MRPL28 antibody

Principal name

MRPL28 antibody

Alternative names for MRPL28 antibody

L28mt, MAAT1, MRP-L28, Melanoma antigen p15, 39S ribosomal protein L28, mitochondrial, Melanoma-associated antigen recognized by T lymphocytes

SwissProt ID

P36527 (Yeast), Q13084 (Human), Q2HJJ1 (Bovin), Q9D1B9 (Mouse)

Gene ID

10573 (MRPL28)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of MRPL28 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rR composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rR. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was origilly isolated by its ability to recognize tyrosise in an HLA-A24-restricted fashion.

General readings

Koc EC, et al. (2001) J Biol Chem 276 (47): 43958–69.Kawakami, Y., et al. (2000) J.Immunother. 23: 17-27.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MRPL28 antibody

MRPL28 Antibody (N-term) (Cat. #TA324663) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MRPL28 antibody detected the MRPL28 protein (arrow). Rabbit IgG Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MRPL28 antibody

Immunofluorescence analysis of HeLa cell using MRPL28 antibody. Blue: DAPI for nuclear staining. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRPL28 antibody

WB Suggested Anti-MRPL28 Antibody Titration: 0.2-1 ug/ml; Positive Control: PANC1 cell lysateMRPL28 is supported by BioGPS gene expression data to be expressed in PANC1 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MRPL28 antibody

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: 293T cells, human testis tissue, A375 cells, human normal kidney tissue, Primary antibody: (MRPL28 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MRPL28 antibody

Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (MRPL28 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MRPL28 antibody

Western Blot analysis of MRPL28 expression in transfected 293T cell line (H00010573-T01) by MRPL28 MaxPab polyclonal antibody.

Lane 1: MRPL28 transfected lysate(14.90 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MRPL28 antibody

MRPL28 MaxPab polyclonal antibody. Western Blot analysis of MRPL28 expression in human placenta. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MRPL28 antibody

MRPL28 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MRPL28 antibody

Human PANC1; WB Suggested Anti-MRPL28 Antibody Titration: 0.2-1 ug/ml. Positive Control: PANC1 cell lysate. MRPL28 is supported by BioGPS gene expression data to be expressed in PANC1.; MRPL28 antibody - middle region (ARP52182_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MRPL28 antibody

Western blot analysis of MRPL28 expression in JAR (A); MCF7 (B); A549 (C); Raw264.7 (D); H9C2 (E) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MRPL28 antibody

Western blot analysis of MRPL28 expression in JAR (A); MCF7 (B); A549 (C); Raw264.7 (D); H9C2 (E) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MRPL28 antibody

Western blot analysis of MRPL28 expression in JAR (A); MCF7 (B); A549 (C); Raw264.7 (D); H9C2 (E) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

MRPL28 - human gene knockout kit via CRISPR

MRPL28 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MRPL28 - human gene knockout kit via CRISPR

MRPL28 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mrpl28 - mouse gene knockout kit via CRISPR

Mrpl28 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mrpl28 - mouse gene knockout kit via CRISPR

Mrpl28 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MRPL28

MRPL28 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MRPL28 (56-256, His-tag)

MRPL28 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

MRPL28 (56-256, His-tag)

MRPL28 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

MRPL28

MRPL28 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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