MID2 / RNF60 antibody

Principal name

MID2 / RNF60 antibody

Alternative names for MID2 / RNF60 antibody

Midin-2, Midline defect 2, Midline-2, RING finger protein 60, FXY2, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Tripartite motif-containing protein 1

SwissProt ID

Q9QUS6 (Mouse), Q9UJV3 (Human)

Gene ID

11043 (MID2), 23947 (Mid2)

Available reactivities

Hu (Human), Ms (Mouse), Bt (Bat), Can (Canine), Chk (Chicken), Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Rt (Rat), Tr (Turkey), Bear, Bov (Bovine), GP (Guinea Pig)

Available hosts

Mouse, Goat, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of MID2 / RNF60 antibody

Kit Component:
- KN310090G1, Mid2 gRNA vector 1 in pCas-Guide vector
- KN310090G2, Mid2 gRNA vector 2 in pCas-Guide vector
- KN310090D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B.
MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407. PMID: 10400986

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MID2 / RNF60 (C-term) antibody

  Goat Polyclonal Antibody against MID2  
TA302853 (1µg/ml) staining of mouse heart lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MID2 / RNF60 antibody

  Rabbit Polyclonal Anti-Mid2 Antibody  
WB Suggested Anti-Mid2 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Small Intestine Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MID2 / RNF60 antibody

  Rabbit Polyclonal Anti-MID2 Antibody - C-terminal region  
Host: Rabbit; Target Name: MID2; Sample Tissue: NCI-H226 Whole Cell lysates; Antibody Dilution: 1.0 ug/mlMID2 is supported by BioGPS gene expression data to be expressed in NCIH226 Rabbit IgG Purified Can, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MID2 / RNF60 (723-735) antibody

Kidney: Fromalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MID2 / RNF60 antibody

Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T01) by MID2 MaxPab polyclonal antibody.

Lane 1: MID2 transfected lysate(75.35 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T02) by MID2 MaxPab polyclonal antibody.

Lane 1: MID2 transfected lysate(75.35 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T02) by MID2 MaxPab polyclonal antibody.

Lane 1: MID2 transfected lysate(75.35 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MID2 / RNF60 antibody

MID2 polyclonal antibody ( Cat # PAB6327 ) staining ( 1 µg/ml ) of mouse heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

MID2 - human gene knockout kit via CRISPR

MID2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MID2 - human gene knockout kit via CRISPR

MID2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MID2 - human gene knockout kit via CRISPR

MID2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MID2 - human gene knockout kit via CRISPR

MID2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mid2 - mouse gene knockout kit via CRISPR

Mid2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mid2 - mouse gene knockout kit via CRISPR

Mid2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mid2 - mouse gene knockout kit via CRISPR

Mid2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mid2 - mouse gene knockout kit via CRISPR

Mid2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MID2 / RNF60 (transcript variant 1)

  Recombinant protein of human midline 2 (MID2), transcript variant 1  
MID2 / RNF60 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MID2 / RNF60

MID2 / RNF60 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MID2 / RNF60

MID2 / RNF60 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

MID2 / RNF60

MID2 / RNF60 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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