Midkine antibody

Principal name

Midkine antibody

Alternative names for Midkine antibody

MK, MDK, MK1, NEGF2, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, Neurite outgrowth-promoting factor 2

SwissProt ID

P12025 (Mouse), P21741 (Human), P24052 (Chick), Q9R1S9 (Rat)

Gene ID

4192 (MDK), 6419263 (IPR003180), 17242 (Mdk)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of Midkine antibody

Midkine (MK, also called neurite growth promoting factor 2, NEGF-2), a product of a retinoic acid responsive gene, is a secreted 13 kDa protein belonging to the family of heparin binding growth/differentiation factors. MK shares 45% sequence identity with other member of this family called Pleiotrophin (HB-GAM). Midkine is composed of two domains held together by disulfide linkages. The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity. Part of the MK activity is enhanced by dimerization of MK. Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation. In the adult MK expression is restricted. In addition to normal development, MK is also involved in the pathogenesis of diseases e.g. inflammatory diseases, human carcinomas such as esophageal, stomach, colon, pancreatic, thyroid, lung, urinary, hepatocellular, neuroblastoma, glioblastoma, Wilm´s tumor etc. High MK levels are associated with poor prognosis in some type of cancer. The increased expresion in many carcinomas indicates that MK can be applied to the diagnosis of malignancy. Midkine is expressed during the reparative stage of bone fractures, also supresses infection of certain viruses including HIV in target cells. Anti-apoptotic and cell protecting activity of midkine makes it to be a promissing in therapy.

General readings

Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010)
Kerzerho, J., et al. J. Immunol. 185(1):418-423(2010)
Yao, X., et al. Acta Pharmacol. Sin. 31(5):629-637(2010)
Zhang, Z.H., et al. Cell Prolif. 43(2):184-194(2010)
Rice, G.E., et al. J. Exp. Clin. Cancer Res. 29, 62 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Midkine (C-term) antibody

Confocal immunofluorescent analysis of Midkine Antibody (C-term) Cat.-No AP52643PU-N with HepG2 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Midkine antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MDK antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Midkine antibody

Western blot analysis of extracts of SW480 cells, using MDK antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Midkine antibody

To detect hMidkine by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Midkine as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Midkine antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MDK rabbit polyclonal antibody. (TA590690) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Midkine antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDK (RC221818, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDK.(1:2000) Mouse IgG1 OTI6C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Midkine antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDK (RC221818, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDK.(1:2000) Mouse IgG1 OTI8C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Midkine antibody

Midkine Rabbit Aff - Purified Hu C, E, WB
50 µg / €370.00
  Acris Antibodies GmbH

Midkine antibody

Midkine Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Midkine antibody

Midkine Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Midkine antibody

Midkine Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Midkine antibody

Midkine Rabbit Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Midkine antibody

Western Blot analysis of MDK expression in transfected 293T cell line (H00004192-T01) by MDK MaxPab polyclonal antibody.

Lane 1: MDK transfected lysate(15.84 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Midkine antibody

Midkine Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Midkine antibody

Western Blot analysis of MDK expression in transfected 293T cell line (H00004192-T02) by MDK MaxPab polyclonal antibody.

Lane 1: MDK transfected lysate(15.73 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Midkine antibody

Immunoprecipitation of MDK transfected lysate using anti-MDK MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MDK purified MaxPab mouse polyclonal antibody (B02P) (H00004192-B02P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Midkine antibody

Western Blot analysis of MDK expression in transfected 293T cell line (H00004192-T02) by MDK MaxPab polyclonal antibody.

Lane 1: MDK transfected lysate(15.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Midkine antibody

Midkine Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Midkine antibody

Western blot analysis of Midkine expression in HeLa (A); mouse kidney (B); rat liver (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Midkine antibody

Western blot analysis of Midkine expression in HeLa (A); mouse kidney (B); rat liver (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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