MMP-9 antibody

Principal name

MMP-9 antibody

Alternative names for MMP-9 antibody

Matrix metalloproteinase-9, MMP9, 92 kDa type IV collagenase, 92 kDa gelatinase, GELB, Gelatinase B, CLG4B

SwissProt ID

P14780 (Human), P41245 (Mouse), P41246 (Rabit), P50282 (Rat), P52176 (Bovin)

Gene ID

4318 (MMP9), 17395 (Mmp9), 81687

Available reactivities

GP (Guinea Pig), Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Marmoset, Hst (Hamster), Mky (Monkey)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP)

Background of MMP-9 antibody

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins . They are secreted as zymogens (Pro-MMPs) that are activated by a variety of proteinases or by reaction with organic mercurials. They are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs) and by 2-macroglobulin . The regulation of MMP activity is important in tissue remodeling, inflammation, tumor growth and metastasis .

Human MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen . Cleavage of Pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa . MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain . Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases . MMP-9 is inhibited by.2-macroglobulin or by TIMP-1 , which binds to Pro-MMP-9 as well as to active MMP-9 . In vitro treatment of Pro-MMP-9 with 4-aminophenylmercuric acid (APMA) produces not only the 82 kDa active enzyme but also a C-terminal truncated form of approximately 65 kDa with the activity comparable to that of the 82 kDa form .

Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells(1, 7- 21). Pro-MMP-9 expression is upregulated by TGF-.1, IL-1., TGF-., PDGF-AB, TNF-., and IL-1. . Substrates for MMP-9 include denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1., and entactin, a molecule that bridges laminin and type IV collagen .

General readings

Wilhelm, S.M. et al. (1989) J. Biol. Chem. 264:17213.

Matrisian, L.M. (1992) BioEssays 14:455.

Birkedal-Hansen, H. et al. (1993) Crit. Rev. Oral Biol. Med. 4:197.

Birkedal-Hansen, H. (1995) Curr. Opin. Cell Biol. 7:728.

Ogata, Y. et al. (1992) J. Biol. Chem. 267:3581.

Sires, U.I. et al. (1993) J. Biol. Chem. 268:2069.

Lyons, J.G. et al. (1993) J. Biol. Chem. 268:19143.

Okada, Y. et al. (1995) Lab. Invest. 72:311.

Tamura, T. et al. (1996) Endocrinology 137:3729.

Okamoto, T. et al. (1997) J. Biol. Chem. 272:6059.

O’Connell, J.P. et al. (1994) J. Biol. Chem. 269:14967.

Koide, H. et al. (1996) Am. J. Kidney Dis. 28:32.

Hirose, T. et al. (1992) J. Rheumatol. 19:593.

Iwata, H. et al. (1996) Jpn. J. Cancer Res. 87:602.

Cullen, B. et al. (1997) Int. J. Biochem. Cell Biol. 29:241.

Guerin, C.W. and P.C. Holland (1995) Dev. Dyn. 202:91.

Hanemaaijer, R. et al. (1993) Biochem. J. 296:803.

Maeda, A. and R.A. Sobel (1996) J. Neuropathol. Exp. Neurol. 55:300.

Gress, T.M. et al. (1995) Int. J. Cancer 62:407.

Ueda, Y. et al. (1996) Am. J. Pathol. 148:611.

Lyons, J.G. et al. (1991) Biochemistry 30:1449.

Imai, K. et al. (1994) FEBS Lett. 369:249.

Ito, A. et al. (1996) J. Biol. Chem. 271:14657.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-9 antibody

  Mouse Monoclonal Anti-MMP9 Antibody  
MMP-9 Mouse IgG2a S51-82 Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €410.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal MMP9 Antibody  
Western blot analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at  1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €371.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit anti-MMP9 Polyclonal Antibody  
Western blot analysis of extracts of mouse spinal cord cell lines, using MMP9 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP-9 Antibody  
Western blot analysis on HepG2 cell lysate using MMP9 antibody, the lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MMP-9 antibody

  Rabbit Polyclonal Anti-MMP9 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (MMP9 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

MMP-9 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG2a L51/82 Supernatant Hu, Ms, Rt C, IP, WB
5 ml / €460.00
  Antibodies Incorporated

NeuroMab

MMP-9 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG2a L51/82 Purified Hu, Ms, Rt C, IP, WB
0.1 ml / €540.00
  Antibodies Incorporated

NeuroMab

MMP-9 (C-term) antibody

TOP: Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 
BUTTOM: Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  OriGene Technologies GmbH

MMP-9 (C-term) antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with MMP9 antibody AP22782PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms P, WB
50 µg / €380.00
  OriGene Technologies GmbH

MMP-9 antibody

MMP-9 Goat Aff - Purified Ms, Rt C, E, IP, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

ELISA Kits

Catalog No.    

Mouse MMP-9 ELISA Kit

Mouse MMP-9 ELISA Kit  
1 kit / €542.00
  OriGene Technologies, Inc.

11 Item(s)

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