MIF antibody

Principal name

MIF antibody

Alternative names for MIF antibody

Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF

SwissProt ID

P14174 (Human), P30904 (Rat), P34884 (Mouse), P80177 (Bovin), P80928 (Pig), Q1ZZU7 (Sheep)

Gene ID

4282 (MIF), 81683 (Mif), 17319

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Can (Canine), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Chicken, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)

Background of MIF antibody

Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/ml to 10 ng/ml of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18-kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.

General readings

Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29; [Epub ahead of print]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIF (C-term) antibody

TA303028 (0.03µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

Western blot analysis of MIF expression in lysates from Jurkat (Lane 1, 2), 3T3 (Lane 3) and rat kidney (Lane 4). Rabbit Purified Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

WB analysis of Immunochemical's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. Chicken Purified Hu E, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

Western blot analysis of extracts of Jurkat cell lines, using MIF antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

WB Suggested Anti-MIF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIF (103-115) antibody

Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) using MIF Antibody Goat IgG Aff - Purified Bov, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Por, Prim, Rb, Rt, Sh, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MIF (C-term) antibody

Immunohistochemical analysis of paraffin embedded rat kidney sections, staining MIF in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIF antibody

SM6013 MIF antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast. Mouse IgG1 4E4 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

MIF antibody

SM6013 MIF antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast. Mouse IgG1 4E4 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

MIF antibody

MIF MaxPab rabbit polyclonal antibody. Western Blot analysis of MIF expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MIF antibody

Immunoperoxidase of monoclonal antibody to MIF on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma tissue. [antibody concentration sup. ug/ml] Mouse IgG1 2A10-4D3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MIF antibody

Western blot analysis of HL - 60 extract was resolved by SDS - PAGE , transferred to PVDF membrane and probed with MIF monoclonal antibody , clone 4E4 ( 1 : 1000 ) ( Cat # MAB1071 ) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 4E4 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

MIF antibody

MIF polyclonal antibody ( Cat # PAB6796 ) ( 0.03 µg/ml ) staining of human thymus lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MIF antibody

Immunohistochemical analysis of paraffin-embedded rat kidney sections, stain MIF in cytoplasm DAB chromogenic reaction. Rabbit Hu, Rt P
0.1 mg / €390.00
  Abnova Taiwan Corp.

MIF antibody

Western blot analysis of MIF expression in lysates from Jurkat ( Lane 1, 2 ) , NIH/3T3 ( Lane 3 ) and rat kidney ( Lane 4 ) . Rabbit Bov, Hu, Ms, Rt IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MIF antibody

MIF Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MIF antibody

MIF Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

MIF antibody

MIF Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €330.00
  Boster Immunoleader

MIF antibody

Western Blot: MIF Antibody [NB100-1789]-(0.01µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MIF antibody

MIF Mouse IgG1 4E4 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

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