MMACHC antibody

Principal name

MMACHC antibody

Alternative names for MMACHC antibody

Methylmalonic aciduria and homocystinuria type C, DKFZp564I122, FLJ25671, RP11-291L19.3, cobalamin deficiency

SwissProt ID

Q5E9C8 (Bovin), Q5ZL21 (Chick), Q9CZD0 (Mouse), Q9Y4U1 (Human)

Gene ID

25974 (MMACHC)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of MMACHC antibody

Kit Component:
- KN223846G1, MMACHC gRNA vector 1 in pCas-Guide vector
- KN223846G2, MMACHC gRNA vector 2 in pCas-Guide vector
- KN223846D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Lerner-Ellis JP.,et al. (2006) Nat Genet. 38(1):93-100.Tsai AC.,et al. (2007) Am. J. Med. Genet. A 143A (20): 2430-4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMACHC antibody

  Rabbit Polyclonal antibody to MMACHC (methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308065 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504954) Mouse IgG1 OTI1A4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI4E9 (formerly 4E9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG1 OTI4E9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG1 OTI2A6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI1B7 (formerly 1B7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG1 OTI1B7 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI1E7 (formerly 1E7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG2a OTI1E7 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI6H5 (formerly 6H5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG1 OTI6H5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI4E12 (formerly 4E12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG1 OTI4E12 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

  MMACHC mouse monoclonal antibody, clone OTI6B5 (formerly 6B5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Mouse IgG2b OTI6B5 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MMACHC antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe. Mouse IgG1 N230/21 Supernatant Hu, Ms ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

MMACHC antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe. Mouse IgG1 N230/21 Purified Hu, Ms ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

MMACHC antibody

Western Blot analysis of MMACHC expression in transfected 293T cell line by MMACHC MaxPab polyclonal antibody.

Lane 1: MMACHC transfected lysate(24.75 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MMACHC antibody

MMACHC MaxPab rabbit polyclonal antibody. Western Blot analysis of MMACHC expression in human liver. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MMACHC (C-term) antibody

Human U937; WB Suggested Anti-MMACHC Antibody. . Titration: 1.0 ug/ml. . Positive Control: U937 Whole Cell; MMACHC antibody - C-terminal region (ARP61734_P050) in Human U937 cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

MMACHC - human gene knockout kit via CRISPR

MMACHC - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MMACHC - human gene knockout kit via CRISPR

MMACHC - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MMACHC - human gene knockout kit via CRISPR

MMACHC - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MMACHC - human gene knockout kit via CRISPR

MMACHC - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MMACHC

  Recombinant protein of human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC)  
MMACHC Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MMACHC (1-282, His-tag)

MMACHC Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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