MMP-9 antibody

Principal name

MMP-9 antibody

Alternative names for MMP-9 antibody

Matrix metalloproteinase-9, MMP9, 92 kDa type IV collagenase, 92 kDa gelatinase, GELB, Gelatinase B, CLG4B

SwissProt ID

P14780 (Human), P41245 (Mouse), P41246 (Rabit), P50282 (Rat), P52176 (Bovin)

Gene ID

4318 (MMP9), 17395 (Mmp9), 81687

Available reactivities

GP (Guinea Pig), Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Marmoset, Hst (Hamster), Mky (Monkey)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP)

Background of MMP-9 antibody

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins . They are secreted as zymogens (Pro-MMPs) that are activated by a variety of proteinases or by reaction with organic mercurials. They are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs) and by 2-macroglobulin . The regulation of MMP activity is important in tissue remodeling, inflammation, tumor growth and metastasis .

Human MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen . Cleavage of Pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa . MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain . Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases . MMP-9 is inhibited by.2-macroglobulin or by TIMP-1 , which binds to Pro-MMP-9 as well as to active MMP-9 . In vitro treatment of Pro-MMP-9 with 4-aminophenylmercuric acid (APMA) produces not only the 82 kDa active enzyme but also a C-terminal truncated form of approximately 65 kDa with the activity comparable to that of the 82 kDa form .

Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells(1, 7- 21). Pro-MMP-9 expression is upregulated by TGF-.1, IL-1., TGF-., PDGF-AB, TNF-., and IL-1. . Substrates for MMP-9 include denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1., and entactin, a molecule that bridges laminin and type IV collagen .

General readings

Wilhelm, S.M. et al. (1989) J. Biol. Chem. 264:17213.

Matrisian, L.M. (1992) BioEssays 14:455.

Birkedal-Hansen, H. et al. (1993) Crit. Rev. Oral Biol. Med. 4:197.

Birkedal-Hansen, H. (1995) Curr. Opin. Cell Biol. 7:728.

Ogata, Y. et al. (1992) J. Biol. Chem. 267:3581.

Sires, U.I. et al. (1993) J. Biol. Chem. 268:2069.

Lyons, J.G. et al. (1993) J. Biol. Chem. 268:19143.

Okada, Y. et al. (1995) Lab. Invest. 72:311.

Tamura, T. et al. (1996) Endocrinology 137:3729.

Okamoto, T. et al. (1997) J. Biol. Chem. 272:6059.

O’Connell, J.P. et al. (1994) J. Biol. Chem. 269:14967.

Koide, H. et al. (1996) Am. J. Kidney Dis. 28:32.

Hirose, T. et al. (1992) J. Rheumatol. 19:593.

Iwata, H. et al. (1996) Jpn. J. Cancer Res. 87:602.

Cullen, B. et al. (1997) Int. J. Biochem. Cell Biol. 29:241.

Guerin, C.W. and P.C. Holland (1995) Dev. Dyn. 202:91.

Hanemaaijer, R. et al. (1993) Biochem. J. 296:803.

Maeda, A. and R.A. Sobel (1996) J. Neuropathol. Exp. Neurol. 55:300.

Gress, T.M. et al. (1995) Int. J. Cancer 62:407.

Ueda, Y. et al. (1996) Am. J. Pathol. 148:611.

Lyons, J.G. et al. (1991) Biochemistry 30:1449.

Imai, K. et al. (1994) FEBS Lett. 369:249.

Ito, A. et al. (1996) J. Biol. Chem. 271:14657.

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (2)
  4. Overnight Delivery (2)

Items 1 to 20 of 80 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-9 antibody

Staining of Human prostatic adenocarcinoma with MMP-9 Antibody Cat.-No AF6910 (Clone 56-2A4) at 2 µg/ml. Antibody positive in cytoplasma and in lumen secreted. 40x Mouse IgG1 56-2A4 Purified Hu C, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-9 antibody

Formalin-Fixed, Paraffin-Embedded Human ovarian carcinoma stained with MMP9 antibody Cat.-No. AM10115SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of carcinoma cells. Rabbit IgG EP1255Y Supernatant GP, Hu ICC/IF, P, WB
1 ml / €600.00
  Acris Antibodies GmbH

MMP-9 antibody

Immunohistochemical analysis of paraffin-embedded brain tissues using MMP9 mouse mAb with DAB staining. Mouse IgG2a 5G3 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MMP-9 antibody

Western blot analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa) Mouse IgG2a 5C3 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MMP-9 antibody

  Rabbit polyclonal anti-MMP-9 antibody  
Western blot analysis of extracts from HeLa cells, using MMP-9 antibody (#TA312179).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit polyclonal anti-MMP-9 antibody  
Western blot analysis of MMP-9 in the supernatant of rat MMP transfected cells. Rabbit Purified Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal MMP-9 Antibody  
Western blot analysis on HepG2 cell lysate using MMP9 Antibody Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Mouse Monoclonal Anti-MMP9 Antibody  
MMP-9 Mouse IgG2a S51-82 Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal MMP9 Antibody  
Western blot analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at  1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit anti-MMP9 Polyclonal Antibody  
Western blot analysis of extracts of mouse spinal cord cell lines, using MMP9 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Mouse Monoclonal MMP-9 Antibody (4A3)  
Western Blot: MMP9 Antibody (4A3) [TA336901] - Western blot analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times. Mouse IgG1 4A3 Purified Hu, Rt C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP9 Antibody (Internal)  
Anti-MMP9 antibody IHC of human skin, granulation tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Can, Hst, Hu, Mky, Rb
50 µg / €379.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP9 Antibody (Internal)  
Anti-MMP9 antibody IHC of human skin, granulation tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP-9 Antibody  
Western blot analysis on HepG2 cell lysate using MMP9 antibody, the lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP9 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (MMP9 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP9 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: K562 cells, Jurkat cells, hepg2 cells, Primary antibody: (MMP9 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP9 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (MMP9 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Mouse monoclonal Anti-MMP9 Clone 2C3  
400ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane and detected by immunoblotting with mAb 2C3 specific to MMP-9. Mouse IgG1 2C3 Purified Hu ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Mouse monoclonal Anti-a-MMP9 Clone 4A3  
Western Bolt application of mAb 4A3 Mouse IgG1 4A3 Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal anti-MMP-9 (92kDa Collagenase IV) Antibody  
Human Placenta stained with anti-MMP-9 (92kDa Collagenase IV) antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Items 1 to 20 of 80 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn