MNAT1 / RNF66 antibody

Principal name

MNAT1 / RNF66 antibody

Alternative names for MNAT1 / RNF66 antibody

RING finger protein MAT1, RING finger protein 66, Menage a trois, CAP35, MAT1, CDK-activating kinase assembly factor MAT1, CDK7/cyclin H assembly factor, Cyclin G1-interacting protein

SwissProt ID

P51948 (Human), P51949 (Mouse), P51951 (Xenla)

Gene ID

4331 (MNAT1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Dros (Drosophila), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of MNAT1 / RNF66 antibody

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.Cyclin-dependent kinases (CDKs), which play an essential role in cell cycle control of eukaryotic cells, are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 (MIM 601955), cyclin H (CCNH; MIM 601953), and MAT1. MAT1 (for 'menage a trois-1') is involved in the assembly of the CAK complex.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-498 AA053721.1 1-498 499-1372 BC000820.1 489-1362 1373-1388 BU620200.1 1-16 c Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human MNAT1 with an internal ID of P02090

General readings

Zhou,M., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (22), 12666-12671

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MNAT1 / RNF66 antibody

Western blot analysis of extracts from HeLa cells, HepG2 cells and 293 cells, using MAT1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MNAT1 / RNF66 antibody

Predicted band size: 36 kDa . Positive control: A431 and HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A431 cell lysate Lane2: HeLa cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MNAT1 / RNF66 antibody

Western blot analysis of extracts of various cell lines, using MNAT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MNAT1 / RNF66 antibody

WB Suggested Anti-MNAT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 721_B cell lysateMNAT1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MNAT1 / RNF66 antibody

WB Suggested Anti-MNAT1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate. MNAT1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MNAT1 / RNF66 antibody

Western blot(WB) analysis of Mat1 antibody (Cat.-No.: AP06703PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MNAT1 / RNF66 antibody

Western blot analysis of extracts from HeLa/HepG2/293 cells, using MAT1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

MNAT1 / RNF66 antibody

Western blot analysis of extracts from HeLa/HepG2/293 cells, using MAT1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

MNAT1 / RNF66 antibody

Human Jurkat; WB Suggested Anti-MNAT1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. MNAT1 is supported by BioGPS gene expression data to be expressed in Jurkat.; MNAT1 antibody - C-terminal region (ARP38150_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

MNAT1 / RNF66 antibody

Human 721_B; WB Suggested Anti-MNAT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. MNAT1 is supported by BioGPS gene expression data to be expressed in 721_B.; MNAT1 antibody - N-terminal region (P100992_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

MNAT1 / RNF66 antibody

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MNAT1 / RNF66 antibody

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MNAT1 / RNF66 antibody

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MNAT1 / RNF66 antibody

MNAT1 / RNF66 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

MNAT1 / RNF66 antibody

MNAT1 / RNF66 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

MNAT1 / RNF66 antibody

Immunohistochemistry-Paraffin: MNAT1 Antibody [NBP1-81257] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MNAT1 / RNF66

MNAT1 / RNF66 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MNAT1 / RNF66

MNAT1 / RNF66 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MNAT1 / RNF66

MNAT1 / RNF66 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

MNAT1 / RNF66

MNAT1 / RNF66 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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