MIP1 beta / CCL4 antibody

Principal name

MIP1 beta / CCL4 antibody

Alternative names for MIP1 beta / CCL4 antibody

CCL-4, MIP-1-beta, LAG1, MIP1B, SCYA4, Small-inducible cytokine A4, PAT744, Protein H400, SIS-gamma, HC21, T-cell activation protein 2, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1-beta, G-26 T-lymphocyte-secreted protein

SwissProt ID

P13236 (Human), P14097 (Mouse), P50230 (Rat), Q17QA1 (Bovin), Q711P4 (Pig), Q90826 (Chick)

Gene ID

388372 (CCL4L2), 20303 (Ccl4), 116637, 6351 (CCL4), 396668

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse, Chicken, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), Frozen Sections (C), Neutralisation (NEUT), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of MIP1 beta / CCL4 antibody

Macrophage inflammatory protein 1 beta (MIP-1 beta; MIP-1b) belongs to the chemokine family. Chemokines are small secreted molecules containing 4 conserved cysteine residues and 2 disulfide linkages. The first two cysteine residues of chemokine molecules may be in one of the following configurations, CC or CXC and this defines the two major chemokine sub-families. MIP-1 beta is a CC chemokine and its recent designation is chemokine ligand 4, (CCL4). MIP-1 beta is expressed from activated monocytes, T-lymphocytes, B-lymphocytes, NK cells, dendritic cells, neutrophils, and its expression can be inhibited by IL-10 and IL-4. It is also expressed from non-immune somatic cells such as brain endothelial cells, vascular smooth muscle cells, the pineal gland, prostate cells, the liver, spleen and fetal microglial cells. MIP-1 beta exhibits chemotactic properties for monocytes, T-lymphocytes, neutrophils, eosinophils, immature dendritic cells and NK cells, and plays a role in the transendothelial migration and activation of monocytes, T-lymphocytes, neutrophils and dendritic cells. MIP-1 beta is synthesized as a precursor protein of 92 amino acids that includes a cleaved 23 amino acid signal sequence. It has a molecular mass of approximately 8 kDa and may exist as a symmetrical homodimer or as a heterodimer with the related chemokine, MIP-1 alpha. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

General / background references:Leith JG et al. CD8+ T-cell-mediated suppression of HIV-1 long terminal repeat-driven gene expression is not modulated by the CC chemokines RANTES, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta. AIDS 11:575-80 (1997). PubMed PMID: 9108938
Graham GJ et al. Identification and characterization of an inhibitor of haemopoietic stem cell proliferation. Nature 344:442-4 (1990). PubMed PMID: 2320111
Lipes MA et al. Identification, cloning, and characterization of an immune activation gene. Proc Natl Acad Sci U S A 85:9704-8 (1988). PubMed PMID: 2462251

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

Immunohistochemistry: This MIP1 beta antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma.  The recommended concentration is 1.0 µg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

Immunohistochemistry: This MIP1 beta antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma.  The recommended concentration is 1.0 µg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

anti-Human MIP-1ß antibody shows detection of a band ~15 kDa in size corresponding to recombinant human MIP-1ß. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

Western blot analysis of CCL4 in rat brain tissue lysate with CCL4 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 (Center) antibody

CCL4 Antibody (Center) (Cat. #TA324311) western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the CCL4 antibody detected CCL4 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

To detect Murine MIP-1beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1beta. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

To detect Murine MIP-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MIP-1beta (TA328540) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine MIP-1beta. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCL4 (RC210481, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCL4. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Aff - Purified Ms E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Aff - Purified Ms E, WB
50 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

CCL4 polyclonal antibody (A01), Lot # 051121JC01 Western Blot analysis of CCL4 expression in IMR-32 ( Cat # L008V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MIP1 beta / CCL4 antibody

Western Blot analysis of CCL4 expression in transfected 293T cell line (H00006351-T01) by CCL4 MaxPab polyclonal antibody.

Lane 1: CCL4 transfected lysate(10.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Ms, Rt IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit IgG Aff - Purified Ms WB
0.1 mg / €290.00
  Boster Immunoleader

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Goat Aff - Purified Hu E, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

MIP1 beta / CCL4 antibody

ELISA: Macrophage Inflammatory Protein 1 beta Antibody [33030002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

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