MLL / HRX antibody

Principal name

MLL / HRX antibody

Alternative names for MLL / HRX antibody

ALL1, CXXC7,HTRX, KMT2A, TRX1, ALL-1, Zinc finger protein HRX, Histone-lysine N-methyltransferase HRX, Trithorax-like protein, Lysine N-methyltransferase 2A, CXXC-type zinc finger protein 7

SwissProt ID

P55200 (Mouse), Q03164 (Human)

Gene ID

4297 (MLL)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), ChIP assay (ChIP), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Frozen Sections (C)

Background of MLL / HRX antibody

The MLL gene encodes a DNA-binding protein that methylates histone H3 (see MIM 601128) lys4 (H3K4) and positively regulates expression of target genes, including multiple HOX genes (see MIM 142980). MLL is a frequent target for recurrent translocations in acute leukemias that may be characterized as acute myeloid leukemia (AML; MIM 601626), acute lymphoblastic leukemia (ALL), or mixed lineage (biphenotypic) leukemia (MLL). Leukemias with translocations involving MLL possess unique clinical and biologic characteristics and are often associated with poor prognosis. MLL rearrangements are found in more than 70% of infant leukemias, whether the immunophenotype is more consistent with ALL or AML6, but are less frequent in leukemias from older children. MLL translocations are also found in approximately 10% of AMLs in adults, as well as in therapy-related leukemias, most often characterized as AML, that develop in patients previously treated with topoisomerase II inhibitors for other malignancies. More than 50 different MLL fusion partners have been identified. Leukemogenic MLL translocations encode MLL fusion proteins that have lost H3K4 methyltransferase activity. A key feature of MLL fusion proteins is their ability to efficiently transform hematopoietic cells into leukemia stem cells (Krivtsov and Armstrong, 2007 [PubMed 17957188]).[supplied by OMIM]

General readings

1. Armstrong, S. A., Staunton, J. E., Silverman, L. B., Pieters, R., den Boer, M. L., Minden, M. D.,
Sallan, S. E., Lander, E. S., Golub, T. R., Korsmeyer, S. J. MLL translocations specify a distinct
gene expression profile that distinguishes a unique leukemia. Nature Genet. 30: 41-47, 2002.
2. Bruch, J., Wilda, M., Teigler-Schlegel, A., Harbott, J., Borkhardt, A., Metzler, M. Occurrence of
an MLL/LAF4 fusion gene caused by the insertion ins(11;2)(q23;q11.2q11.2) in an infant with acute
lymphoblastic leukemia. (Letter) Genes Chromosomes Cancer 37: 106-109, 2003.
3. Caligiuri, M. A., Strout, M. P., Oberkircher, A. R., Yu, F., de la Chapelle, A., Bloomfield, C. D. The
partial tandem duplication of ALL1 in acute myeloid leukemia with normal cytogenetics or trisomy 11
is restricted to one chromosome. Proc. Nat. Acad. Sci. 94: 3899-3902, 1997.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MLL / HRX (Super Novus Pack) antibody

Detection of the C-terminal 180 kDa Fragment of MLL1 by Western Blot and Immunoprecipitation. Samples: A. Nuclear extract from K562 cells (N; 50 mcg/lane), 293T cells transfected with full-length MLL1 (F; 15 mcg/lane) and 293T cells transfected with the C-terminal 180kDa fragment of MLL1 (C; 15 mcg/lane). B. Nuclear extract (1 mg) from K563 cells. Antibodies: NB 600-257 used at 1 mcg/ml, 0.1 mcg/ml (A) and 0.4 mcg/ml (B) for blotting. Immunoprecipitations were performed using NB 600-256, NB 600-257, and another MLL1 antibody at 1 mcg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 minute (B). Rabbit Aff - Purified Hu, Ms IP, P
4 vials / €930.00
  Novus Biologicals Inc.

MLL / HRX antibody

Anti-MLL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

MLL / HRX antibody

Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

MLL / HRX (3751-3968) antibody

Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and esophagus cancer (right), showing nuclear weak staining with DAB staining using anti-MLL Monoclonal Antibody. Mouse IgG1 10F8D7 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

MLL / HRX (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MLL / HRX antibody

MLL / HRX Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MLL / HRX antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MLL polyclonal antibody ( Cat # PAB2355 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MLL / HRX antibody

MLL / HRX Rabbit IgG Aff - Purified Hu, Ms WB
0.1 mg / €290.00
  Boster Immunoleader

MLL / HRX antibody

ELISA: KMT2A / MLL Antibody [25300002] Rabbit Hu E, P
50 µg / €440.00
  Novus Biologicals Inc.

MLL / HRX antibody

Western Blot: KMT2A/MLL Antibody [NB600-248] - Nuclear extract (NE; 15 and 50 mcg)  and whole  cell lysate (WCL; 15 and 50 mcg) from HeLa cells.  Antibodies:  Affinity purified rabbit anti-MLL1 antibody. Rabbit Aff - Purified Hu, Ms F, IP, P, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

MLL / HRX antibody

Western Blot: MLL-1 Antibody [NB600-249] - Detection of Human and Mouse MLL1 by Western Blot. Samples: Nuclear extract (50 mcg for WT and KO; 10 mcg for EF, EN and EC) from 293T cells that were transfected with MLL1 c-terminal 180 kDa fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kDa fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody: NB600-249 used at the indicated concentrations. Detection: One minute exposure with chemiluminescence. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

MLL / HRX antibody

Detection of the C-terminal 180 kDa Fragment of MLL1 by Western Blot and Immunoprecipitation. Samples: A. Nuclear extract from K562 cells (N; 50 mcg/lane), 293T cells transfected with full-length MLL1 (F; 15 mcg/lane) and 293T cells transfected with the C-terminal 180kDa fragment of MLL1 (C; 15 mcg/lane). B. Nuclear extract (1 mg) from K563 cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody BL1408 (Cat. No. A300-374A) used at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) for blotting. Immunoprecipitations were performed using NB 600-256, NB 600-257, and another MLL1 antibody at 1 mcg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 minute (B). Rabbit Aff - Purified Hu ChIP, IP, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MLL / HRX (C-term) antibody

Detection of the C-terminal 180 kDa Fragment of MLL1 by Western Blot and Immunoprecipitation. Samples: A. Nuclear extract from K562 cells (N; 50 mcg/lane), 293T cells transfected with full-length MLL1 (F; 15 mcg/lane) and 293T cells transfected with the C-terminal 180kDa fragment of MLL1 (C; 15 mcg/lane). B. Nuclear extract (1 mg) from K563 cells. Antibodies: NB 600-257 used at 1 mcg/ml, 0.1 mcg/ml (A) and 0.4 mcg/ml (B) for blotting. Immunoprecipitations were performed using NB 600-256, NB 600-257, and another MLL1 antibody at 1 mcg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 minute (B). Rabbit Aff - Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MLL / HRX antibody

Immunohistochemistry-Paraffin: KMT2A / MLL Antibody (10F8D7) [NBP1-51542] - Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and esophagus cancer (right), showing nuclear weak staining with DAB staining using MLL mouse mAb. Mouse IgG1 10F8D7 Purified Hu E, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

14 Item(s)

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