MID2 / RNF60 antibody

Principal name

MID2 / RNF60 antibody

Alternative names for MID2 / RNF60 antibody

Midin-2, Midline defect 2, Midline-2, RING finger protein 60, FXY2, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Tripartite motif-containing protein 1

SwissProt ID

Q9QUS6 (Mouse), Q9UJV3 (Human)

Gene ID

11043 (MID2), 23947 (Mid2)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MID2 / RNF60 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

General readings

Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B.
MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407. PMID: 10400986

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MID2 / RNF60 (C-term) antibody

TA302853 (1µg/ml) staining of mouse heart lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MID2 / RNF60 antibody

WB Suggested Anti-Mid2 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Small Intestine Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MID2 / RNF60 antibody

Host: Rabbit; Target Name: MID2; Sample Tissue: NCI-H226 Whole Cell lysates; Antibody Dilution: 1.0 ug/mlMID2 is supported by BioGPS gene expression data to be expressed in NCIH226 Rabbit IgG Purified Can, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MID2 / RNF60 (723-735) antibody

Kidney: Fromalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MID2 / RNF60 antibody

Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T01) by MID2 MaxPab polyclonal antibody.

Lane 1: MID2 transfected lysate(75.35 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T02) by MID2 MaxPab polyclonal antibody.

Lane 1: MID2 transfected lysate(75.35 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T02) by MID2 MaxPab polyclonal antibody.

Lane 1: MID2 transfected lysate(75.35 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

MID2 polyclonal antibody ( Cat # PAB6327 ) staining ( 1 µg/ml ) of mouse heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

NB100-1167 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining Goat IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MID2 / RNF60 antibody

Immunohistochemistry-Paraffin: MID2 Antibody [NBP1-81862] - Immunohistochemical staining of human pancreas shows strong granular positivity in exocrine pancreas. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MID2 / RNF60 (transcript variant 1)

MID2 / RNF60 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MID2 / RNF60

MID2 / RNF60 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MID2 / RNF60

MID2 / RNF60 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

MID2 / RNF60

MID2 / RNF60 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MID2 / RNF60

MID2 / RNF60 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MID2 293T Cell Transient Overexpression Lysate(Denatured)

MID2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

MID2 293T Cell Transient Overexpression Lysate(Denatured)

MID2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

MID2 overexpression lysate

MID2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MID2 overexpression lysate

MID2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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