MLLT2 / AF4 antibody

Principal name

MLLT2 / AF4 antibody

Alternative names for MLLT2 / AF4 antibody

AFF1, PBM1, FEL, AF4/FMR2 family member 1, Proto-oncogene AF4, Protein FEL

SwissProt ID

O88573 (Mouse), P51825 (Human)

Gene ID

4299 (AFF1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C)

Background of MLLT2 / AF4 antibody

Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, which is associated with acute leukemias. The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor in which AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors. MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells. The expression of MLL-AF-4 influences the production of protein cyclin-dependent kinase inhibitor (CDKN1B), suggesting that inhibition of MLL-AF-4 expression may be a powerful and highly specific treatment of chemotherapy-resistant leukemia.

General readings

"The t(4;11) chromosome translocation of human acute leukemias fuses the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene." Gu Y., Nakamura T., Alder H., Prasad R., Canaani O., Cimino G., Croce C.M., Canaani E. Cell 71:701-708(1992)

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MLLT2 / AF4 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MLLT2 / AF4 (N-term) antibody

WB Suggested Anti-Aff1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Mouse Kidney Rabbit Aff - Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MLLT2 / AF4 (1190-1203) antibody

Figure 2. Staining MLLT2 (AFF) in  Brain, Cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

MLLT2 / AF4 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

MLLT2 / AF4 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

MLLT2 / AF4 antibody

Immunohistochemical analysis of AF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MLLT2 / AF4 antibody

Immunohistochemical analysis of AF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MLLT2 / AF4 antibody

Immunohistochemical analysis of AF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MLLT2 / AF4 antibody

AF4 Antibody [NBP1-28705] - Detection of Human AF4 by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Antibodies: Affinity purified rabbit anti-AF4 antibody A302-345A used for IP at 3 mcg/mg lysate.  AF4 was also immunoprecipitated by rabbit anti-AF4 antibody A302-344A, which recognizes an upstream epitope.  For blotting immunoprecipitated AF4, A302-344A was used at 1 mcg/ml, and the ReliaBLOT®  Reagents and Procedures (Cat. No. WB120) were incorporated.  Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

MLLT2 / AF4 antibody

AF4 Antibody [NBP1-28728] - Detection of Human AF4 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.   Antibodies: Affinity purified rabbit anti-AF4 antibody A302-344A used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  AF4 was also immunoprecipitated by rabbit anti-AF4 antibody A302-345A, which recognizes a downstream epitope.  For blotting immunoprecipitated AF4, the ReliaBLOT®  Reagents and Procedures (Cat. No. WB120) were used.  Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Rabbit IgG Aff - Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MLLT2 / AF4 antibody

MLLT2 / AF4 Rabbit IgG Aff - Purified Hu, Ms E, P
0.1 mg / €360.00
  Novus Biologicals Inc.

MLLT2 / AF4 antibody

MLLT2 / AF4 Rabbit IgG Aff - Purified Hu, Ms E, P
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MLLT2 / AF4

MLLT2 / AF4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MLLT2 / AF4

MLLT2 / AF4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

AFF1 overexpression lysate

AFF1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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