MRE11 antibody

Principal name

MRE11 antibody

Alternative names for MRE11 antibody

MRE11A, HNGS1, MRE11 homolog 1, Double-strand break repair protein MRE11A, MRE11 meiotic recombination 11 homolog A

SwissProt ID

P32829 (Yeast), P49959 (Human), Q61216 (Mouse), Q9IAM7 (Chick), Q9JIM0 (Rat), Q9W6K1 (Xenla)

Gene ID

4361 (MRE11A), 17535 (Mre11a), 855264 (MRE11), 2543611 (aar2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Macaque, Ye (Yeast), Hst (Hamster), S. cerevisiae (Saccharomyces cerevisiae), Can (Canine), Chk (Chicken), Mky (Monkey), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Frozen Sections (C)

Background of MRE11 antibody

DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MRE11 antibody

Western Blot analysis of Mre11 on 50 ug of HeLa and MEF lysates Rabbit IgG Purified Hu, Ms ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

MRE11 antibody

Western blot analysis of MRE11 in rat lung tissue lysate with MRE11 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRE11 (68-706) antibody

WB using Anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4?. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature. Rabbit IgG Aff - Purified Can, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRE11 (578-590) antibody

ChIP using Mre11 (S. cerevisiae) antibody. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). Rabbit IgG Aff - Purified S. cerevisiae ChIP, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRE11 antibody

Western blot analysis of extracts of various cell lines, using MRE11A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRE11 antibody

WB Suggested Anti-MRE11A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 721_B cell lysateMRE11A is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MRE11 antibody

WB Suggested Anti-MRE11A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MRE11 antibody

Western blot analysis of MRE11A expression in LOVO cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRE11 antibody

Western blot analysis of MRE11 Antibody Cat.-No AP06803PU-N in extracts from RAW264.7 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MRE11 (68-706) antibody

Immunohistochemistry Image: Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MRE11 antibody

Immunohistochemistry analyzes of MRE11 antibody (Cat.-No.: AP20369PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MRE11 Control Peptide

MRE11
50 µg / €160.00
  Acris Antibodies GmbH

MRE11 antibody

Detection of human Mre11 in whole cell lysates of HeLa cells by Western blotting using Rabbit anti Human Mre11 (SP2110P) Rabbit Aff - Purified Hu WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MRE11 antibody

MRE11A monoclonal antibody (M01), clone 1D8-A6 Western Blot analysis of MRE11A expression in HL-60 ( Cat # L014V1 ). Mouse IgG2a 1D8-A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MRE11 antibody

MRE11 Mouse IgG2a 3H4-F4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MRE11 antibody

Western blot analysis for MRE11A in 293 cells : 10 ug per lane 293 cell lysate was resolved by SDS-PAGE and the blot was probed with 2 µg/ml MRE11A polyclonal antibody ( Cat # PAB0179 ) . A protein band of approximate molecular weight of 81 kDa is detected. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MRE11 antibody

Western blot using Mre11a polyclonal antibody ( Cat # PAB10276 ) shows detection of a band ~ 80 kDa corresponding to mouse Mre11a ( arrowhead ).
Lanes 1-4 contain 0.5 µg, 0.3 µg, 0.1 µg and 0.05 µg of purified mouse Mre11a protein, respectively.
After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C.
The membrane was then washed and reacted with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Hu, Macaque, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MRE11 antibody

Chromatin Immunoprecipitation ( ChIP ) using MRE11 polyclonal antibody ( Cat # PAB10277 ) .
A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter ( uninduced 0 m lanes ) was shifted into galactose containing medium ( induced 60 m lanes ) .
After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions.
Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies.
The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break ( lower arrow ) and 67 kb from the break ( upper band, control locus ) .
Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD. Rabbit Ye ChIP, E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MRE11 antibody

Staining of MRE11A in the human epidermis using MRE11A polyclonal antibody ( Cat # PAB12381 ). Rabbit Hst, Hu, Ms C, ICC/IF, IP, WB
50 µl / €470.00
  Abnova Taiwan Corp.

MRE11 (249-264) antibody

The BCN antibody recognizes MRE11A, and its decrease in the presence of siRNA. Whole cell extracts (30 μg) from HEK-293 cells with or without siRNA where fractionated in a 8% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti-WRN BCN antibody diluted 1:100. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €320.00
  Antibody BCN

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