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MMP-1 antibody

Principal name

MMP-1 antibody

Alternative names for MMP-1 antibody

Interstitial collagenase, Matrix metalloproteinase-1, Fibroblast collagenase, MMP1, CLG

SwissProt ID

P03956 (Human), P13943 (Rabit), P21692 (Pig), P28053 (Bovin), P81563 (Rat), Q12372 (Yeast)

Gene ID

4312 (MMP1)

Ncbi ID

9606, NP_002412, NP_002412.1, NM_001145938, NM_002421

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Rb (Rabbit), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of MMP-1 antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP\'s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP1 is a secreted enzyme which breaks down the interstitial collagens, types I, II, and III.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP\'s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP\'s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

General readings

Beshir, A.B., et al. Cancer Lett. 299(2):137-149(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010)
Malik, N., et al. J. Neurooncol. (2010) In press.
Skorupski, P., et al. Ginekol. Pol. 81(8):594-599(2010)
Mossbock, G., et al. Mol. Vis. 16, 1764-1770 (2010)

Human placenta stained with Anti-MMP-1 antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31184PU-N

Western blot analysis of extracts from HepG2 cells, using MMP-1 Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MMP1 (Cleaved-Phe100) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.

AM06648SU-N

Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

AM06648SU-N

Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.

AM06648SU-N

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

AM06648SU-N

Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).

AM06648SU-N

Staining of FFPE Human prostate cancer (10x and 40x) with MMP-1 Antibody Cat.-No AF9110 (Clone 195-1H6) at 2 µg/ml. Antibody positive in the cytoplasm of epithelial cells and in stroma.

AF9110

Left: Immunohistochemical staining using MMP-1 antibody on colon carcinoma tissue section. Right: Western blot using MMP-1 antibody on recombinant human MMP-1 proenzyme (400 ng/lane).

AM05376PU-N

Western blot (WB) analysis of MMP-1 antibody (Cat.-No.: AP06224PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate treated with LPS
Lane2:Raw264.7 whole cell lysate treated with LPS
Lane3:PC12 whole cell lysate treated with LPS

AP06224PU-N

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)

AP08372PU-N

Human Lung; . Rabbit Anti-MMP1 Antibody. . Catalog Number:. ARP42039 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X

ARP42039_T100

Human Macrophages; species sample : Human. cell/tissue :macrophagesHuman macrophages. dilution :1/200; MMP1 antibody - N-terminal region (ARP42039_T100) in Human Macrophages cells using Immunohistochemistry

ARP42039_T100

Human Intestine; Antibody Titration: 2-10ug/ml. Positive Control: Human Intestine cell lysate; MMP1 antibody - N-terminal region (ARP42039_T100) in Human Intestine cells using Immunohistochemistry

ARP42039_T100

Human; Human Colorectal cancer sample . Human Liver Sample; MMP1 antibody - N-terminal region (ARP42039_T100) in Human cells using Immunohistochemistry

ARP42039_T100

Human Intestine; Rabbit Anti-MMP1 Antibody. Catalog Number: ARP42039. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; MMP1 antibody - N-terminal region (ARP42039_T100) in Human Intestine cells using Immunohistochemistry

ARP42039_T100

Equine cartilage explants; . Sample Type: Equine Cartilage Explants. Primary Dilution: 1:800. Secondary Antibody: Bio-Rad 170-5046. Secondary Dilution::100,000. Image Submitted By: Adam Williams. University of Nottingham.; MMP1 antibody - N-terminal region (ARP42039_T100) in Equine cartilage explants cells using Western Blot

ARP42039_T100

Human Jurkat; WB Suggested Anti-MMP1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; MMP1 antibody - N-terminal region (ARP42039_T100) in Human Jurkat cells using Western Blot

ARP42039_T100

HT29; WB Suggested Anti-MMP1 Antibody . . Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate . Primary Antibody Dilution : 1:500 . Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:2000 . Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore; MMP1 antibody - N-terminal region (ARP42039_T100) in HT29 cells using Western Blot

