MMP-3 antibody

Principal name

MMP-3 antibody

Alternative names for MMP-3 antibody

Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP3, STMY1

SwissProt ID

P03957 (Rat), P08254 (Human), P28862 (Mouse), P28863 (Rabit)

Gene ID

4314 (MMP3), 17392 (Mmp3), 171045

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Can (Canine), Por (Porcine), Eq (Equine), Mky (Monkey), Bov (Bovine), GP (Guinea Pig), Sh (Sheep), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit, Rat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of MMP-3 antibody

Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures.1 MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.2 The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.

General readings

Ito, A. et al. Identification of rabbit uterine cervical procollagenase activator as rabbit matrix metalloproteinase 3 (stromelysin). Comp. Biochem. Physiol., 99B. 381-385, 1991.
Morita, Y. et al. A two-step sandwich enzyme immunoassay for rabbit proMMP-3 using monoclonal antibodies. Connect. Tissue. 27. 183-190, 1995 (In Japanese).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-3 antibody

Immunohistochemistry (IHC) analyzes of MMP-3 antibody Cat.-No AP06233PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MMP-3 antibody

MMP3 antibody - middle region validated by WB using Articular cartilage secretome at 1: 1000. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Sample Type: Equine Cartilage Explants; Primary Dilution: 1: 1000; Secondary: Abcam ab97200 (Dilution: 1: 20, 000) Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MMP3 Rabbit Polyclonal antibody. (TA590687) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MMP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806871) Mouse IgG2b OTI1E9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMP-3 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MMP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806979) Mouse IgG1 OTI2D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMP-3 (1-35) antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

MMP-3 antibody

Human colorectal cancer; . Application: IHC. Species+tissue/cell type: Control-Human small intestine, Sample-human colorectal cancer. Primary antibody dilution: 1:100. Secondary antibody: Biotinylated pig anti-rabbit+streptavidin-HRP; MMP3 antibody - middle region (ARP42042_P050) in Human colorectal cancer cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MMP-3 (459-473) antibody

(0.2ug/ml) staining of Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Western blot analysis of extracts from 293 cells, using MMP-3 antibody (#TA313102).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Western blot analysis of extracts from A549 cells and HeLa cells treated with etoposide (25uM, 24hours), 293 cells treated with etoposide (25uM, 1hour), using MMP3 (Cleaved-Phe100) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer and breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using MMP3 antibody at dilution 1/10, on the right is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MMP3 antibody at dilution 1/10. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Western blot analysis of A549 cell lysate using MMP3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Western blot analysis of recombinant human MMP-3 protein using anti-MMP-3, clone F36P1B4. Mouse IgG1 F36P1B4 Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Western Blot: MMP3 Antibody [TA336495] - Analysis of MMP-3 (S453) antibody  in extracts from 293 cells. Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Western blot analysis on 293 cell lysate using MMP3 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane and detected by immunoblotting with Mab 1B4 specific for MMP-3. Mouse IgG1 1B4 Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MMP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806869) Mouse IgG1 OTI2H6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMP-3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP3 (RC210235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP3. Mouse IgG1 OTI4B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MMP-3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP3 (RC210235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP3. Mouse IgG1 OTI2D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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