Midline-1 antibody

Principal name

Midline-1 antibody

Alternative names for Midline-1 antibody

MID1, FXY, RNF59, TRIM18, XPRF, Midin, RING finger protein 59, Midline 1 RING finger protein, Tripartite motif-containing protein 18, Putative transcription factor XPRF

SwissProt ID

O15344 (Human), O70583 (Mouse), P82458 (Rat)

Gene ID

4281 (MID1), 17318 (Mid1), 54252

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Midline-1 antibody

MID1 is a member of the tripartite motif (TRIM) family, also known as the \'RING-B box-coiled coil\' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing; however, the full length nature of two variants has not been determined.

General readings

Winter,J., et al., (2004) Hum. Genet. 114 (6), 541-552

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Midline-1 antibody

  Rabbit polyclonal anti-TRI18/MID1 antibody  
Western blot analysis of extracts from 293 cells, using TRI18 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Midline-1 antibody

  Rabbit Polyclonal Anti-MID1 Antibody  
WB Suggested Anti-MID1 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Midline-1 antibody

  Rabbit Polyclonal Anti-MID1 Antibody - N-terminal region  
Host: Rabbit; Target Name: MID1; Sample Tissue: Transfected 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Midline-1 antibody

  Rabbit Polyclonal Anti-TRI18 Antibody  
Western blot analysis on A549 and HuvEc cell lysate using TRI18 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Midline-1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRI18 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

Midline-1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRI18 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

MID1 - human gene knockout kit via CRISPR

MID1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MID1 - human gene knockout kit via CRISPR

MID1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MID1 - human gene knockout kit via CRISPR

MID1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MID1 - human gene knockout kit via CRISPR

MID1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mid1 - mouse gene knockout kit via CRISPR

Mid1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mid1 - mouse gene knockout kit via CRISPR

Mid1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mid1 - mouse gene knockout kit via CRISPR

Mid1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mid1 - mouse gene knockout kit via CRISPR

Mid1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Midline-1 (transcript variant 1)

  Purified recombinant protein of Homo sapiens midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1  
Midline-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Midline-1 (transcript variant 3)

  Recombinant protein of human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3  
Midline-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Midline-1 (transcript variant 4)

  Recombinant protein of human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 4  
Midline-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MID1 (mGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, MID1 (mGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, MID1 (mGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, MID1 (mGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, MID1 (mGFP-tagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 6, 200 uL, >10^7 TU/mL

Lenti ORF particles, MID1 (mGFP-tagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 6, 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

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