MIP3 alpha / CCL20 antibody

Principal name

MIP3 alpha / CCL20 antibody

Alternative names for MIP3 alpha / CCL20 antibody

CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker, Macrophage inflammatory protein 3 alpha, Liver and activation-regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1

SwissProt ID

O89093 (Mouse), P78556 (Human), P97884 (Rat), Q8SQB1 (Bovin)

Gene ID

6364 (CCL20), 29538 (Ccl20), 20297

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Macaque, Mky (Monkey), Prim (Primate), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), ELISA (capture) (E(capture))

Background of MIP3 alpha / CCL20 antibody

Macrophage inflammatory protein (MIP)-3alpha/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3alpha plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3alpha is the most potent chemokine inducing the selective migration of in vitrogenerated CD34(+) hematopoietic progenitor cell-derived LC precursors and skin LCs in accordance with the restricted MIP-3alpha receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3alpha is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked with an antibody against MIP-3alpha. (c) In vivo, MIP-3alpha is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP- 3alpha upregulation appear associated with an increase in LC turnover. (d) The secretion of MIP-3alpha is strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1beta plus tumor necrosis factor alpha) or T cell signals.(Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18;Homey B. et al, 2000,J.Immunol.164:6621-32).

General readings

Schutyser E, et al. (2000) J. Immunol. 165:4470–7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIP3 alpha / CCL20 antibody

  Anti-CCL20 Mouse Monoclonal Antibody  
Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG1 Purified Hu E, P
0.5 mg / €400.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Rabbit polyclonal MIP-3 alpha antibody  
WB using anti-Human MIP-3a antibody shows detection of a band ~26 kDa in size corresponding to recom-binant human MIP-3a (lane 1). The identity of the lower molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000. Rabbit IgG Ig Fraction Hu
0.1 mg / €356.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Rabbit polyclonal MIP 3 alpha antibody  
WB using anti-Mouse MIP3a antibody shows detection of a band ~11 kDa in size corresponding to recombinant mouse MIP3a. Primary antibody was used at 1:1,000 dilution. Secondary antibody HRP Goat anti-Rabbit IgG antibody was used at 1:40,000 dilution. Rabbit IgG Purified Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Mouse monoclonal MIP-3a antibody  
Western Blot of Human MIP 3a (Mouse) Antibody Lane 1: human recombinant MIP-3a Lane 2: mouse recombinant MIP-3a Primary antibody: Human MIP 3a (RAT) Antibody at 0.5 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rabbit at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C Mouse 3N2D9 Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Mouse monoclonal Anti-Human MIP-3a (MOUSE) Biotin Conjugated antibody  
Western Blot of Human MIP 3a (Mouse) Antibody Lane 1: human recombinant MIP-3a Lane 2: mouse recombinant MIP-3a Primary antibody: Human MIP 3a (RAT) Antibody at 0.5 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rabbit at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C Mouse 3N2D9 Purified Hu WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Rat monoclonal anti-MIP-3a antibody  
Western Blot of Mouse MIP 3a (RAT) antibody Lane 1: mouse recombinant MIP-3a Lane 2: human recombinant MIP-3a Primary antibody: MIP 3a antibody at 0.1 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rat at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C Rat 4N5F7 Purified Ms WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Rat monoclonal Anti-Mouse MIP-3a (RAT) Biotin Conjugated antibody  
Western Blot of Mouse MIP 3a (RAT) antibody Lane 1: mouse recombinant MIP-3a Lane 2: human recombinant MIP-3a Primary antibody: MIP 3a antibody at 0.1 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rat at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C Rat 4N5F7 Purified Ms WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Biotinylated Anti-Human MIP-3a Rabbit Polyclonal Antibody  
To detect Human MIP-3alpha; by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3alpha;. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

  Anti-Human MIP-3a Rabbit Polyclonal Antibody  
To detect Human MIP-3alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-3alpha; (TA328392) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3alpha;. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 antibody

MIP3 alpha / CCL20 Mouse IgG1 Azide Free Hu E, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 (33-44) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hu, Mky, Prim E, P
50 µg / €470.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 antibody

AP31717PU-N CCL20 antibody staining of paraffin embedded Human Liver at 6 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Hu, Por E, P
0.1 mg / €350.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 antibody

MIP3 alpha / CCL20 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 antibody

MIP3 alpha / CCL20 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 antibody

MIP3 alpha / CCL20 Rabbit Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 antibody

MIP3 alpha / CCL20 Rabbit Aff - Purified Hu E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MIP3 alpha / CCL20 antibody

IHC staining of human tonsil frozen section with clone 206D9 (DX0420) Mouse IgG1 206D9.05 Alexa 488 Hu E(detection), P
50 µg / €390.00
  Dendritics SAS

MIP3 alpha / CCL20 antibody

IHC staining of human tonsil frozen section with clone 206D9 (DX0420) Mouse IgG1 206D9.05 Alexa Fluor 488 Hu
0.1 mg / €520.00
  Dendritics SAS

MIP3 alpha / CCL20 antibody

IHC staining of human tonsil frozen section with clone 206D9 (DX0420) Mouse IgG1 206D9.05 Alexa 546 Hu E(detection), P
50 µg / €390.00
  Dendritics SAS

MIP3 alpha / CCL20 antibody

IHC staining of human tonsil frozen section with clone 206D9 (DX0420) Mouse IgG1 206D9.05 Alexa Fluor 546 Hu
0.1 mg / €520.00
  Dendritics SAS

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