MLF1IP antibody

Principal name

MLF1IP antibody

Alternative names for MLF1IP antibody

MLF1-interacting protein, Centromere protein U, CENPU, ICEN24, KLIP1, PBIP, CENP-U(50), Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, Polo-box-interacting protein 1

SwissProt ID

Q2KIW5 (Bovin), Q2Z1W2 (Chick), Q4V8G7 (Rat), Q71F23 (Human), Q8C4M7 (Mouse)

Gene ID

79682 (MLF1IP), 71876 (Mlf1ip), 306464

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Chk (Chicken), Eq (Equine), Por (Porcine)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MLF1IP antibody

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-
dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

General readings

Foltz DR. et al. (2006) Nat Cell Biol. 8:458-469.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MLF1IP (N-term) antibody

  Rabbit polyclonal anti-MLF1 antibody  
Western blot using affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Chimp, Eq, Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MLF1IP antibody

  Rabbit polyclonal Mlf1 phospho T78 antibody (Phospho-specific)  
Western blot using affinity purified anti-MLF1IP pT78 antibody shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006). Rabbit IgG Purified Bov, Can, Chimp, Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MLF1IP antibody

  Rabbit polyclonal anti-MLF1 antibody  
Western blot using affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MLF1IP (C-term) antibody

  Rabbit polyclonal MLF1IP / PBIP1 antibody  
Western blot using affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Chimp, Eq, Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MLF1IP antibody

  Rabbit Polyclonal Anti-CENPU Antibody  
MLF1IP Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MLF1IP antibody

  Rabbit Polyclonal Anti-CENPU Antibody  
MLF1IP Rabbit IgG Purified Bov, Eq, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

MLF1IP pThr78 antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Can, Hu E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MLF1IP pThr78 antibody

Figure 1. Staining MLF1 Interacting Protein (MLF1IP) in Colon adenocarcinoma by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Bov, Can, Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CENPU - human gene knockout kit via CRISPR

CENPU - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CENPU - human gene knockout kit via CRISPR

CENPU - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CENPU - human gene knockout kit via CRISPR

CENPU - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CENPU - human gene knockout kit via CRISPR

CENPU - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cenpu - mouse gene knockout kit via CRISPR

Cenpu - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cenpu - mouse gene knockout kit via CRISPR

Cenpu - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MLF1IP (147-418, His-tag)

MLF1IP Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

MLF1IP (147-418, His-tag)

MLF1IP Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MLF1IP (mGFP-tagged)-Human MLF1 interacting protein (MLF1IP), 200 uL, >10^7 TU/mL

Lenti ORF particles, MLF1IP (mGFP-tagged)-Human MLF1 interacting protein (MLF1IP), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, MLF1IP (Myc-DDK-tagged)-Human MLF1 interacting protein (MLF1IP), 200 uL, >10^7 TU/mL

Lenti ORF particles, MLF1IP (Myc-DDK-tagged)-Human MLF1 interacting protein (MLF1IP), 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mlf1ip (GFP-tagged) - Mouse myeloid leukemia factor 1 interacting protein (Mlf1ip), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mlf1ip (GFP-tagged) - Mouse myeloid leukemia factor 1 interacting protein (Mlf1ip), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Mlf1ip (Myc-DDK-tagged) - Mouse myeloid leukemia factor 1 interacting protein (Mlf1ip), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mlf1ip (Myc-DDK-tagged) - Mouse myeloid leukemia factor 1 interacting protein (Mlf1ip), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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