MLCK antibody

Principal name

MLCK antibody

Alternative names for MLCK antibody

MYLK3, Putative myosin light chain kinase 3

SwissProt ID

Q32MK0 (Human), Q96DV1

Gene ID

91807 (MYLK3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of MLCK antibody

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

General readings

Al-Sadi, R. et al., J. Immunol. 180(8): 5653-5661 (2008)
Chan, J.Y. et al., Circ. Res. 102(5): 571-580 (2008)
Seguchi, O. et al., J. Clin. Invest. 117(10): 2812-2824 (2007)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MLCK antibody

Host: Rabbit; Target Name: MYLK3; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml. Rabbit IgG Purified Bov, Can, Eq, GP, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MLCK (N-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.  (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MLCK antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MLCK (Center) antibody

Western blot analysis in K562 cell line lysates (35ug/lane) using MLCK antibody Cat.-No. AP52792PU-N. This demonstrates this antibody detected the MLCK protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MLCK antibody

Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP1-86648] Immunohistochemical staining of human gall bladder shows moderate cytoplasmic and nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MLCK

MLCK Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MLCK

MLCK Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MYLK3 overexpression lysate

MYLK3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

7 Item(s)

per page
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