MITF antibody

Principal name

MITF antibody

Alternative names for MITF antibody

Microphthalmia-associated transcription factor, Mi-protein

SwissProt ID

O75030 (Human), O88368 (Rat), Q08874 (Mouse)

Gene ID

4286 (MITF)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Electrophoretic Mobility Shift Assay (EMSA), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of MITF antibody

MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retil pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Altertively spliced transcript variants encoding different isoforms have been identified. This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retil pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Altertively spliced transcript variants encoding different isoforms have been identified.

General readings

General / background references:Bertolotto C et al. Microphthalmia gene product as a signal transducer in cAMP-induced differentiation of melanocytes. J Cell Biol 142:827-35 (1998). PubMed PMID: 9700169
Aberdam E et al. Involvement of microphthalmia in the inhibition of melanocyte lineage differentiation and of melanogenesis by agouti signal protein. J Biol Chem 273:19560-5 (1998). PubMed PMID: 9677380
Steingramsson E et al. Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequences. Nat Genet 8:256-63 (1994). PubMed PMID: 7874168
Hodgkinson CA et al. Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper protein. Cell 74:395-404 (1993). PubMed PMID: 8343963

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MITF antibody

Figure 1. Immunohistochemical staining using microphthalmia antibody (AM05308PU-N) on formalin fixed, paraffin embedded human melanoma. Mouse IgG1 C5 Purified Hu, Ms, Rt C, EMSA, IP, P, WB
0.1 mg / €570.00
  Acris Antibodies GmbH

MITF pSer180 antibody

Immunohistochemistry (IHC) analyzes of p-MITF Antibody Cat.-No AP01837PU-N  (pSer180) in paraffin-embedded human breast carcinoma tissue at 1/100. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MITF antibody

Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using MITF (Ab-180/73) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MITF antibody

Predicted band size: 47 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MITF antibody

Predicted band size: 47 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MITF antibody

Western blot analysis of MITF expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MITF antibody

Western blot analysis of extracts of various cell lines, using MITF antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MITF antibody

Immunohistochemistry staining of Paraffin Melanoma tissue by MiTF antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear) Mouse IgG1 C5/D5 Purified Hu P
1 ml / €520.00
  OriGene Technologies, Inc.

MITF antibody

WB Suggested Anti-MITF Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Por, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MITF antibody

WB Suggested Anti-MITF Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysateMITF is supported by BioGPS gene expression data to be expressed in HeLa Rabbit IgG Purified Bov, Gt, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MITF antibody

Western Blot: MiTF Antibody (21D1418) [TA336406] - Analysis of A375 lysate using MITF antibody at 4 ug/ml. Mouse IgG1 21D1418 Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MITF antibody

Formalin-Fixed, Paraffin-Embedded Human melanoma stained with MITF antibody Cat.-No. AM10113SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells. Mouse IgG1 C5+D5 Supernatant Hu P
1 ml / €370.00
  Acris Antibodies GmbH

MITF antibody

Western blot analysis of MITF antibody (Cat.-No.: AP06536PU-N) in extracts from COS7 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MITF (N-term) antibody

Confocal immunofluorescent analysis of  MITF Antibody (N-term) Cat.-No AP52701PU-N with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Aff - Purified Hu F, ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MITF antibody

Western Blot analysis of MITF expression in transfected 293T cell line (H00004286-T01) by MITF MaxPab polyclonal antibody.

Lane 1: MITF transfected lysate(57.2 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MITF antibody

MITF Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

MITF antibody

Western Blot analysis of MITF expression in transfected 293T cell line (H00004286-T02) by MITF MaxPab polyclonal antibody.

Lane 1: MITF transfected lysate(58.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MITF antibody

Western blot analysis of MITF in A-375 lysate. Using MITF monoclonal antibody, clone 21D1418 ( Cat # MAB0103 ) at 4 µg/ml. Mouse IgG1 21D1418 Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

MITF antibody

Immunohistochemical staining usin MITF monoclonal antibody, clone C5 ( Cat # MAB1955 ) on formalin fixed, paraffin embedded human melanoma. Hu, Ms, Rt
0.1 mg / €640.00
  Abnova Taiwan Corp.

MITF pSer180/73 antibody

Western blot analysis of extracts from COS7 cells, using MITF (Phospho-Ser180/73) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

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