MMP-12 antibody

Principal name

MMP-12 antibody

Alternative names for MMP-12 antibody

Macrophage metalloelastase, HME, ME, Matrix metalloproteinase-12, MMP12, Macrophage elastase

SwissProt ID

P34960 (Mouse), P39900 (Human), P79227 (Rabit), Q63341 (Rat)

Gene ID

4321 (MMP12), 17381 (Mmp12)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Dot blot (Dot), Frozen Sections (C), ELISA (capture) (E(capture)), ELISA capture and detection (E(caption)/E(detection))

Background of MMP-12 antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis, metastasis, and atherosclerosis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.MMP12 was first described in murine macrophages, later in human macrophages, and more recently in other cell types. Also known as metalloelastase, MMP12 is able to degrade elastin, entactin, laminin 1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP12 is often confused with the Serine proteinase, Leukocyte elastase (EC 3.4.21.37) because of similar nomenclature. MMP12 is structurally similar to the classical MMPs (MMP1, MMP3); it contains a propeptide with autoinhibitory cysteine switch site, a well-conserved zinc site, hinge region and hemopexin domain. MMP12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kD, and is quickly activated to the 45 kD form; and this breaks down to cascade of active forms, ending with the common 22 kD form. Stimulated macrophages produce MMP12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-12 antibody

(0.5ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-12 antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide (25uM, 1hour), using MMP12 (Cleaved-Glu106) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-12 Control Peptide

MMP-12 Purified
50 µg / €190.00
  Acris Antibodies GmbH

MMP-12 antibody

Western blot analysis of MMP-12 expression in Mouse small intestine tissue lysate. Rabbit Aff - Purified Ms ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MMP-12 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-12 antibody

Immunohistochemistry (IHC) analyzes of MMP-12 antibody (Cat.-No.: AP20790PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P
0.1 mg / €350.00
  Acris Antibodies GmbH

MMP-12 antibody

Western Blot analysis of MMP12 expression in transfected 293T cell line (H00004321-T02) by MMP12 MaxPab polyclonal antibody.

Lane 1: MMP12 transfected lysate(54.00 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MMP-12 antibody

MMP-12 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MMP-12 antibody

MMP-12 Rabbit Serum Hu, Rt E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

MMP-12 antibody

Western Blot analysis of MMP12 expression in transfected 293T cell line (H00004321-T01) by MMP12 MaxPab polyclonal antibody.

Lane 1: MMP12 transfected lysate(54.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MMP-12 antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 polyclonal antibody ( Cat # PAB4781 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MMP-12 antibody

Western blot analysis of MMP12 expression in mouse small intestine tissue lysate. Rabbit Hu, Ms WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MMP-12 (cleaved) antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

MMP-12 (cleaved) antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

MMP-12 antibody

MMP-12 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

MMP-12 antibody

Western blot analysis of MMP12 expression in A549 (A); HEK293 (B); HeLa (C) whole cell lysates. Rabbit Aff - Purified Hu WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MMP-12 antibody

Western blot analysis of MMP12 expression in A549 (A); HEK293 (B); HeLa (C) whole cell lysates. Rabbit Aff - Purified Hu WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MMP-12 antibody

Western blot analysis of MMP12 expression in A549 (A); HEK293 (B); HeLa (C) whole cell lysates. Rabbit Aff - Purified Hu WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MMP-12 antibody

Human lung tissue sections Mouse IgG1 603E6.22 Biotin Hu C
50 µg / €360.00
  Dendritics SAS

MMP-12 antibody

Human lung tissue sections Mouse IgG1 603E6.22 Biotin Hu C
0.1 mg / €480.00
  Dendritics SAS

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