MINPP1 antibody

Principal name

MINPP1 antibody

Alternative names for MINPP1 antibody

Multiple inositol polyphosphate phosphatase 1, MIPP, UNQ900/PRO1917, Inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase, Ins(1,3,4,5)P(4) 3-phosphatase

SwissProt ID

O35217 (Rat), Q9UNW1 (Human), Q9Z2L6 (Mouse)

Gene ID

9562 (MINPP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of MINPP1 antibody

Multiple inositol polyphosphatase is only enzyme known to hydrolyze the abundant metabolites of phosphorylated inositol signaling molecule inositol penta- and hexa-phosphates. Sequence analysis of plant and fruit fly MINPP homolog¡¦s supports the hypothesis that the MINPP enzymes constitute a distinct evolutionary group within the histidine phosphatase family. Minpp chicken homolog is a histdine phosphatase HiPER1 that has a role on growth plate chondrocyte differentiation. The human and murine homolog of Minpp1 mRNA expression was found in variety of tissues compared to much restricted expression of chick homolog HiPER1. The murine and human Minpp is 80% homologous to rat protein and 56% to HiPER (1). During osteogenesis in growth plate chondrocytes the levels of Minpp initially increase 2-3fold and then reduced to the basal levels with a concomitant decrease in Ins5 but no change in Ins6 levels in ATDC5 cells (2). The Minpp1 is largely a endoplasmic reticulum associated protein, however, a truncated for Minpp1 has been shown in the cytoplasm of cells that might be involved in conversion of Ins5 and Ins6 in to a Ca second messenger 1, 4, 5 inositol triphosphatse in independent inositol lipid breakdown. Minpp1 is compartmentalized in the endoplasmic reticulum (ER) lumen. The Minpp-deficient mice are viable, fertile and without obvious defect but have 30-40% higher Ins5 and Ins6 levels that are reduced to normal by reintroducing Minpp. Reintroduction of Minpp led to generation of a truncated Minpp-cytosolic form, lacking its Er targeting N-terminus that dephosphorylates the polyinositol phosphtates (3).
The human Minpp1 is localized on human chromosome10q23, the assignment of Minpp1 on chromosome 10 paces it proximal to tumor suppressor protein Pten on chromosome 10 that is most frequently mutated in cancer (4). The comparison of human Minpp1 and chick HiPER1 revealed significant homology in the core protein but the ER targeting domain was divergent. The catalytic domain has a Histidine, replacing with Alanine led to complete loss of activity (5). Minpp1 is a 487amino acid protein, approximately 58kDa, is expressed in multiple splice variant forms.

General readings

Newman, A.B., et al. J. Gerontol. A Biol. Sci. Med. Sci. 65(5):478-487(2010)
Cho, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(16):5998-6003(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MINPP1 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma reacted with bMINPP1 Antibody (N-term) Cat.-No AP52700PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MINPP1 antibody

Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308980 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MINPP1 antibody

Western blot analysis of extracts from 293 cells, using MINPP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MINPP1 antibody

WB Suggested Anti-MINPP1 Antibody; Titration: 1.0 ug/ml; Positive Control: MDA-MB-435S Whole CellMINPP1 is supported by BioGPS gene expression data to be expressed in MDA-MB435 Rabbit IgG Purified Bov, Can, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MINPP1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MINPP1 mouse monoclonal antibody (TA507234) at 1:150 dilution. Mouse IgG1 OTI1B2 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MINPP1 Control Peptide

MINPP1
0.25 mg / €250.00
  Acris Antibodies GmbH

MINPP1 (26-46) antibody

Western blot of AP10148PU-N (1:500). Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

MINPP1 (C-term) antibody

Western blot analysis of MINPP1 Antibody (C-term) Cat.-No AP52699PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the MINPP1 antibody detected the MINPP1 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MINPP1 antibody

MINPP1 Rabbit Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

MINPP1 antibody

Western blot analysis of extracts from 293 cells, using MINPP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

MINPP1 antibody

Western blot analysis of extracts from 293 cells, using MINPP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

MINPP1 antibody

Western Blot: MINPP1 Antibody [NBP1-32872] - (30 ug of whole cell lysate) A: Molt-4  7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

MINPP1 antibody

Immunohistochemistry: MIPP Antibody [NBP1-33491] - Immunohistochemical analysis of MIPP on paraffin-embedded DLD-1 xenograft, using NBP1-33491. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

MINPP1 antibody

Immunohistochemistry-Paraffin: MINPP1 Antibody [NBP1-82644] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MINPP1

MINPP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MINPP1 (transcript variant 2)

MINPP1 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MINPP1 (full length, C-term DDK tag, transcript variant 1)

MINPP1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

MINPP1 (transcript variant 1)

MINPP1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

MINPP1

MINPP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MINPP1

MINPP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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