MIP1 beta / CCL4 antibody

Principal name

MIP1 beta / CCL4 antibody

Alternative names for MIP1 beta / CCL4 antibody

CCL-4, MIP-1-beta, LAG1, MIP1B, SCYA4, Small-inducible cytokine A4, PAT744, Protein H400, SIS-gamma, HC21, T-cell activation protein 2, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1-beta, G-26 T-lymphocyte-secreted protein

SwissProt ID

P13236 (Human), P14097 (Mouse), P50230 (Rat), Q17QA1 (Bovin), Q711P4 (Pig), Q90826 (Chick)

Gene ID

388372 (CCL4L2), 20303 (Ccl4), 116637, 6351 (CCL4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of MIP1 beta / CCL4 antibody

MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

Immunohistochemistry: This MIP1 beta antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma.  The recommended concentration is 1.0 µg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

Immunohistochemistry: This MIP1 beta antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma.  The recommended concentration is 1.0 µg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

anti-Human MIP-1ß antibody shows detection of a band ~15 kDa in size corresponding to recombinant human MIP-1ß. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

Western blot analysis of CCL4 in rat brain tissue lysate with CCL4 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 (Center) antibody

CCL4 Antibody (Center) (Cat. #TA324311) western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the CCL4 antibody detected CCL4 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

To detect Murine MIP-1beta by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1beta. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

To detect Murine MIP-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MIP-1beta (TA328540) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine MIP-1beta. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCL4 (RC210481, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCL4. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Aff - Purified Ms E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Aff - Purified Ms E, WB
50 µg / €230.00
  Acris Antibodies GmbH

MIP1 beta / CCL4 antibody

CCL4 polyclonal antibody (A01), Lot # 051121JC01 Western Blot analysis of CCL4 expression in IMR-32 ( Cat # L008V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MIP1 beta / CCL4 antibody

Western Blot analysis of CCL4 expression in transfected 293T cell line (H00006351-T01) by CCL4 MaxPab polyclonal antibody.

Lane 1: CCL4 transfected lysate(10.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Ms, Rt IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit IgG Aff - Purified Ms WB
0.1 mg / €290.00
  Boster Immunoleader

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Goat Aff - Purified Hu E, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

MIP1 beta / CCL4 antibody

MIP1 beta / CCL4 Rabbit Purified Ms, Rt IP, WB
0.5 mg / €290.00
  Torrey Pines Biolabs

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