MIP1 alpha / CCL3 antibody

Principal name

MIP1 alpha / CCL3 antibody

Alternative names for MIP1 alpha / CCL3 antibody

CCL-3, MIP-1-alpha, C-C motif chemokine 3, MIP1A, Small-inducible cytokine A3, SCYA3, LD78 alpha, G0S19-1 protein, Tonsillar lymphocyte LD78 alpha protein, Macrophage Inflammatory Protein 1 alpha, G0/G1 switch regulatory protein 19-1

SwissProt ID

P10147 (Human), P10855 (Mouse), P50229 (Rat), Q8SQA6 (Bovin)

Gene ID

6348 (CCL3), 20302 (Ccl3), 25542

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit, Sheep, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Neutralisation (NEUT), ELISA (detection) (E(detection))

Background of MIP1 alpha / CCL3 antibody

Human Macrophage inflammatory protein-1alpha (MIP-1 alpha; MIP-1a) belongs to the chemokine family. Chemokines are small secreted molecules containing 4 conserved cysteine residues and 2 disulfide linkages. The first two cysteine residues of chemokine molecules may be in one of the following configurations, CC or CXC and this defines the two major chemokine sub-families. MIP-1 is a CC chemokine and its recent designation is chemokine ligand 3 ( CCL3 ). MIP-1 alpha is constitutively expressed from platelets and bone marrow CD34 progenitor cells. MIP-1 alpha expression can also be induced by bacteria or bacterial products such as LPS in monocyte/macrophages, dendritic cells, neutrophils, microglial cells and in activated B and T cells. Expression of MIP-1 alpha has also been described in non-immune somatic cells such as fibroblasts, mucosal epithelial cells, osteoblasts and vascular smooth muscle cells. As a chemokine, MIP-1 alpha plays a role in the recruitment of leukocytes to the sites of infection, and is involved in the activation of cells and the inflammatory response. MIP-1 alpha is chemotactic for monocytes/macrophages, T cells, neutrophils, eosinophils, basophils, dendritic cells and NK cells. MIP-1 alpha also plays a role in the transendothelial migration of monocytes, T lymphocytes, neutrophils, and dendritic cells at sites of inflammation. MIP-1 alpha and MIP-1 beta are the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1 alpha has been shown to inhibit different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) in a dose-dependent manner. MIP-1 alpha is a peptide of 92 amino acids. MIP-1 alpha occurs naturally as a homodimer or a heterodimer with MIP-1 beta. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

1. Abu-Taha et al. M. Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.
The Journal of Immunology.;183(2):1393-402. 7/15/2009.
2. Mateo, T. et al. Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
The Journal of Immunology.;176(9):5577-86 5/1/2006.
3. Czermak, B.J. et al. Synergistic Enhancement of Chemokine Generation and Lung Injury by C5a or the Membrane Attack Complex of Complement. American Journal of Pathology.;154(5):1513-24. 1/1/1999.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIP1 alpha / CCL3 (77-92) antibody

Human lung stained with anti-MIP-1 alpha antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 (77-92) antibody

Human lung stained with anti-MIP-1 alpha antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 (77-92) antibody

Human lung stained with anti-MIP-1 alpha antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

Western blot analysis of MIP-1-alpha with RAW264. Rabbit Purified Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

Anti-mouse MIP-1a in WB shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated (0.1 µg, 7.8 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was incubated with Rockland's, Inc. Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. Rabbit IgG Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Human MIP-1alpha; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-1alpha;. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Human MIP-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-1alpha; (TA328387) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1alpha;. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Human MIP-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-1alpha;  as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1alpha;. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Murine MIP-1alpha; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1alpha;. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Murine MIP-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MIP-1alpha; (TA328538) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine MIP-1alpha;. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Rat MIP-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat MIP-1alpha; (TA328591) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP-1alpha;. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

To detect Rat MIP-1alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MIP-1alpha; (TA328590) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1alpha;. Rabbit IgG Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 alpha / CCL3 antibody

MIP1 alpha / CCL3 Rabbit IgG Serum Hu E
1 ml / €1,150.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 (C-term) antibody

Western blot analysis of CCL3 Antibody (C-term) (AP17929PU-N) in mouse liver tissue lysates (35ug/lane). CCL3 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

Immunohistochemistry analyzes of MIP-1α antibody (Cat.-No.: AP20656PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P
0.1 mg / €350.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

MIP1 alpha / CCL3 Mouse IgG1 125 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

MIP1 alpha / CCL3 Mouse IgG2a 116 Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

MIP1 alpha / CCL3 Mouse IgG2a 199 Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

MIP1 alpha / CCL3 Mouse IgG2a 548 Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

MIP1 alpha / CCL3 antibody

MIP1 alpha / CCL3 Mouse IgG1 400 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

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