MIZF antibody

Principal name

MIZF antibody

Alternative names for MIZF antibody

Histone H4 transcription factor, HINFP, ZNF743, Histone nuclear factor P, MBD2-interacting zinc finger protein, Methyl-CpG-binding protein 2-interacting zinc finger protein

SwissProt ID

Q2TBP2 (Bovin), Q8K1K9 (Mouse), Q9BQA5 (Human)

Gene ID

25988 (HINFP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of MIZF antibody

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. Recently, using MBD2 as bait in two-hybrid screening, a novel zing finger protein, called MIZF has been identified (3). This MBD2-binding zinc finger (MIZF) protein represses transcription by associating with MBD2 and a histone deacetylase complex. It is ubiquitously expressed in all human tissues.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Mitra, P., et al. J. Cell. Physiol. 219(2):438-448(2009)
Medina, R., et al. Biochemistry 47(44):11415-11423(2008)
Medina, R., et al. Cancer Res. 67(21):10334-10342(2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MIZF (Center) antibody

HINFP Antibody (Center) (Cat. #TA324496) western blot analysis in 293 cell line,human placenta,mouse brain and spleen tissue lysates (35ug/lane).This demonstrates the HINFP antibody detected the HINFP protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MIZF antibody

WB Suggested Anti-MIZF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIZF antibody

WB Suggested Anti-MIZF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MIZF antibody

MIZF Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MIZF antibody

Western Blot analysis of MIZF expression in transfected 293T cell line (H00025988-T01) by MIZF MaxPab polyclonal antibody.

Lane 1: MIZF transfected lysate(56.87 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MIZF antibody

Western Blot analysis of MIZF expression in transfected 293T cell line (H00025988-T01) by MIZF MaxPab polyclonal antibody.

Lane 1: MIZF transfected lysate(56.87 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MIZF antibody

Transfected 293T; WB Suggested Anti-MIZF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Transfected 293T; MIZF antibody - middle region (ARP32543_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

MIZF antibody

Transfected 293T; WB Suggested Anti-MIZF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; MIZF antibody - middle region (ARP39196_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MIZF antibody

Western Blot: MIZF Antibody [NB100-56628] - Western blot analysis of MIZF on MOLT-4 cell lysate. Ten microgram of protein was loaded per lane of a mini gel. The antibody was used at 1:500 dilution. Rabbit IgG Serum Hu WB
0.2 ml / €320.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MIZF (transcript variant 1)

MIZF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MIZF

MIZF Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

MIZF

MIZF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MIZF

MIZF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MIZF 293T Cell Transient Overexpression Lysate(Denatured)

MIZF 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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