ARP42039_T100

Human Macrophage; . Sample Type: Human Macrophange Cells. Green: primary. Red: phallodin. Blue: DAPI. Yellow: green/red. Primary. Dilution:. 1:200. Secondary Antibody: anti-Rabbit IgG-FITC. Secondary. Dilution:. 1:1000. Image Submitted By: Milan Fiala. University of California, Los Angeles .; MMP1 antibody - C-terminal region (ARP42040_T100) in Human Macrophage cells using Immunohistochemistry

ARP42040_T100

Human HT1080; WB Suggested Anti-MMP1 Antibody Titration: 2.5ug/ml. Positive Control: HT1080 cell lysate. MMP1 is supported by BioGPS gene expression data to be expressed in HT1080.; MMP1 antibody - C-terminal region (ARP42040_T100) in Human HT1080 cells using Western Blot

ARP42040_T100

MMP1 MaxPab polyclonal antibody. Western Blot analysis of MMP1 expression in human pancreas.

H00004312-B01P

Western Blot analysis of MMP1 expression in transfected 293T cell line (H00004312-T01) by MMP1 MaxPab polyclonal antibody.Lane 1: MMP1 transfected lysate(51.59 KDa).Lane 2: Non-transfected lysate.

H00004312-B01P

Immunoperoxidase of purified MaxPab rabbit antibody to GOT1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

H00004312-D01P

Proximity Ligation Analysis (PLA) of protein-protein interactions between MMP1 and F2R. HeLa cells were stained with anti-MMP1 rabbit purified polyclonal 1:1200 and anti-F2R mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00004312-D01P

MMP1 MaxPab rabbit polyclonal antibody. Western Blot analysis of MMP1 expression in human pancreas.

H00004312-D01P

Western Blot analysis of MMP1 expression in transfected 293T cell line (H00004312-T01) by MMP1 MaxPab polyclonal antibody.Lane 1: MMP1 transfected lysate(54.00 KDa).Lane 2: Non-transfected lysate.

H00004312-D01P

Western blot using MMP1 monoclonal antibody, clone 3B6 ( Cat # MAB1950 ) on recombinant human MMP1 proenzyme (400 ng/lane).

MAB1950

Immunohistochemical staining using MMP1 monoclonal antibody, clone 3B6 ( Cat # MAB1950 ) on colon carcinoma tissue section.

MAB1950

Immunohistochemical staining of MMP1 on formalin fixed, paraffin embedded human placenta with MMP1 polyclonal antibody.

PAB11701

Human placenta stained with MMP1 polyclonal antibody ( Cat # PAB12708 ) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

PAB12708

Western blot analysis of MMP1 22k expression in MCF7 UV-treated (A); mouse liver (B); rat liver (C) whole cell lysates.

Immunohistochemical analysis of MMP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MMP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of MMP1 expression in HeLa (A); MCF7 (B); MDAMB435 (C); Raw264.7 (D); PC12 (E); NIH3T3 (F) whole cell lysates.

Representative Standard Curve

CEK1274

TP302460

TP723319

TP723882

TP760405

TP720322

Western blot analysis of extracts from 293 cells, using MMP1 (Cleaved-Pro269) antibody.The lane on the right is treated with the synthesized peptide.

TA313101

Western blot analysis of extracts of various cell lines, using MMP1 antibody.

TA326985

Western blot analysis on HepG2 cell lysate using MMP1 Antibody

TA347429

Western blot analysis of extracts from HepG2 cells, using MMP-1 antibody (#TA313099).The lane on the right is treated with the synthesized peptide.

TA313099

Western blot analysis of extracts from 549 cells, treated with etoposide (25uM, 24hours), using MMP1 (Cleaved-Phe100) antibody.The lane on the right is treated with the synthesized peptide.

TA313100

Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using MMP1 antibody at dilution 1/55, on the right is treated with the fusion protein. (Original magnification:x 200)

TA323954

Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using MMP1 antibody at dilution 1/55, on the right is treated with the fusion protein. (Original magnification:x200)

TA323954

Predicted band size: 54 kDa . Positive control: PC3 cell and mouse muscle tissue, A375 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: PC3 cell lysate Lane2: Mouse muscle tissue lysate Lane3: A375 cell lysate Lysates: 80 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA323955

Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using MMP1 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x200)

TA323955

MMP1 Antibody (Center) (Cat. #TA324443) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MMP1 antibody detected the MMP1 protein (arrow).

