MNDA antibody

Principal name

MNDA antibody

Alternative names for MNDA antibody

Myeloid cell nuclear differentiation antigen

SwissProt ID

P41218 (Human)

Gene ID

4332 (MNDA)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MNDA antibody

The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage.MNDA may act as a transcriptional activator/repressor in the myeloid lineage. It plays a role in the granulocyte/monocyte cell-specific response to interferon. MNDA stimulates the DNA binding of the transcriptional repressor protein YY1.The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Chow, S., et al., Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations. Cytometry A, 2005. 67(1): p. 4-17.
2. Mondini, M., et al., Role of the interferon-inducible gene IFI16 in the etiopathogenesis of systemic autoimmune disorders. Ann N Y Acad Sci, 2007. 1110: p. 47-56.
3. Asefa, B., et al., The interferon-inducible p200 family of proteins: a perspective on their roles in cell cycle regulation and differentiation. Blood Cells Mol Dis, 2004. 32(1): p. 155-67.
4. Xie, J., J.A. Briggs, and R.C. Briggs, Human hematopoietic cell specific nuclear protein MNDA interacts with the multifunctional transcription factor YY1 and stimulates YY1 DNA binding. J Cell Biochem, 1998. 70(4): p. 489-506.
5. Miranda, R.N., et al., Immunocytochemical analysis of MNDA in tissue sections and sorted normal bone marrow cells documents expression only in maturing normal and neoplastic myelomonocytic cells and a subset of normal and neoplastic B lymphocytes. Hum Pathol, 1999. 30(9): p. 1040-9.
6. Xie, J., et al., MNDA binds NPM/B23 and the NPM-MLF1 chimera generated by the t (3; 5) associated with myelodysplastic syndrome and acute myeloid leukemia. Exp Hematol, 1997. 25(11): p. 1111-7.
7. Choubey, D. and R. Panchanathan, Interferon-inducible Ifi200-family genes in systemic lupus erythematosus. Immunol Lett, 2008. 119(1-2): p. 32-41.
8. Choubey, D., R. Deka, and S.M. Ho, Interferon-inducible IFI16 protein in human cancers and autoimmune diseases. Front Biosci, 2008. 13: p. 598-608.
9. Johnstone, R.W., J.A. Kerry, and J.A. Trapani, The human interferon-inducible protein, IFI 16, is a repressor of transcription. J Biol Chem, 1998. 273(27): p. 17172-7.
10. Briggs, R., et al., Interferon alpha selectively affects expression of the human myeloid cell nuclear differentiation antigen in late stage cells in the monocytic but not the granulocytic lineage. J Cell Biochem, 1994. 54(2): p. 198-206.
11. Briggs, J.A., et al., Cloning and expression of the human myeloid cell nuclear differentiation antigen: regulation by interferon alpha. J Cell
Biochem, 1992. 49(1): p. 82-92.
12. Kanellis, G., et al., Identification of MNDA as a new marker for nodal marginal zone lymphoma. Leukemia, 2009.
13. Briggs, R.C., et al., Dysregulated human myeloid nuclear differentiation antigen expression in myelodysplastic syndromes: evidence for a role in apoptosis. Cancer Res, 2006. 66(9): p. 4645-51.
14. Pradhan, A., et al., Differentially expressed genes in adult familial myelodysplastic syndromes. Leukemia, 2004. 18(3): p. 449-59.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MNDA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MNDA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807178) Mouse IgG2a OTI4H6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MNDA antibody

Western blot analysis of extracts from LOVO cells, using MNDA antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MNDA antibody

WB Suggested Anti-MNDA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MNDA antibody

Western blot (WB) analysis of MNDA antibody (Cat.-No.: AP01484PU-N) in extracts from LOVO cells. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MNDA (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with MNDA Antibody (N-term) Cat.-No AP52716PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MNDA antibody

Immunoperoxidase of purified MaxPab antibody to MNDA on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MNDA antibody

Immunoperoxidase of monoclonal antibody to MNDA on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse IgG2a 1H2 Purified Hu E, P
0.1 mg / €370.00
  Abnova Taiwan Corp.

MNDA antibody

Western blot analysis of extracts from LOVO cells, using MNDA Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

MNDA antibody

Western blot analysis of extracts from LOVO cells, using MNDA Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

MNDA antibody

Transfected 293T; WB Suggested Anti-MNDA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; MNDA antibody - C-terminal region (ARP38152_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MNDA antibody

Immunohistochemical analysis of MNDA staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MNDA antibody

Immunohistochemical analysis of MNDA staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MNDA antibody

Immunohistochemical analysis of MNDA staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MNDA antibody

Western Blot: MNDA Antibody [NBP1-00929] - Western blot (WB) analysis of MNDA (V406) antibody in extracts from LOVO cells. Rabbit Aff - Purified Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

MNDA antibody

MNDA Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

MNDA antibody

MNDA Rabbit IgG Aff - Purified Hu E, WB
50 µg / €290.00
  Novus Biologicals Inc.

MNDA antibody

Immunohistochemistry-Paraffin: MNDA Antibody [NBP1-87180] - Immunohistochemical staining of human bone marrow shows strong nuclear positivity in subsets of bone marrow poietic cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MNDA

MNDA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MNDA (1-407, His-tag)

MNDA Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,500.00
  Acris Antibodies GmbH

MNDA (1-407, His-tag)

MNDA Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €520.00
  Acris Antibodies GmbH

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