MLF1IP antibody

Principal name

MLF1IP antibody

Alternative names for MLF1IP antibody

MLF1-interacting protein, Centromere protein U, CENPU, ICEN24, KLIP1, PBIP, CENP-U(50), Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, Polo-box-interacting protein 1

SwissProt ID

Q2KIW5 (Bovin), Q2Z1W2 (Chick), Q4V8G7 (Rat), Q71F23 (Human), Q8C4M7 (Mouse)

Gene ID

79682 (MLF1IP)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Chk (Chicken), Eq (Equine), Ze (Zebrafish), Por (Porcine)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of MLF1IP antibody

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-
dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

General readings

Foltz DR. et al. (2006) Nat Cell Biol. 8:458-469.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MLF1IP (N-term) antibody

Western blot using affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Chimp, Eq, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MLF1IP antibody

Western blot using affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MLF1IP (C-term) antibody

Western blot using affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Chimp, Eq, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MLF1IP antibody

MLF1IP Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MLF1IP antibody

MLF1IP Rabbit IgG Purified Bov, Eq, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

MLF1IP pThr78 antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MLF1IP pThr78 antibody

Figure 1. Staining MLF1 Interacting Protein (MLF1IP) in Colon adenocarcinoma by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MLF1IP pThr78 antibody

Immunohistochemistry affinity purified anti-MLF1IP pT78 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multicancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu E, ICC/IF, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

MLF1IP (C-term) antibody

Western blot using affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Chimp, Eq, Hu E, WB
25 µl / €190.00
  Acris Antibodies GmbH

MLF1IP (N-term) antibody

Western blot using affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Chimp, Eq, Hu E, WB
25 µl / €190.00
  Acris Antibodies GmbH

MLF1IP antibody

Western blot using MLF1IP polyclonal antibody ( Cat # PAB10266 ) shows detection of MLF1IP ( arrowhead ) in HeLa cells transfected with ZZ-tagged MLF1IP ( lane 1 ).
Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for MLF1IP.
Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD. Bov, Chimp, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

MLF1IP antibody

Western blot using MLF1IP polyclonal antibody ( Cat # PAB10267 ) shows detection of endogenous MLF1IP protein ( a tier of four modified protein bands indicated by the arrowheads ) in lysates of Hela cells ( - lane ).
Cells treated with MLF1IP shRNA ( + lane ) show no staining.
The identity of the higher and lower molecular weight bands are unknown.
Primary antibody was used at 1:1,000.
Personal Communication, K.S. Lee, NCI, Bethesda, MD. Bov, Chimp, Chk, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

MLF1IP antibody

Western blot using MLF1IP polyclonal antibody ( Cat # PAB10268 ) shows detection of endogenous MLF1IP protein ( a tier of four modified protein bands indicated by the arrowheads ) in lysates of Hela cells ( - lane ). Cells treated with MLF1IP shRNA ( + lane ) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1 : 1,000.
Personal Communication, K.S. Lee, NCI, Bethesda, MD. Bov, Chimp, Chk, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

MLF1IP antibody

Immunohistochemistry of MLF1IP (phospho T78) polyclonal antibody ( Cat # PAB10333 ) was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Bov, Chimp, Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MLF1IP antibody

Western Blot: MLF1 Interacting Protein Antibody [NB110-85541] - Western blot using NB100-85541 shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells treated with control luciferase shRNA (Lanes 1), and detection of MLF1IP in Hela cells transfected with MLF1IP (Lanes 3). Lane 2: Hela cells treated with MLF1IP shRNA. The identity of the lower molecular weight bands is unknown. Primary antibody was used at 1:1,000. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

MLF1IP pThr78 antibody

MLF1IP Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, ICC/IF, IP, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

MLF1IP antibody

Immunohistochemistry-Paraffin: MLF1 Interacting Protein Antibody [NBP1-77938] - human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.  Tissue was formalin-fixed and paraffin embedded.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Rabbit Aff - Purified Bov, Can, Hu, Mky E, ICC/IF, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

MLF1IP antibody

Western Blot: ECT2 [p Thr790] Antibody [NBP1-77939] affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells.  Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide.  After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Rabbit Aff - Purified Can, Chk, Hu, Mky, Ms, Rt, Ze E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

MLF1IP antibody

Immunohistochemistry-Paraffin: MLF1 Interacting Protein Antibody [NBP1-85689] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MLF1IP (147-418, His-tag)

MLF1IP Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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