MIF antibody

Principal name

MIF antibody

Alternative names for MIF antibody

Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF

SwissProt ID

P14174 (Human), P30904 (Rat), P34884 (Mouse), P80177 (Bovin), P80928 (Pig), Q1ZZU7 (Sheep)

Gene ID

4282 (MIF), 103694877 (LOC103694877), 17319 (Mif), 81683

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Tr (Turkey), Can (Canine)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of MIF antibody

Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/ml to 10 ng/ml of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18-kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.

General readings

Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIF antibody

SM6013 MIF antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast. Mouse IgG1 4E4 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

MIF antibody

SM6013 MIF antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast. Mouse IgG1 4E4 Purified Hu E, F, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

MIF (C-term) antibody

  Goat Polyclonal Antibody against MIF  
TA303028 (0.03µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

  Rabbit polyclonal anti-macrophage migration inhibitory factor (MIF) antibody  
Western blot analysis of MIF expression in lysates from Jurkat (Lane 1, 2), 3T3 (Lane 3) and rat kidney (Lane 4). Rabbit Purified Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

  Chicken polyclonal anti-MIF antibody  
WB analysis of Immunochemical's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. Chicken Purified Hu E, WB
0.5 mg / €415.00
  OriGene Technologies, Inc.

MIF antibody

  Rabbit anti-MIF Polyclonal Antibody  
Western blot analysis of extracts of Jurkat cell lines, using MIF antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIF antibody

  Rabbit Polyclonal Anti-MIF Antibody  
WB Suggested Anti-MIF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIF (103-115) antibody

Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) using MIF Antibody Goat IgG Aff - Purified Bov, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Por, Prim, Rb, Rt, Sh, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MIF (C-term) antibody

Immunohistochemical analysis of paraffin embedded rat kidney sections, staining MIF in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIF antibody

MIF Rabbit Purified Ms, Rt IP, WB
0.2 mg / €290.00
  Torrey Pines Biolabs

MIF antibody

MIF Rabbit Azide Free Ms, Rt IP, WB
0.2 mg / €290.00
  Torrey Pines Biolabs

CRISPR

Catalog No. Species Donor Vector  

Mif - mouse gene knockout kit via CRISPR

Mif - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mif - mouse gene knockout kit via CRISPR

Mif - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mif - mouse gene knockout kit via CRISPR

Mif - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mif - mouse gene knockout kit via CRISPR

Mif - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MIF

  Recombinant protein of human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF)  
MIF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MIF

  Purified recombinant protein of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF).  
MIF Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.

Regular Price: 25 µg / €199.00

Special Price: 25 µg / €139.00

  OriGene Technologies, Inc.

MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.5 mg / €990.00
  Acris Antibodies GmbH

MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.1 mg / €340.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Mouse Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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