MMP-28 antibody

Principal name

MMP-28 antibody

Alternative names for MMP-28 antibody

Matrix metalloproteinase-28, MMP28, MMP25, UNQ1893/PRO4339, Epilysin

SwissProt ID

Q9H239 (Human)

Gene ID

79148 (MMP28)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MMP-28 antibody

Matrix Metalloproteinase 28, also known as epilysin, was first cloned from human skin keratinocytes and described to reflect its role in the remodeling of the epidermis. It was found in testis, as well as heart, brain, placenta, lung, prostate, intestine, and colon. MMP28 was later cloned from human lung and found in lung, kidney, brain, skeletal muscle, and several tumor cell lines. At least three MMP28 transcripts of 2.6, 2.0, and 1.2 kb have been reported possibly representing alternatively spliced forms of MMP28. There are two human sequences (isoform1 and isoform 2) which encode proteins of 520 and 393 amino acids with predicted masses of 58.9 and 44.5 kDa respectively. The mouse sequence contains two inserts of 41 and 39 amino acids respectively, relative to the human sequence. Mouse epilysin has a predicted molecular mass of approx. 70 kDa. Mouse and human epilysin are highly conserved and share 97% identical residues. Epilysin (MMP-28) contains the key domains of the other MMPs: a signal peptide, conserved cysteine-containing prodomain (with a furin cleavage site), conserved histidine-containing catalytic domain, hinge domain, and hemopexin domain. MMP28 has a furin cleavage site, similar to MMP11, and is cleaved by the prohormone convertase family of enzymes. Sequence identity between MMP28 and the other MMPs is low overall. MMP28 is most closely related to MMP19 (39% sequence identity). Recombinant MMP28 degrades casein. EDTA and batimastat (selective MMP inhibitor) inhibits the proteolytic activity of epilysin. MMP28 is expressed in tumors as well as normal adult and fetal tissues. It is involved in homeostasis, wound repair, and cell proliferation (during epithelial repair). The MMP28 gene maps to chromosome 17q11.2 and includes 8 exons and 7 introns.

General readings

Marchenko G.N., et al. (2001) Gene. 265:87-93
Lohi J., et al. (2001) J. Biol. Chem. 276:10134-10144

17 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-28 (298-312) antibody

Anti-MMP28 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

MMP-28 antibody

Predicted band size: 44 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-28 antibody

Predicted band size: 44 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 50 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-28 antibody

Host: Rabbit; Target Name: MMP28; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MMP-28 antibody

MMP-28 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MMP-28 antibody

Western Blot analysis of MMP28 expression in transfected 293T cell line (H00079148-T01) by MMP28 MaxPab polyclonal antibody.

Lane 1: MMP28 transfected lysate(43.23 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MMP-28 antibody

MMP-28 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MMP-28 antibody

Western Blot analysis of MMP28 expression in transfected 293T cell line (H00079148-T02) by MMP28 MaxPab polyclonal antibody.

Lane 1: MMP28 transfected lysate(14.3 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MMP-28 antibody

Western Blot analysis of MMP28 expression in transfected 293T cell line (H00079148-T03) by MMP28 MaxPab polyclonal antibody.

Lane 1: MMP28 transfected lysate(43.23 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MMP-28 antibody

MMP-28 Mouse IgG2a 3C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MMP-28 antibody

Immunofluorescence: MMP28 Antibody [NBP1-33768] - Immunofluorescence analysis of methanol-fixed HeLa, using MMP28(NBP1-33768) antibody at 1:200 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MMP-28 (123-520, His-tag)

MMP-28 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

MMP-28 (123-520, His-tag)

MMP-28 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-28

MMP-28 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MMP-28

MMP-28 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MMP28 293T Cell Transient Overexpression Lysate(Denatured)

MMP28 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

MMP28 293T Cell Transient Overexpression Lysate(Denatured)

MMP28 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

17 Item(s)

per page
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