MPG / ANPG antibody

Principal name

MPG / ANPG antibody

Alternative names for MPG / ANPG antibody

DNA-3-methyladenine glycosylase, AAG, ADPG, MID1, 3-methyladenine DNA glycosidase, 3-alkyladenine DNA glycosylase, N-methylpurine-DNA glycosylase

SwissProt ID

P23571 (Rat), P29372 (Human), Q04841 (Mouse)

Gene ID

4350 (MPG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of MPG / ANPG antibody

MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

General readings

Samson L, Derfler B, Boosalis M, Call K.
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16.
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9127-31.
PMID: 1924375

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MPG / ANPG antibody

Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse IgG2a 1E10 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MPG / ANPG antibody

Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml] Mouse IgG2a 2C5 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MPG / ANPG (C-term) antibody

TA302936 staining (2µg/ml) of K562 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MPG / ANPG antibody

Western blot analysis of extracts of various cell lines, using MPG antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MPG / ANPG antibody

WB Suggested Anti-MPG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MPG / ANPG antibody

WB Suggested Anti-MPG Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MPG / ANPG antibody

MPG MaxPab rabbit polyclonal antibody. Western Blot analysis of MPG expression in human colon. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MPG / ANPG antibody

Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 0.2 ug/ml] Mouse IgG2a 2D2 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MPG / ANPG antibody

Immunofluorescence of monoclonal antibody to MPG on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 4B12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MPG / ANPG antibody

Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1 ug/ml] Mouse IgG2b 1G6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MPG / ANPG antibody

MPG polyclonal antibody ( Cat # PAB6607 ) staining ( 2 µg/ml ) of K-562 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MPG / ANPG antibody

Human Jurkat; WB Suggested Anti-MPG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; MPG antibody - middle region (ARP44693_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MPG / ANPG antibody

MPG / ANPG Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

MPG / ANPG antibody

Western blot analysis of MPG expression in Jurkat (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MPG / ANPG antibody

Western blot analysis of MPG expression in Jurkat (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MPG / ANPG antibody

Western blot analysis of MPG expression in Jurkat (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MPG / ANPG antibody

Western Blot: MPG Antibody [NB300-870] - NB300-870 staining (1µg/ml) of HEK293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MPG / ANPG antibody

Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787] - Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MPG / ANPG (1-298, His-tag)

MPG / ANPG Human Purified > 90 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

MPG / ANPG (1-298, His-tag)

MPG / ANPG Human Purified > 90 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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