MRP8 / MRP14 antibody

Principal name

MRP8 / MRP14 antibody

Alternative names for MRP8 / MRP14 antibody

S100A8/A9, S100A8, S100A9, MRP8/14

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Can (Canine), Eq (Equine), Fe (Feline), GP (Guinea Pig), Mky (Monkey), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of MRP8 / MRP14 antibody

Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

General readings

1. Brandtzaeg, P., et al (1988). MAC387 antibody and detection of formalin resistant myelomonocytic L1 antigen J. Clin. Path. 41: 963-970.
2. Brandtzaeg, P. et al (1992). The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathol. 21: 191-196.
3. Flavell, D.J., Jones, D.B., Wright, D.H. (1987). Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35: 1217-1226.
4. Gutierrez, M. et al. (1999). The detection of CD2+, CD4+, CD8+ and WC1+, Tlymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using range of antigen recovery and signal amplification techniques.
Vet. Immunol. Immunopathol. 71: 321-334.
5. Ramsay, A. D. et al. (1991). Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies. Journal of Pathology. 164: 321 - 328.
6. Christgau, M. et al. (1998). Characterization of Immunocompetent cells in the diseased canine periodontium. Journal of Histochemistry & Cytochemistry. 46 (12): 1443-1454.
7. Perez, J. et al. (1999). Immunohistochemical study of the inflammatory infiltrate assocated with equine squamous cell carcinoma. J. Comp. Path. 121: 385-397.
8. Obert, L. et al. (2002). Early pathogenesis of transmucosal Feline Immunodeficiency Virus infection. J. Virol. 76 (12): 6311-6322.
9. Malik, N. et al. (1998). Apoptosis and Cell proliferation after porcine coronary angioplasty. Circulation. 98: 1657-1665.
10. Bagavant, H. et al. (2002). Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)). Am. J. Pathol. 160: 141-149.
11. Sanchez, J. et al. (2011) Microscopical and Immunological Features of Tuberculoid Granulomata and Cavitary Pulmonary Tuberculosis in Naturally Infected Goats. J Comp Pathol. Feb 17. [Epub ahead of print]
12. Pomeroy, I.M. et al. (2005) Demyelinated neocortical lesions in marmoset autoimmune ecephalomyelitis mimic those in multiple sclerosis. Brain. 128: 2713-21.
13. Vranckx, K. et al. (2012) Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain BMC Veterinary Research 8:24
14. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation J Clin Pathol 3 sept [epub ahead of print]
15. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama). Vet Immunol Immunopathol. 149: 66-75.
16. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells. Cell Medicine. 4: 1-11.
17. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae. PLoS Negl Trop Dis. 2013 Mar;7(3): e2138.
18. Tellez, A. et al. (2014) Experimental evaluation of efficacy and healing response of everolimus-eluting stents in the familial hypercholesterolemic swine model: a comparative study of bioabsorbable versus durable polymer stent platforms Coron Artery Dis. Mar 17. [Epub ahead of print]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MRP8/14 (S100A8/A9) antibody