TA324443

MMP1 Antibody (Center) (Cat. #TA324443)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TA324443

Confocal immunofluorescent analysis of MMP1 Antibody (Center) (Cat#TA324443) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

TA324443

Recombinant MMP1 was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-MMP1 antibody (clone F15P3B6). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.

TA327946

Human Placenta stained with anti-MMP-1 antibody.

TA353949

400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane and detected by immunoblotting with Mab 3B6 specific for MMP-1.

TA353335

IHC staining of human colon carcinoma.

TA353335

Tissues tested for MMP-1 expresstion using Mab 3B6.

TA353335

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM06648SU-N	MMP-1 antibody
	Mouse	IgG1	6A5	Ascites	Hu	E, ICC/IF, P, WB	0.1 ml / €430.00
							Acris Antibodies GmbH
+4 additional images	Highly validated antibody!
TA313099	MMP-1 antibody
	Rabbit polyclonal anti-MMP-1 antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA313100	MMP-1 antibody
	Rabbit polyclonal MMP1 (Cleaved-Phe100) antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA313101	MMP-1 antibody
	Rabbit polyclonal MMP1 (Cleaved-Pro269) antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA323954	MMP-1 antibody
	Anti-MMP1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €325.00
							OriGene Technologies, Inc.
+1 additional image
TA323955	MMP-1 antibody
	Anti-MMP1 rabbit polyclonal antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	P, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
+1 additional image
TA324443	MMP-1 (Center) antibody
	Rabbit polyclonal MMP1 Antibody (Center)
	Rabbit	Ig		Aff - Purified	Hu	ICC/IF, P, WB	0.4 ml / €325.00
							OriGene Technologies, Inc.
+2 additional images
TA326985	MMP-1 antibody
	MMP1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu	WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA327946	MMP-1 antibody
	Mouse Monoclonal anti-MMP1 Antibody
	Mouse	IgG1	F15P3B6	Aff - Purified	Hu	P, WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA347429	MMP-1 antibody
	Rabbit Polyclonal Anti-MMP1 Antibody
	Rabbit	IgG		Purified	Hu	WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA353335	MMP-1 antibody
	Mouse monoclonal Anti-MMP1 Clone 3B6
	Mouse	IgG1	3B6	Purified		ICC/IF, IP, P, WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
+2 additional images
TA353949	MMP-1 antibody
	Rabbit Polyclonal anti-MMP-1 Antibody
	Rabbit	IgG		Purified	Hu	P	0.1 ml / €325.00
							OriGene Technologies, Inc.
AF9110	MMP-1 (active) antibody
	Mouse	IgG2b	195-1H6	Purified	Hu	P, WB	0.1 mg / €300.00
							Acris Antibodies GmbH
AM08197HR-N	MMP-1 antibody
	Mouse	IgG2b	SB12e	HRP	Hu	C, E, P, WB	1 ml / €470.00
							Acris Antibodies GmbH
AM08197PU-N	MMP-1 antibody
	Mouse	IgG2b	SB12e	Purified	Hu	C, E, P, WB	0.1 mg / €300.00
							Acris Antibodies GmbH
AP06224PU-N	MMP-1 antibody
	Rabbit			Aff - Purified	Hu	ICC/IF, P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
AP08372PU-N	MMP-1 (hinge region) antibody
	Rabbit	IgG		Aff - Purified	Hu	P, WB	50 µg / €530.00
							Acris Antibodies GmbH
AP31184PU-N	MMP-1 (C-term) antibody
	Rabbit	IgG		Aff - Purified	Hu	P, WB	0.25 ml / €470.00
							Acris Antibodies GmbH
C0262-1	MMP-1 antibody
	Rabbit			Aff - Purified	Hu	E, WB	50 µg / €230.00
							Assay Biotech
C0262-2	MMP-1 antibody
	Rabbit			Aff - Purified	Hu	E, WB	0.1 mg / €330.00
							Assay Biotech