular mass of 59KDa.

General readings

Strausberg, R.L, et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99 (26), 16899-16903

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MICA + MICB antibody

Immunoprecipitation of MICA/B from HeLa cells with mouse IgG2a (1) or AM26693AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with anti MICA/B (BAMO1). Mouse IgG2a BAMO3 Azide Free Hu E, E(detection), F, IP
0.1 mg / €320.00
  Acris Antibodies GmbH

MICA + MICB antibody

Western blot analysis of MICA/B expression in 293T (1), Jurkat (2), HeLa (3) and DLD-1 (4) using AM26694AF-N. Mouse IgG1 BAMO1 Azide Free Hu F, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

MICA antibody

Western blot analysis of MICA Antibody (Center) (Cat. #TA325054) in MDA-MB231 cell line lysates (35ug/lane). MICA (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MICA antibody

Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MICA antibody

Host: Rabbit; Target Name: MICA; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MICA antibody

WB Suggested Anti-MICA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MICA antibody

WB Suggested Anti-MICA Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MICA (+ MICB) antibody

MICA Mouse IgG2a 6D4 Biotin Hu C, F
0.1 mg / €430.00
  Acris Antibodies GmbH

MICA (+ MICB) antibody

MICA Mouse IgG2a 6D4 FITC Hu F
0.1 mg / €410.00
  Acris Antibodies GmbH

MICA (+ MICB) antibody

MICA Mouse IgG2a 6D4 FITC Hu
25 µg / €190.00
  Acris Antibodies GmbH

MICA (+ MICB) antibody

MICA Mouse IgG2a 6D4 Low Endotoxin Hu C, F, FN, IP
0.5 mg / €570.00
  Acris Antibodies GmbH

MICA (+ MICB) antibody

MICA Mouse IgG2a 6D4 Purified Hu C, F, IP
0.2 mg / €490.00
  Acris Antibodies GmbH

MICA (+ MICB) antibody

MICA Mouse IgG2a 6D4 Purified Hu C, IP
25 µg / €180.00
  Acris Antibodies GmbH

MICA antibody

Flow cytometric analysis of MICA expression on 293T cells (left) and Jurkat cells (right). Open histograms indicates the reaction of isotypic control to the cells. Shaded histograms indicates the reaction of AM26571AF-N to the cells. Mouse IgG1 AMO1 Azide Free Hu E, E(capture), F
0.1 mg / €320.00
  Acris Antibodies GmbH

MICA antibody

MICA Mouse IgG1 B-N31 Azide Free Hu
0.2 mg / €270.00
  Acris Antibodies GmbH

MICA antibody

A typical staining pattern with the B-Z26 monoclonal antibody on U266 cell line Mouse IgG2a B-Z26 Purified Hu E, F
2ml (200T) / €250.00
  Acris Antibodies GmbH

MICA antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) anti MICA antibody (Cat#AP07325PU-N) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MICA Control Peptide

MICA
50 µg / €160.00
  Acris Antibodies GmbH

MICA antibody

Western Blot analysis of MICA expression in transfected 293T cell line (H00004276-T01) by MICA MaxPab polyclonal antibody.

Lane 1: MICA transfected lysate(42.13 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MICA antibody

Western Blot analysis of MICA expression in transfected 293T cell line (H00004276-T02) by MICA MaxPab polyclonal antibody.

Lane 1: MICA transfected lysate(42.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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