Human Tonsil Paraffin Section stained with S100A8/9 Antibody Cat.-No BM4025 (Clone 27E10). Mouse IgG1 27E10 Purified Hu C, E, F, P, WB
0.1 mg / €500.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Human Tonsil Frozen Section stained with Biotin conjugated S100A8/9 Antibody Cat.-No  BM4025B (Clone 27E10). Mouse IgG1 27E10 Biotin Hu C, E, P
0.1 mg / €530.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Mouse IgG1 27E10 FITC Hu F, ICC/IF
0.2 mg / €550.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Human Tonsil Frozen Section stained with S100A8/9 Antibody Cat.-No  BM4025 (Clone 27E10). Mouse IgG1 27E10 Purified Hu C, E, P
1 mg / €780.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Mouse IgG1 FP 72 Purified Hu E, P
0.1 mg / €440.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Mouse IgG1 FP 72 Purified Hu E, P
10 µg / €170.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Immunofluorescence image of Calprotectin staining of Paraffin section of Human colon carcinoma. The sections were incubated with Calprotectin Antibody Cat.-No AM31907PU (1/500), followed by Goat-anti Mouse antibody coupled to Cy3. Nuclei stained with DAPI.  A: In Normal colon tissue, this antibody AM31907PU stains few cells in a blood vessel. B: Tumor tissue. Original magnification: x40 Mouse IgG1 FP 64 Purified Hu E, ICC/IF, P
0.1 mg / €440.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Immunofluorescence image of Calprotectin staining of Paraffin section of Human colon carcinoma. The sections were incubated with Calprotectin Antibody Cat.-No AM31907PU (1/500), followed by Goat-anti Mouse antibody coupled to Cy3. Nuclei stained with DAPI.  A: In Normal colon tissue, this antibody AM31907PU stains few cells in a blood vessel. B: Tumor tissue. Original magnification: x40 Mouse IgG1 FP 64 Purified Hu E, ICC/IF, P
10 µg / €170.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Mouse IgG1 FP 65 Purified Hu E, P
0.1 mg / €440.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Mouse IgG1 FP 65 Purified Hu E, P
10 µg / €170.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Immunofluorescence Image of Calprotectin staining of Paraffin Section of Human colon carcinoma. The section was incubated with AP02119SU at 1/500 dilution followed by incubation with Cy3-labeled Goat anti Rabbit antibody. 
Figure A: AP02119SU staining of colon carcinoma cells about to infiltrate a blood vessel. 
Figure B: Healthy Villi tissue remains unstained expect for a few Macrophages. 
Original magnification: x20 Rabbit Serum Hu E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Immunofluorescence Image of Calprotectin staining of Paraffin Section of Human colon carcinoma. The section was incubated with AP02119SU at 1/500 dilution followed by incubation with Cy3-labeled Goat anti Rabbit antibody. 
Figure A: AP02119SU staining of colon carcinoma cells about to infiltrate a blood vessel. 
Figure B: Healthy Villi tissue remains unstained expect for a few Macrophages. 
Original magnification: x20 Rabbit Serum Hu E, P, WB
20 µl / €170.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

BP4011 S100A8/A9 antibody staining of Human Skin Frozen Section. Chicken Aff - Purified Bov, Hu, Por C, E, P
0.2 mg / €390.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

Immunohistochemistry on paraffin section: Staining of human spleen with BP4011B Chicken Biotin Bov, Hu, Por C, E, P
0.2 mg / €400.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Chicken HRP Bov, Hu, Por E
0.1 ml / €420.00
  Acris Antibodies GmbH

MRP8/14 (S100A8/A9) antibody

MRP8/14 (S100A8/A9) Mouse IgM MRP8 7C12/4 Purified Hu E, F, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MRP8 / MRP14 antibody

SM2011F Human Macrophages antibody -FITC 
Staining of Human peripheral blood granulocytes. Mouse IgG1 MAC387 FITC Bov, Can, Eq, Fe, GP, Hu, Mky, Por, Rb, Rt F
0.1 mg / €410.00
  Acris Antibodies GmbH

MRP8 / MRP14 antibody

SM2011F Human Macrophages antibody -FITC 
Staining of Human peripheral blood granulocytes. Mouse IgG1 MAC387 FITC Bov, Can, Eq, Fe, GP, Hu, Mky, Por, Rb, Rt F
25 µg / €190.00
  Acris Antibodies GmbH

MRP8 / MRP14 antibody

Figure 1. SM2011P/PT Macrophages antibody staining of allergic marmoset brain using enhanced DAB. Mag. X400. Mouse IgG1 MAC387 Aff - Purified Bov, Can, Eq, Fe, GP, Hu, Mky, Por, Rb, Rt C, F, P
0.2 mg / €470.00
  Acris Antibodies GmbH

MRP8 / MRP14 antibody

Figure 1. SM2011P/PT Macrophages antibody staining of allergic marmoset brain using enhanced DAB. Mag. X400. Mouse IgG1 MAC387 Aff - Purified Bov, Can, Eq, Fe, GP, Hu, Mky, Por, Rb, Rt C, F, P
0.1 mg / €300.00
  Acris Antibodies GmbH

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